Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NH234_RS25470 Genome accession   NZ_CP099760
Coordinates   5563246..5563683 (-) Length   145 a.a.
NCBI ID   WP_367254661.1    Uniprot ID   -
Organism   Pseudomonas sp. stari2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5558246..5568683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH234_RS25455 (NH234_25430) - 5559177..5562128 (+) 2952 WP_367254659.1 DUF748 domain-containing protein -
  NH234_RS25460 (NH234_25435) - 5562128..5562424 (+) 297 WP_085732827.1 DUF2845 domain-containing protein -
  NH234_RS25465 (NH234_25440) - 5562593..5562949 (-) 357 WP_085732828.1 BON domain-containing protein -
  NH234_RS25470 (NH234_25445) pilA 5563246..5563683 (-) 438 WP_367254661.1 pilin Machinery gene
  NH234_RS25475 (NH234_25450) pilB 5563910..5565610 (+) 1701 WP_085732830.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NH234_RS25480 (NH234_25455) pilC 5565613..5566830 (+) 1218 WP_367254663.1 type II secretion system F family protein Machinery gene
  NH234_RS25485 (NH234_25460) pilD 5566832..5567704 (+) 873 WP_085732832.1 A24 family peptidase Machinery gene
  NH234_RS25490 (NH234_25465) coaE 5567701..5568324 (+) 624 WP_085732833.1 dephospho-CoA kinase -
  NH234_RS25495 (NH234_25470) yacG 5568321..5568521 (+) 201 WP_085712167.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14640.70 Da        Isoelectric Point: 8.4936

>NTDB_id=701569 NH234_RS25470 WP_367254661.1 5563246..5563683(-) (pilA) [Pseudomonas sp. stari2]
MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISAMKVPFEDTINQGTAPDINSVNGGTTTTSNCTV
QVTGTAATGAGSISCTLLNAPGPVLGKTVTLTRSGAATGNTSGVWTCATTVNPDYSPKGCTASGT

Nucleotide


Download         Length: 438 bp        

>NTDB_id=701569 NH234_RS25470 WP_367254661.1 5563246..5563683(-) (pilA) [Pseudomonas sp. stari2]
ATGAAAAAACAACAAGGTTTCACTCTGATCGAGCTGCTGATTGTCGTGGCGATCATCGGCATCCTGGCCACCATCGCCTT
GCCGCAGTATTCCAAGTATCAGGCACGGTCGAAGGTGACGGCGGGGCTGGCGGAAATCAGTGCGATGAAGGTGCCGTTCG
AGGACACCATCAATCAAGGTACCGCTCCGGATATAAACTCTGTGAACGGCGGTACAACCACCACCTCCAACTGTACGGTG
CAGGTTACAGGTACAGCGGCGACGGGGGCGGGCAGCATCTCCTGCACGCTGCTCAACGCTCCGGGGCCGGTACTGGGCAA
AACTGTCACGCTCACTCGTTCGGGCGCTGCTACCGGTAATACCTCTGGTGTGTGGACCTGTGCCACTACTGTTAACCCGG
ATTATTCGCCAAAAGGCTGTACCGCCAGCGGTACGTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.276

100

0.483
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.853

98.621

0.462
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.29

100

0.441
  pilA Vibrio cholerae strain A1552

41.29

100

0.441
  pilA Vibrio cholerae C6706

41.29

100

0.441
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.262

97.241

0.421
  comP Acinetobacter baylyi ADP1

39.216

100

0.414
  pilA Pseudomonas aeruginosa PAK

37.736

100

0.414
  pilA Ralstonia pseudosolanacearum GMI1000

35.976

100

0.407
  pilA Vibrio parahaemolyticus RIMD 2210633

39.716

97.241

0.386
  pilA2 Legionella pneumophila strain ERS1305867

37.857

96.552

0.366
  pilA2 Legionella pneumophila str. Paris

37.857

96.552

0.366