Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NF677_RS22450 Genome accession   NZ_CP099599
Coordinates   5031618..5032835 (+) Length   405 a.a.
NCBI ID   WP_252884847.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6ESPEB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5026618..5037835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF677_RS22430 (NF677_22430) - 5028053..5028352 (+) 300 WP_150742242.1 DUF2845 domain-containing protein -
  NF677_RS22435 (NF677_22435) - 5028617..5028970 (-) 354 WP_252884846.1 BON domain-containing protein -
  NF677_RS22440 (NF677_22440) pilA/pilAI 5029266..5029688 (-) 423 WP_041477820.1 pilin Machinery gene
  NF677_RS22445 (NF677_22445) pilB 5029915..5031615 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NF677_RS22450 (NF677_22450) pilC 5031618..5032835 (+) 1218 WP_252884847.1 type II secretion system F family protein Machinery gene
  NF677_RS22455 (NF677_22455) pilD 5032837..5033709 (+) 873 WP_252884848.1 A24 family peptidase Machinery gene
  NF677_RS22460 (NF677_22460) coaE 5033706..5034329 (+) 624 WP_252884849.1 dephospho-CoA kinase -
  NF677_RS22465 (NF677_22465) yacG 5034326..5034526 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  NF677_RS22470 (NF677_22470) - 5034534..5034749 (-) 216 WP_016773148.1 hypothetical protein -
  NF677_RS22475 (NF677_22475) - 5034815..5035504 (-) 690 WP_041477814.1 energy-coupling factor ABC transporter permease -
  NF677_RS22480 (NF677_22480) - 5035695..5036321 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  NF677_RS22485 (NF677_22485) - 5036318..5036845 (+) 528 WP_252882699.1 MOSC domain-containing protein -
  NF677_RS22490 (NF677_22490) - 5036897..5037070 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44071.77 Da        Isoelectric Point: 10.1214

>NTDB_id=700934 NF677_RS22450 WP_252884847.1 5031618..5032835(+) (pilC) [Pseudomonas siliginis strain OTU6ESPEB1]
MAVKAAKVSVYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLTFGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAVFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=700934 NF677_RS22450 WP_252884847.1 5031618..5032835(+) (pilC) [Pseudomonas siliginis strain OTU6ESPEB1]
ATGGCGGTCAAGGCAGCGAAAGTCAGCGTTTACGCCTGGGAAGGCACCGACCGCAAAGGCAGTAAAGTCAGCGGCGAGTT
GAGCGGACAGAACCCCGCACTGATCAAGGCGCAGCTGCGCAAACAGGGGATCAACCCGGGCAAGGTGCGCAAGAAATCCG
CGTCCCTGCTGACCTTCGGCAAACGCATCAAACCGCAGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGCTGTTGCAGTCCTTCGACATCATCGGCGAAGGCTTCGAAAACCCGGCCATGCGCAAACTGGTCGACGA
GGTCAAACAGGAGGTCGCGGCCGGTAACAGCTTCGCTACGGCCCTGCGCAAGAAACCGCAATACTTCGACGAGTTGTACT
GCAATCTGGTCGACGCCGGCGAACAGTCTGGCGCCCTCGACACCTTGCTTGAACGTGTGGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCCACCGCCGTCGTGCTGGTGGCGGCAGTGGTAACAGGCAT
TCTGCTGGTCAAAGTGGTGCCGCAGTTCCAGTCGGTATTCTCCGGGTTCGGCGCCGAACTGCCGGGCTTCACCCTGATGA
TCATCAGCCTGTCGGAATTCATGCAGCAGTGGTGGTGGGCCATTCTTTTTGCGCTGGTGGCGGTGTTTTTCGGCACTCGC
CACGCGCTGAAGACATCGCAGGCCCTGCGTGACCGCCGCGATGCCTGGCTGTTGAAGCTGCCACTGGTGGGCACCTTGAT
GTACAAGTCCGCCGTCGCCCGCTTTGCCCGCACCCTGTCGACCACATTCGCAGCCGGTGTGCCGCTGGTGGAAGCACTGG
ATTCGGTCGCCGGTGCCACCGGCAATGTGGTGTTCAAGCGCGCGGTGTTGCGGGTGCGGCAGGACGTCTCGACCGGCATG
CAACTGAATTTTTCGATGCGCAGCACCGGTGTGTTTCCGAACATGGCGGTGCAGATGACCGCCATCGGCGAGGAGTCCGG
GGCACTGGATGACATGCTCGACAAGGTCGCCGGTTTTTATGAAGCGGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGTGGACTGGTGGTGGCCATGTACCTGCCGATCTTCCAG
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.827

100

0.738
  pilC Acinetobacter baumannii D1279779

58.824

100

0.593
  pilC Acinetobacter baylyi ADP1

58.578

100

0.59
  pilC Legionella pneumophila strain ERS1305867

53

98.765

0.523
  pilC Vibrio cholerae strain A1552

44.444

97.778

0.435
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

41.872

100

0.42
  pilC Thermus thermophilus HB27

39.599

98.519

0.39