Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   NF677_RS22440 Genome accession   NZ_CP099599
Coordinates   5029266..5029688 (-) Length   140 a.a.
NCBI ID   WP_041477820.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6ESPEB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5024266..5034688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF677_RS22425 (NF677_22425) - 5025105..5028056 (+) 2952 WP_252884845.1 DUF748 domain-containing protein -
  NF677_RS22430 (NF677_22430) - 5028053..5028352 (+) 300 WP_150742242.1 DUF2845 domain-containing protein -
  NF677_RS22435 (NF677_22435) - 5028617..5028970 (-) 354 WP_252884846.1 BON domain-containing protein -
  NF677_RS22440 (NF677_22440) pilA/pilAI 5029266..5029688 (-) 423 WP_041477820.1 pilin Machinery gene
  NF677_RS22445 (NF677_22445) pilB 5029915..5031615 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NF677_RS22450 (NF677_22450) pilC 5031618..5032835 (+) 1218 WP_252884847.1 type II secretion system F family protein Machinery gene
  NF677_RS22455 (NF677_22455) pilD 5032837..5033709 (+) 873 WP_252884848.1 A24 family peptidase Machinery gene
  NF677_RS22460 (NF677_22460) coaE 5033706..5034329 (+) 624 WP_252884849.1 dephospho-CoA kinase -
  NF677_RS22465 (NF677_22465) yacG 5034326..5034526 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14144.32 Da        Isoelectric Point: 8.4918

>NTDB_id=700932 NF677_RS22440 WP_041477820.1 5029266..5029688(-) (pilA/pilAI) [Pseudomonas siliginis strain OTU6ESPEB1]
MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISAMKVPFEDLINQGTNPSLAAVKGDNVTTTSNCA
LTASGTAADGAGTIVCTLANAPAPVLGKTITLTRTAAGGWSCGTTVAPDYSPKGCNATGT

Nucleotide


Download         Length: 423 bp        

>NTDB_id=700932 NF677_RS22440 WP_041477820.1 5029266..5029688(-) (pilA/pilAI) [Pseudomonas siliginis strain OTU6ESPEB1]
ATGAAAAAACAACAAGGCTTTACATTGATCGAGCTGCTGATCGTTGTGGCGATCATCGGCATTCTGGCGACTATCGCCCT
GCCGCAGTATTCGAAGTATCAGGCGCGGTCGAAGGTGACGGCGGGGCTGGCGGAGATCAGCGCGATGAAGGTGCCGTTTG
AAGACCTGATCAATCAGGGGACAAATCCAAGCTTGGCGGCGGTGAAAGGCGATAACGTCACCACTACCTCCAATTGCGCT
CTGACTGCCTCAGGTACCGCTGCAGACGGAGCTGGAACGATTGTATGTACGTTGGCCAACGCGCCAGCGCCTGTATTGGG
CAAAACCATCACGCTGACACGTACAGCTGCGGGTGGTTGGAGCTGTGGCACTACGGTAGCTCCTGACTATTCACCGAAAG
GCTGTAACGCTACTGGTACTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50.365

97.857

0.493
  pilA Acinetobacter baumannii strain A118

46.763

99.286

0.464
  pilA Vibrio cholerae strain A1552

41.29

100

0.457
  pilA Vibrio cholerae C6706

41.29

100

0.457
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.29

100

0.457
  comP Acinetobacter baylyi ADP1

41.447

100

0.45
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.443
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

96.429

0.429
  pilA2 Legionella pneumophila str. Paris

39.041

100

0.407
  pilA Vibrio parahaemolyticus RIMD 2210633

42.222

96.429

0.407
  pilA2 Legionella pneumophila strain ERS1305867

36.986

100

0.386
  pilA/pilA1 Eikenella corrodens VA1

39.259

96.429

0.379