Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NG743_RS26255 Genome accession   NZ_CP099464
Coordinates   5756203..5758203 (+) Length   666 a.a.
NCBI ID   WP_027401137.1    Uniprot ID   -
Organism   Dolichospermum heterosporum TAC447 strain NIES-1697     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5751203..5763203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG743_RS26235 (NG743_26105) - 5752849..5753364 (-) 516 WP_257121196.1 TniQ family protein -
  NG743_RS26240 (NG743_26110) - 5753727..5754326 (-) 600 WP_027401139.1 hypothetical protein -
  NG743_RS26245 (NG743_26115) - 5754458..5754613 (-) 156 WP_160170591.1 hypothetical protein -
  NG743_RS26250 (NG743_26120) grpE 5754886..5755560 (-) 675 WP_027401138.1 nucleotide exchange factor GrpE -
  NG743_RS26255 (NG743_26125) pilB/pilB1 5756203..5758203 (+) 2001 WP_027401137.1 GspE/PulE family protein Machinery gene
  NG743_RS26260 (NG743_26130) pilT 5758268..5759368 (+) 1101 WP_053539894.1 type IV pilus twitching motility protein PilT Machinery gene
  NG743_RS26265 (NG743_26135) - 5759445..5760668 (+) 1224 WP_027401135.1 type II secretion system F family protein -
  NG743_RS26270 (NG743_26140) - 5760758..5761318 (+) 561 WP_257121197.1 hypothetical protein -
  NG743_RS26275 (NG743_26145) - 5761417..5762955 (+) 1539 WP_051424227.1 thymidylate synthase -

Sequence

Protein


Download         Length: 666 a.a.        Molecular weight: 74748.68 Da        Isoelectric Point: 5.3492

>NTDB_id=699832 NG743_RS26255 WP_027401137.1 5756203..5758203(+) (pilB/pilB1) [Dolichospermum heterosporum TAC447 strain NIES-1697]
MTYSSPQGRSTAITIRTEFSPFANKLVASGYINNEQMRQAVATSRKTGKLLTEVLESITDRKLAPEQVTAYKKQHLFELK
ILYGIESLDTEINEIDTTNVGMLIQELIPVELCRRHYLVPLSRHDDQPSPYILMAMVDPDNLEASDDLNRILRPKGLSFQ
RRVITQKDYLGIINQYLNEQAARQKTLDQQKNYDVKGDLDNLENLEIEESSDDDVDLDKAMKGADDAPIIKLVNRILLKA
LSEGVSDIHIEPQEEYLRIRFRKDGVLREAFENMPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRIFAGRKVDFRVNT
LPSRYGEKVVLRILDNSSTQLGLDKLITDPETLKIVQDMVSKPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVED
PIEYSLPGITQVQVIREKGLDFSTALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGEM
GIEPFMVSSSLIGVLAQRLVRRVCSECRIAYSPTIEELARYGLSASQEAQVTFYKANIIPPEEIVSRKNLCSRCNGIGYK
GRCGVYEVMRITEELQTLINKEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKRKSGL
TCRTCTGELQPEWLDCPYCMTPRFTD

