Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NIY84_RS04040 Genome accession   NZ_CP099914
Coordinates   840117..841097 (+) Length   326 a.a.
NCBI ID   WP_256878848.1    Uniprot ID   -
Organism   Escherichia albertii strain 104_2_TS_A     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 835117..846097
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIY84_RS04010 (NIY84_04010) - 835722..836729 (+) 1008 WP_131109251.1 DUF1202 family protein -
  NIY84_RS04015 (NIY84_04015) hemW 836794..837930 (-) 1137 WP_059273233.1 radical SAM family heme chaperone HemW -
  NIY84_RS04020 (NIY84_04020) - 837923..838516 (-) 594 WP_001174776.1 XTP/dITP diphosphatase -
  NIY84_RS04025 (NIY84_04025) yggU 838524..838814 (-) 291 WP_001277225.1 DUF167 family protein YggU -
  NIY84_RS04030 (NIY84_04030) yggT 838811..839377 (-) 567 WP_001094839.1 osmotic shock tolerance protein YggT -
  NIY84_RS04035 (NIY84_04035) yggS 839395..840099 (-) 705 WP_000997809.1 pyridoxal phosphate homeostasis protein -
  NIY84_RS04040 (NIY84_04040) pilT 840117..841097 (+) 981 WP_256878848.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NIY84_RS04045 (NIY84_04045) ruvX 841281..841697 (-) 417 WP_000017099.1 Holliday junction resolvase RuvX -
  NIY84_RS04050 (NIY84_04050) - 841697..842260 (-) 564 WP_002461296.1 YqgE/AlgH family protein -
  NIY84_RS04055 (NIY84_04055) gshB 842369..843319 (-) 951 WP_000593300.1 glutathione synthase -
  NIY84_RS04060 (NIY84_04060) rsmE 843332..844063 (-) 732 WP_002461297.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NIY84_RS04065 (NIY84_04065) endA 844143..844850 (-) 708 WP_002461298.1 deoxyribonuclease I -
  NIY84_RS04070 (NIY84_04070) - 844945..845442 (-) 498 WP_054410340.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35958.12 Da        Isoelectric Point: 5.9935

>NTDB_id=702445 NIY84_RS04040 WP_256878848.1 840117..841097(+) (pilT) [Escherichia albertii strain 104_2_TS_A]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEVLLREWLDDAQRALLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKNHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=702445 NIY84_RS04040 WP_256878848.1 840117..841097(+) (pilT) [Escherichia albertii strain 104_2_TS_A]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGCCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACGTTGAAGTGCTGCTAAGGGAATGGCTGGATG
ACGCTCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGATTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGCCCGCAACTCGAACAACTTGG
TGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTAATTTTGCTTGGCGAGCTGCGGGACAGCGAGACTATTCGCCTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCGGCTCAGGCGGTCGAGCGGCTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCGGGTAGTTTACGGGCAGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAAACCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

41.009

97.239

0.399
  pilU Pseudomonas stutzeri DSM 10701

38.253

100

0.39