Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NH234_RS25480 Genome accession   NZ_CP099760
Coordinates   5565613..5566830 (+) Length   405 a.a.
NCBI ID   WP_367254663.1    Uniprot ID   -
Organism   Pseudomonas sp. stari2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5560613..5571830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH234_RS25460 (NH234_25435) - 5562128..5562424 (+) 297 WP_085732827.1 DUF2845 domain-containing protein -
  NH234_RS25465 (NH234_25440) - 5562593..5562949 (-) 357 WP_085732828.1 BON domain-containing protein -
  NH234_RS25470 (NH234_25445) pilA 5563246..5563683 (-) 438 WP_367254661.1 pilin Machinery gene
  NH234_RS25475 (NH234_25450) pilB 5563910..5565610 (+) 1701 WP_085732830.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NH234_RS25480 (NH234_25455) pilC 5565613..5566830 (+) 1218 WP_367254663.1 type II secretion system F family protein Machinery gene
  NH234_RS25485 (NH234_25460) pilD 5566832..5567704 (+) 873 WP_085732832.1 A24 family peptidase Machinery gene
  NH234_RS25490 (NH234_25465) coaE 5567701..5568324 (+) 624 WP_085732833.1 dephospho-CoA kinase -
  NH234_RS25495 (NH234_25470) yacG 5568321..5568521 (+) 201 WP_085712167.1 DNA gyrase inhibitor YacG -
  NH234_RS25500 (NH234_25475) - 5568528..5568743 (-) 216 WP_367254665.1 hypothetical protein -
  NH234_RS25505 (NH234_25480) - 5568809..5569498 (-) 690 WP_065261574.1 energy-coupling factor ABC transporter permease -
  NH234_RS25510 (NH234_25485) - 5569632..5570258 (+) 627 WP_007959580.1 DUF1780 domain-containing protein -
  NH234_RS25515 (NH234_25490) - 5570255..5570782 (+) 528 WP_367254666.1 MOSC domain-containing protein -
  NH234_RS25520 (NH234_25495) - 5570834..5571007 (+) 174 WP_065261571.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44078.87 Da        Isoelectric Point: 10.1093

>NTDB_id=701571 NH234_RS25480 WP_367254663.1 5565613..5566830(+) (pilC) [Pseudomonas sp. stari2]
MAVKAAKISVYAWEGTDKKGSRISGELSGQNPALIKAQLRKQGINPGKVRKKSASLLNFGKRIKPLDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ESLKAKIKKAMTYPTAVILVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVIGLSEFMQQWWWAILGVLIVAFFGTR
HALKKSQALRDRRDAWLLKLPLVGTMMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVATGM
QLNFSMRTSGAFPNMAVQMTAIGEESGALDDMLDKVAGFYEEEVDNMVDNLTSLMEPFVMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=701571 NH234_RS25480 WP_367254663.1 5565613..5566830(+) (pilC) [Pseudomonas sp. stari2]
ATGGCCGTCAAGGCAGCGAAAATCAGCGTCTACGCCTGGGAAGGAACCGACAAGAAAGGCAGCCGGATCAGCGGCGAACT
GAGCGGGCAAAACCCCGCGCTGATCAAGGCCCAGTTGCGCAAGCAGGGCATCAACCCCGGCAAGGTACGCAAGAAATCCG
CGTCGCTGCTGAACTTCGGCAAACGCATCAAGCCGCTGGACATCGCCCTCTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCATTGCTGCAGTCGTTCGACATCATCGGCGAAGGCTTTGAAAATCCGGCGATGCGCAAACTGGTGGACGA
GGTGAAACAGGAGGTCGCCGCCGGTAACAGCTTCGCCGCGGCCCTGCGCAAGAAGCCGCAATACTTCGACGAGTTGTACT
GCAACCTGGTCGATGCCGGCGAGCAATCCGGTGCTCTCGACACCCTGCTCGAGCGCGTGGCGACCTACAAGGAGAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACCTATCCCACCGCCGTGATTCTGGTGGCGGCGGTCGTCACCGGCAT
TCTGCTGGTAAAAGTGGTGCCGCAGTTCCAGTCGGTATTTTCCGGCTTCGGTGCCGAACTGCCGGCGTTTACCTTGATGG
TCATCGGCCTGTCGGAGTTCATGCAGCAATGGTGGTGGGCGATTCTGGGTGTGCTGATCGTGGCGTTTTTCGGCACCCGC
CATGCCTTGAAGAAATCCCAGGCCCTGCGTGACCGACGTGATGCCTGGCTGCTGAAACTGCCACTGGTGGGCACCATGAT
GTACAAGTCCGCCGTCGCCCGTTTTGCCCGGACCCTGTCGACCACGTTTGCCGCCGGCGTGCCGCTGGTCGAAGCGCTGG
ACTCGGTCGCCGGAGCCACCGGCAACGTGGTGTTCAAGCGTGCGGTGCTGCGCATTCGCCAGGACGTGGCCACCGGCATG
CAGCTGAATTTCTCGATGCGCACCAGTGGGGCTTTTCCGAACATGGCCGTGCAGATGACCGCCATCGGCGAAGAGTCCGG
CGCCCTGGACGATATGCTCGACAAGGTAGCCGGGTTCTACGAGGAAGAAGTGGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCTTTCGTCATGGTGGTACTCGGGGTGATCGTCGGCGGGCTGGTCGTGGCCATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.58

100

0.736
  pilC Acinetobacter baumannii D1279779

59.069

100

0.595
  pilC Acinetobacter baylyi ADP1

58.824

100

0.593
  pilC Legionella pneumophila strain ERS1305867

53.5

98.765

0.528
  pilG Neisseria gonorrhoeae MS11

42.68

99.506

0.425
  pilC Vibrio cholerae strain A1552

43.434

97.778

0.425
  pilG Neisseria meningitidis 44/76-A

42.184

99.506

0.42
  pilC Vibrio campbellii strain DS40M4

41.032

100

0.412
  pilC Thermus thermophilus HB27

38.213

99.506

0.38