Nucleotide


Download         Length: 2001 bp        

>NTDB_id=699832 NG743_RS26255 WP_027401137.1 5756203..5758203(+) (pilB/pilB1) [Dolichospermum heterosporum TAC447 strain NIES-1697]
ATGACTTACTCATCACCACAAGGGCGCAGTACGGCGATAACTATCAGAACTGAGTTTTCGCCCTTTGCTAATAAACTAGT
AGCATCTGGCTATATCAATAATGAACAAATGAGACAGGCTGTAGCTACGAGCCGCAAGACTGGCAAGCTACTGACAGAAG
TATTAGAGTCAATTACTGACCGAAAATTAGCTCCTGAACAGGTAACAGCATACAAAAAACAACACTTATTTGAACTTAAA
ATTTTATACGGTATTGAGTCGCTTGATACTGAAATCAACGAAATTGATACAACAAATGTGGGGATGCTGATTCAAGAACT
CATTCCTGTGGAGCTATGTCGTCGCCATTATCTAGTGCCATTGTCAAGACATGATGACCAACCGTCACCTTATATTTTAA
TGGCGATGGTTGACCCAGATAATTTAGAGGCTTCTGATGATCTTAACCGAATTTTGCGTCCTAAAGGTTTGTCTTTTCAG
CGGCGGGTAATTACGCAGAAAGATTACTTGGGCATCATTAATCAATACTTAAATGAGCAAGCTGCCCGCCAAAAGACCTT
AGATCAACAAAAAAATTATGATGTTAAGGGGGATTTAGACAATTTAGAGAATCTGGAAATAGAGGAAAGTAGTGATGATG
ATGTTGATTTAGATAAGGCGATGAAGGGTGCTGATGATGCACCTATTATTAAACTTGTGAATCGGATTTTGTTGAAGGCT
TTAAGTGAGGGTGTTTCGGATATTCACATTGAACCCCAGGAAGAATATTTACGGATTCGTTTCCGTAAGGATGGGGTATT
ACGTGAGGCATTTGAAAATATGCCGAAAAAAATTATCCCGGCAGTAACAGCCAGATTTAAAATTATTTCCAATTTGGACA
TTGCCGAGAGACGTTTACCTCAAGATGGACGTATCCGCCGGATATTTGCGGGGAGAAAGGTGGATTTTCGGGTAAATACT
CTACCTAGTCGTTATGGAGAAAAGGTTGTTCTCCGGATTTTAGATAACTCCTCTACCCAATTGGGTTTAGATAAGTTAAT
TACTGACCCAGAAACCTTAAAAATTGTTCAGGATATGGTTAGTAAGCCCTTTGGGTTGATTTTGGTGACAGGACCAACTG
GTTCGGGTAAAACGACTTCGTTGTATTCGGCACTGTCAGAAAAGAATGATCCGGGGATTAATATTAGTACGGTAGAAGAT
CCGATTGAGTACAGTTTACCGGGGATTACTCAAGTACAGGTAATTCGGGAAAAGGGGTTGGATTTCTCGACGGCTTTGCG
GGCTTTCTTGCGTCAAGATCCAGATGTGTTGCTGGTGGGGGAAACACGAGATAAGGAAACTGCAAAAACGGCGATTGAGG
CGGCATTAACCGGTCACTTGGTATTAACTACTTTACACACTAATGATGCGCCGGGAGCGATCGCTCGCTTAGGAGAAATG
GGTATTGAACCGTTCATGGTTTCCAGTTCTCTCATTGGTGTTTTAGCACAACGTCTAGTTCGTCGTGTCTGTTCTGAATG
TCGGATTGCCTATAGTCCCACCATTGAAGAACTCGCTCGCTATGGTTTATCAGCTTCCCAAGAGGCTCAGGTGACTTTCT
ATAAAGCCAATATTATCCCACCAGAAGAAATAGTCAGCCGTAAAAATCTTTGTTCAAGATGTAATGGCATTGGCTACAAG
GGGCGTTGTGGTGTTTATGAAGTGATGCGGATTACTGAAGAATTACAAACCCTTATTAACAAGGAAGCACCTACAGAAAG
GATCAAAGAAGTGGCTGTTGAAGAGGGTATGAAAACCTTACTGGCATACAGTTTAGACCTAGTTCGCCAAGGTGCAACTA
CCCTAGAAGAGGTAGAACGGGTAACATTTACAGACACAGGTTTAGAAGCTGAATTAAAGGCTAAACGCAAAAGTGGTCTG
ACTTGTCGGACTTGTACAGGGGAATTACAACCAGAATGGTTAGATTGTCCTTACTGTATGACACCTCGATTTACAGATTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(235-502)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

63.596

100

0.643