Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NH234_RS25475 Genome accession   NZ_CP099760
Coordinates   5563910..5565610 (+) Length   566 a.a.
NCBI ID   WP_085732830.1    Uniprot ID   -
Organism   Pseudomonas sp. stari2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5558910..5570610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH234_RS25455 (NH234_25430) - 5559177..5562128 (+) 2952 WP_367254659.1 DUF748 domain-containing protein -
  NH234_RS25460 (NH234_25435) - 5562128..5562424 (+) 297 WP_085732827.1 DUF2845 domain-containing protein -
  NH234_RS25465 (NH234_25440) - 5562593..5562949 (-) 357 WP_085732828.1 BON domain-containing protein -
  NH234_RS25470 (NH234_25445) pilA 5563246..5563683 (-) 438 WP_367254661.1 pilin Machinery gene
  NH234_RS25475 (NH234_25450) pilB 5563910..5565610 (+) 1701 WP_085732830.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NH234_RS25480 (NH234_25455) pilC 5565613..5566830 (+) 1218 WP_367254663.1 type II secretion system F family protein Machinery gene
  NH234_RS25485 (NH234_25460) pilD 5566832..5567704 (+) 873 WP_085732832.1 A24 family peptidase Machinery gene
  NH234_RS25490 (NH234_25465) coaE 5567701..5568324 (+) 624 WP_085732833.1 dephospho-CoA kinase -
  NH234_RS25495 (NH234_25470) yacG 5568321..5568521 (+) 201 WP_085712167.1 DNA gyrase inhibitor YacG -
  NH234_RS25500 (NH234_25475) - 5568528..5568743 (-) 216 WP_367254665.1 hypothetical protein -
  NH234_RS25505 (NH234_25480) - 5568809..5569498 (-) 690 WP_065261574.1 energy-coupling factor ABC transporter permease -
  NH234_RS25510 (NH234_25485) - 5569632..5570258 (+) 627 WP_007959580.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62553.54 Da        Isoelectric Point: 5.5555

>NTDB_id=701570 NH234_RS25475 WP_085732830.1 5563910..5565610(+) (pilB) [Pseudomonas sp. stari2]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLTTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDG
LDVESVDDSKQDAIGVLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCGHCKKAIEIPRETLLKEGFPE
ERIGSFTIYEPVGCDQCNGGYKGRTGIYEVVRNTPELQRLIMAEGNSLEIDSQMRLDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=701570 NH234_RS25475 WP_085732830.1 5563910..5565610(+) (pilB) [Pseudomonas sp. stari2]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTACAGGCCGAACTGCTCACGGAAAAAAGCGCCCAACAAGC
CTGGCAGCAAGCCCAGCGCAATCGCCTGTCGCTGGTCAGCTATCTGGTACAGAACAAACTGGTGAAGAGTTCGCAGGTTG
CCGAAATCGCCTCGGAGCACTTCGGCATGGCGTTCCTCGACCTCAATTGCCTGGACAAGGAAACCCAGCCCAAGGGCCTG
GTCAGCGAAAAACTGGTCCGCCAGCATCATGCCCTGCCCCTGTGGCGGCGCGGCAACAAACTGTTCGTGGGGATTTCCGA
CCCGAGCAATCATCAGGCAATCAACGACATTCAGTTCAGCACCGGGCTGACCACCGAAGCCATTCTGGTGGAGGACGATA
AGCTCACCGATGCCATCGAGAAATTCTTCGACACCCACGCCTCCGGCCTGGAAGACATGGCCGATGTCGACCTCGACGGA
CTGGACGTCGAATCCGTCGACGACAGCAAGCAGGACGCCATCGGCGTGCTGGATGCCGACGACGCGCCGGTGGTGCGCTT
CGTCCACAAGATGCTGCTGGACGCAATCAAGAGCGGCTCTTCCGACCTGCACTTCGAGCCTTACGAGAAAAACTACCGGG
TGCGGGTACGTACCGACGGCATCCTGCGTGAGGTGGCCAAACCGCCGATTCAACTGGCCGGACGCATCGCCGCACGCCTG
AAGGTCATGGCCAGCCTCGACATTTCGGAACGGCGCAAGCCTCAGGACGGACGCCTGAAGATGCGCCTGTCGAAAACCAA
GTCGATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTAATTCGGATCCTCGACCCTTCCAGCG
CGCAGATCGGCATCGACGCCCTCGGCTACGAGCCGGAGCAGAAAGAGCTGTACCTGGCCGCCCTGAAGCAGCCGCAAGGC
ATGATTCTGGTCACCGGCCCCACCGGTTCCGGCAAGACCGTGTCGCTGTACACCGGGCTGAACATCCTCAATACCGTCGA
CATCAATATCTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGTATCAACCAGGTCAACGTCAATCCGAAGCAGG
GGCTGGATTTCGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACGGCGGAAATCGCCATCAAGGCGGCGCAGACCGGTCACCTTGTACTGTCCACGCTGCATACCAACAGCGCAGCGGA
AACGCTGACCCGCTTGCACAACATGGGCATTCCCGGCTTCAACATTGCCACGTCGGTCAGCCTGATCATCGCCCAGCGTC
TGGCGCGCAAACTCTGCGGCCACTGCAAGAAAGCCATCGAGATTCCCCGCGAAACCCTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCAGTTTCACGATCTATGAGCCCGTCGGTTGCGATCAATGCAACGGCGGCTACAAGGGCCGCACCGGTAT
CTATGAAGTGGTCAGGAACACTCCCGAACTGCAGCGGCTGATCATGGCCGAGGGCAACTCACTGGAAATCGACAGCCAGA
TGCGCCTGGACGGCTTCAACGATCTGCGCACTTCGGGCTTGATCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.699

99.823

0.576
  pilB Acinetobacter baumannii D1279779

57.27

99.647

0.571
  pilB Legionella pneumophila strain ERS1305867

56.338

100

0.565
  pilB Vibrio cholerae strain A1552

51.327

99.823

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

50.356

99.293

0.5
  pilF Neisseria gonorrhoeae MS11

49.556

99.47

0.493
  pilB Vibrio campbellii strain DS40M4

48.947

100

0.493
  pilF Thermus thermophilus HB27

36.918

98.587

0.364