Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NG743_RS26260 Genome accession   NZ_CP099464
Coordinates   5758268..5759368 (+) Length   366 a.a.
NCBI ID   WP_053539894.1    Uniprot ID   -
Organism   Dolichospermum heterosporum TAC447 strain NIES-1697     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5753268..5764368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG743_RS26240 (NG743_26110) - 5753727..5754326 (-) 600 WP_027401139.1 hypothetical protein -
  NG743_RS26245 (NG743_26115) - 5754458..5754613 (-) 156 WP_160170591.1 hypothetical protein -
  NG743_RS26250 (NG743_26120) grpE 5754886..5755560 (-) 675 WP_027401138.1 nucleotide exchange factor GrpE -
  NG743_RS26255 (NG743_26125) pilB/pilB1 5756203..5758203 (+) 2001 WP_027401137.1 GspE/PulE family protein Machinery gene
  NG743_RS26260 (NG743_26130) pilT 5758268..5759368 (+) 1101 WP_053539894.1 type IV pilus twitching motility protein PilT Machinery gene
  NG743_RS26265 (NG743_26135) - 5759445..5760668 (+) 1224 WP_027401135.1 type II secretion system F family protein -
  NG743_RS26270 (NG743_26140) - 5760758..5761318 (+) 561 WP_257121197.1 hypothetical protein -
  NG743_RS26275 (NG743_26145) - 5761417..5762955 (+) 1539 WP_051424227.1 thymidylate synthase -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40177.43 Da        Isoelectric Point: 7.1091

>NTDB_id=699833 NG743_RS26260 WP_053539894.1 5758268..5759368(+) (pilT) [Dolichospermum heterosporum TAC447 strain NIES-1697]
MEMMIEDLMEQLINMGGSDLHLSAGLPPYFRISGHLTPIGEHTLTADECQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAFAACLRALSSKIPNFEKLGLPDIVREMTEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILTV
EDPVEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETIALAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPSERQTQVRVQLSNSLVAVFSQTLVPKKNPKANEYGRVMAQEIMVITPAISNLIREGKTAQIYSAIQTGGKLGM
QTLEKVLADLYKAGTISFEAAISKTSKPDEVQRLIGATAPAGAKLR

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=699833 NG743_RS26260 WP_053539894.1 5758268..5759368(+) (pilT) [Dolichospermum heterosporum TAC447 strain NIES-1697]
ATGGAAATGATGATTGAAGACTTAATGGAGCAACTAATTAATATGGGTGGCTCAGACTTACACCTATCTGCTGGTTTGCC
TCCCTATTTTCGGATTAGTGGACATTTAACACCTATTGGTGAACATACCTTAACTGCTGATGAGTGCCAAAGGTTAATTT
TTAGTATGCTCAATAACACTCAGCGCAAAACCTTAGAGCAAACCTGGGAATTAGATTGTTCTTATGGTGTAAAAGGACTA
GCTCGGTTTCGGGTGAATGTCTACAAAGAGCGGGGCGCTTTTGCTGCTTGTTTACGAGCATTAAGTTCTAAAATTCCTAA
CTTTGAAAAATTAGGTTTGCCCGATATAGTTCGGGAAATGACGGAAAAACCTAGAGGATTAATTCTCGTTACAGGTCCAA
CTGGTTCTGGTAAAACTACTACCCTAGCGGCAATGATTGACCTAATTAATCGGACTAGGGCAGAACACATTTTAACAGTT
GAAGATCCTGTAGAATTTGTTTATGAACCTATCAAAAGTCTAGTTCACCAACGCCAATTAGGGGAAGATACGAAGAGTTT
TGCTAATGCTTTGAAAGCAGCTTTACGGGAAGATCCAGATATTATTCTCGTGGGAGAAATGCGGGATTTAGAAACAATTG
CTTTGGCAATTTCCGCCGCAGAAACAGGTCACTTGGTATTTGGTACATTACATACAAGCTCTGCTGCACAAACCGTTGAC
CGGATTATTGACGTTTTCCCCTCAGAAAGACAAACTCAGGTGCGGGTGCAGTTATCTAACTCCTTAGTTGCAGTTTTTAG
TCAAACTTTAGTACCTAAGAAAAATCCCAAAGCTAATGAATATGGTCGAGTTATGGCTCAAGAAATCATGGTGATTACTC
CCGCTATTTCTAACTTGATTCGGGAAGGAAAAACAGCACAGATTTATTCAGCTATTCAGACTGGTGGTAAATTGGGAATG
CAAACTCTAGAAAAGGTTTTGGCTGATTTATACAAAGCTGGAACTATTTCTTTTGAAGCGGCAATCTCTAAAACCTCTAA
ACCAGATGAAGTCCAGCGTCTAATTGGTGCGACAGCCCCAGCAGGAGCTAAATTGCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.467

94.809

0.516
  pilT Pseudomonas stutzeri DSM 10701

54.179

94.809

0.514
  pilT Legionella pneumophila strain ERS1305867

53.89

94.809

0.511
  pilT Acinetobacter baylyi ADP1

53.89

94.809

0.511
  pilT Legionella pneumophila strain Lp02

53.89

94.809

0.511
  pilT Acinetobacter nosocomialis M2

53.026

94.809

0.503
  pilT Acinetobacter baumannii D1279779

53.026

94.809

0.503
  pilT Acinetobacter baumannii strain A118

53.026

94.809

0.503
  pilT Neisseria gonorrhoeae MS11

51.622

92.623

0.478
  pilT Neisseria meningitidis 8013

51.327

92.623

0.475
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.292

93.443

0.47
  pilT Vibrio cholerae strain A1552

50.292

93.443

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.857

95.628

0.467
  pilU Acinetobacter baylyi ADP1

37.432

100

0.374