Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NF677_RS22445 Genome accession   NZ_CP099599
Coordinates   5029915..5031615 (+) Length   566 a.a.
NCBI ID   WP_056790439.1    Uniprot ID   A0AAQ3G636
Organism   Pseudomonas siliginis strain OTU6ESPEB1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5024915..5036615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF677_RS22425 (NF677_22425) - 5025105..5028056 (+) 2952 WP_252884845.1 DUF748 domain-containing protein -
  NF677_RS22430 (NF677_22430) - 5028053..5028352 (+) 300 WP_150742242.1 DUF2845 domain-containing protein -
  NF677_RS22435 (NF677_22435) - 5028617..5028970 (-) 354 WP_252884846.1 BON domain-containing protein -
  NF677_RS22440 (NF677_22440) pilA/pilAI 5029266..5029688 (-) 423 WP_041477820.1 pilin Machinery gene
  NF677_RS22445 (NF677_22445) pilB 5029915..5031615 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NF677_RS22450 (NF677_22450) pilC 5031618..5032835 (+) 1218 WP_252884847.1 type II secretion system F family protein Machinery gene
  NF677_RS22455 (NF677_22455) pilD 5032837..5033709 (+) 873 WP_252884848.1 A24 family peptidase Machinery gene
  NF677_RS22460 (NF677_22460) coaE 5033706..5034329 (+) 624 WP_252884849.1 dephospho-CoA kinase -
  NF677_RS22465 (NF677_22465) yacG 5034326..5034526 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  NF677_RS22470 (NF677_22470) - 5034534..5034749 (-) 216 WP_016773148.1 hypothetical protein -
  NF677_RS22475 (NF677_22475) - 5034815..5035504 (-) 690 WP_041477814.1 energy-coupling factor ABC transporter permease -
  NF677_RS22480 (NF677_22480) - 5035695..5036321 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62620.67 Da        Isoelectric Point: 6.1319

>NTDB_id=700933 NF677_RS22445 WP_056790439.1 5029915..5031615(+) (pilB) [Pseudomonas siliginis strain OTU6ESPEB1]
MNDIALSGLAKQLVQAELLTETSARQSWAQAQRNRVSLVNYLVHNKLVNSRQIAEIASEHFGMALLDLHCLDKETQPKGL
VSEKLVRQHCALPLWRRGNKLFVGLSDPSNQQAISDIQFSTGLSTEAILVEDDKLSDAIDKFFDSHASGLEEMADVDLDG
LDVESIDDNKQDVIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRMRTDGMLREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEAEQKALYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCAHCKRPLEIPHETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFADLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=700933 NF677_RS22445 WP_056790439.1 5029915..5031615(+) (pilB) [Pseudomonas siliginis strain OTU6ESPEB1]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAATTGCTCACGGAAACAAGCGCCCGGCAATC
CTGGGCGCAGGCCCAGCGCAATCGCGTGTCGCTGGTCAATTACCTGGTGCACAACAAACTGGTCAACAGCCGGCAAATTG
CCGAGATCGCCTCGGAACATTTCGGCATGGCCCTGCTCGATCTGCATTGCCTCGACAAAGAGACTCAGCCCAAGGGTCTG
GTCAGTGAGAAACTGGTTCGCCAGCACTGCGCCCTGCCCCTCTGGCGGCGCGGCAACAAACTGTTCGTGGGCCTTTCCGA
CCCAAGCAATCAGCAGGCGATCAGCGACATCCAGTTCAGCACCGGCCTGAGCACCGAAGCGATTCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGACAAGTTCTTCGACAGCCATGCCTCGGGCCTGGAGGAAATGGCCGATGTCGATCTCGACGGC
CTCGACGTCGAGTCCATTGATGACAACAAACAGGACGTGATCGGCGGCCTCGATGCCGACGACGCCCCGGTCGTACGTTT
TGTGCACAAGATGCTGCTCGATGCGATCAAAAGCGGCTCTTCCGACCTGCATTTCGAACCCTACGAAAAAAACTATCGGG
TGCGCATGCGCACCGACGGCATGCTGCGCGAAGTGGCCAAGCCGCCGATCCAGTTGGCCGGGCGCATTGCCGCGCGCTTG
AAGGTAATGGCCAGTCTGGATATTTCCGAACGGCGCAAACCTCAGGACGGCCGCATCAAAATGCGCCTGTCGAAAAGCAA
ATCCATCGATTTCCGCGTCAACACCCTGCCGACTCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGTG
CGCAAATGGGCATCGATGCGCTGGGCTACGAGGCCGAACAAAAAGCCTTGTACCTGGCCGCGCTGAAACAGCCGCAAGGG
ATGATTCTGGTCACCGGCCCCACCGGCTCCGGTAAAACCGTGTCGCTGTACACCGGGCTGAACATCCTCAACACCGTCGA
CATCAATATTTCCACCGCCGAAGATCCGGTGGAAATCAACATGGAAGGCATCAATCAGGTCAACGTCAATCCCCGCCAAG
GGCTGGACTTTGCCCAGGCGCTGCGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCAATCAAGGCTGCGCAGACCGGCCACCTGGTGCTGTCGACCCTGCACACCAATAGCGCAGCGGA
AACCCTGACACGCCTGCACAACATGGGCATTCCCGGTTTCAACATCGCCACCTCGGTCAGCCTGATCATCGCCCAGCGTC
TGGCGCGCAAGTTGTGCGCACACTGCAAGCGGCCGCTGGAGATCCCGCACGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAATGGCGGTTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTAAAGAACACACCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCGCTGGAAATCGATATTCAGA
TGCGCCGCGACGGCTTCGCCGATCTGCGCACTTCCGGGCTCCACAAGGCCATGCAAGGCATCACCAGCCTTGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.699

99.823

0.576
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

56.537

100

0.565
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.446

99.117

0.5
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.209

100

0.495
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.837

94.17

0.366