Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFL62_RS19110 Genome accession   NZ_CP099327
Coordinates   3988117..3989142 (-) Length   341 a.a.
NCBI ID   WP_073396410.1    Uniprot ID   -
Organism   Enterobacter ludwigii strain RHB47-SO-C03     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3983117..3994142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFL62_RS19080 (NFL62_19075) - 3984018..3984515 (+) 498 WP_032670169.1 SprT family zinc-dependent metalloprotease -
  NFL62_RS19085 (NFL62_19080) endA 3984610..3985317 (+) 708 WP_020883698.1 deoxyribonuclease I -
  NFL62_RS19090 (NFL62_19085) rsmE 3985370..3986101 (+) 732 WP_020883697.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFL62_RS19095 (NFL62_19090) gshB 3986141..3987088 (+) 948 WP_279257840.1 glutathione synthase -
  NFL62_RS19100 (NFL62_19095) - 3987175..3987735 (+) 561 WP_020883695.1 YqgE/AlgH family protein -
  NFL62_RS19105 (NFL62_19100) ruvX 3987735..3988151 (+) 417 WP_014171567.1 Holliday junction resolvase RuvX -
  NFL62_RS19110 (NFL62_19105) pilT 3988117..3989142 (-) 1026 WP_073396410.1 type IV pilus twitching motility protein PilT Machinery gene
  NFL62_RS19115 (NFL62_19110) - 3989160..3989861 (+) 702 WP_073396409.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFL62_RS19120 (NFL62_19115) - 3989883..3990449 (+) 567 WP_014171570.1 YggT family protein -
  NFL62_RS19125 (NFL62_19120) yggU 3990446..3990742 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  NFL62_RS19130 (NFL62_19125) - 3990746..3991339 (+) 594 WP_040018848.1 XTP/dITP diphosphatase -
  NFL62_RS19135 (NFL62_19130) hemW 3991332..3992474 (+) 1143 WP_063858432.1 radical SAM family heme chaperone HemW -
  NFL62_RS19140 (NFL62_19135) - 3992538..3992900 (+) 363 WP_138614617.1 DUF559 domain-containing protein -
  NFL62_RS19145 (NFL62_19140) - 3992962..3993678 (-) 717 WP_279257841.1 DUF2884 domain-containing protein -
  NFL62_RS19150 (NFL62_19145) - 3993735..3994061 (-) 327 WP_020883688.1 YggL family protein -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37131.20 Da        Isoelectric Point: 6.0946

>NTDB_id=698805 NFL62_RS19110 WP_073396410.1 3988117..3989142(-) (pilT) [Enterobacter ludwigii strain RHB47-SO-C03]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRAGRLEPAPFPPPEVDTLLKAWLNDEQQGTWWANGQVDFAVTLAGNQRLRG
SVFKQTSGVSMTLRLLPGVCPQLSALGVPRVVPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FLYQSERCLIQQREIGQHSPSFADALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDAQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGQLQAPALNNPVRNSFRE

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=698805 NFL62_RS19110 WP_073396410.1 3988117..3989142(-) (pilT) [Enterobacter ludwigii strain RHB47-SO-C03]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGAGCAGGTCGTCTTGAACCCGCGCCGTTTCCGCCTCCCGAAGTCGATACGCTATTAAAGGCGTGGCTCAACG
ATGAACAGCAGGGAACGTGGTGGGCGAACGGGCAGGTGGATTTTGCCGTTACCCTGGCGGGCAATCAGCGCCTGCGCGGA
AGCGTGTTTAAACAGACAAGCGGCGTCTCAATGACGCTGCGGCTGTTGCCCGGGGTCTGCCCGCAACTCTCCGCGTTGGG
GGTGCCACGGGTTGTTCCTGAGCTGTTATCCAGTGACAACGGGTTGATCCTGGTCACCGGGGCAACCGGCAGCGGTAAGT
CCACTACGCTGGCAGCGATGGTGGATTTTCTTAATCACCATACTGACGGGCATATTCTGACGCTCGAAGACCCGGTGGAG
TTTCTGTACCAGAGCGAACGGTGCCTGATCCAGCAGCGCGAGATTGGCCAACACAGCCCGTCATTTGCCGACGCGCTGCG
CGGGGCGTTACGCCAGGATCCTGATGTCATACTGCTGGGTGAGCTGCGCGACAGTGAAACAATCCGCCTCGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTCGCGACACTGCATACCCGCGGGGCAGCGCAGGCGATTGAGCGGCTGGTCGATACG
TTTCCGGCGCAGGAGAAAGATCCGGTGCGCAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTCCTCCC
CGATGCGCAGGGCGGGCGCGTGGCGCTTTATGAGCTGCTGGTGAATACTCCGGCGGCGGCGAATCTCATTCGCGAAGGAA
AAACGTGGCAATTGCCCGGTATTATTCAAACCGGCCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCTGAAAGA
CGGGCGCAGGGGCAACTCCAGGCCCCGGCGCTCAATAACCCTGTTCGAAATAGCTTTCGAGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.817

99.12

0.484
  pilT Vibrio cholerae strain A1552

48.817

99.12

0.484
  pilT Neisseria meningitidis 8013

48.048

97.654

0.469
  pilT Neisseria gonorrhoeae MS11

46.491

100

0.466
  pilT Legionella pneumophila strain ERS1305867

48.758

94.428

0.46
  pilT Legionella pneumophila strain Lp02

48.758

94.428

0.46
  pilT Acinetobacter baylyi ADP1

47.289

97.361

0.46
  pilT Pseudomonas stutzeri DSM 10701

47.401

95.894

0.455
  pilT Acinetobacter baumannii strain A118

47.095

95.894

0.452
  pilT Acinetobacter baumannii D1279779

47.095

95.894

0.452
  pilT Pseudomonas aeruginosa PAK

47.095

95.894

0.452
  pilT Acinetobacter nosocomialis M2

47.5

93.842

0.446
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.099

94.428

0.416
  pilU Pseudomonas stutzeri DSM 10701

40

96.774

0.387
  pilU Vibrio cholerae strain A1552

39.13

94.428

0.369