Detailed information NTG0698805

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: PCP89_RS09615
• Genome accession: NZ_CP115249
• Coordinates: 2066223..2066696 (-)
• Length: 157 a.a.
• NCBI ID: WP_015503553.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain F037
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2061223..2071696

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP89_RS09580 (PCP89_09575)	-	2061666..2062328 (+)	663	WP_033992423.1	DUF3218 family protein	-
PCP89_RS09585 (PCP89_09580)	fliP	2062608..2063363 (+)	756	WP_031285507.1	flagellar type III secretion system pore protein FliP	-
PCP89_RS09590 (PCP89_09585)	fliQ	2063408..2063677 (+)	270	WP_023435365.1	flagellar biosynthesis protein FliQ	-
PCP89_RS09595 (PCP89_09590)	fliR	2063677..2064459 (+)	783	Protein_1899	flagellar biosynthetic protein FliR	-
PCP89_RS09600 (PCP89_09595)	-	2064462..2064721 (+)	260	Protein_1900	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
PCP89_RS09605 (PCP89_09600)	-	2065750..2065992 (+)	243	WP_023082027.1	hypothetical protein	-
PCP89_RS09615 (PCP89_09610)	pilE	2066223..2066696 (-)	474	WP_015503553.1	pilin	Machinery gene
PCP89_RS09620 (PCP89_09615)	pilB	2066927..2068627 (+)	1701	WP_003120945.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PCP89_RS09625 (PCP89_09620)	pilC	2068631..2069848 (+)	1218	WP_010792214.1	type II secretion system F family protein	Machinery gene
PCP89_RS09630 (PCP89_09625)	pilD	2069851..2070723 (+)	873	WP_003116251.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PCP89_RS09635 (PCP89_09630)	coaE	2070720..2071331 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
PCP89_RS09640 (PCP89_09635)	yacG	2071328..2071528 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 157 a.a.
• Molecular weight: 16090.45 Da
• Isoelectric Point: 8.4684

>NTDB_id=698805 PCP89_RS09615 WP_015503553.1 2066223..2066696(-) (pilE) [Pseudomonas aeruginosa strain F037]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKASDVATPLRGKTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide

• Length: 474 bp

>NTDB_id=698805 PCP89_RS09615 WP_015503553.1 2066223..2066696(-) (pilE) [Pseudomonas aeruginosa strain F037]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGCCTCTGATGTGGCTACTCCTCTGAGGGGGAAAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA

Domains

Predicted by InterProScan.

• Pilin (bacterial filament) (Pilin) (36-150)

• Prokaryotic N-terminal methylation motif (N_methyl) (4-27)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Neisseria gonorrhoeae strain FA1090	42.197	100	0.465
comP	Acinetobacter baylyi ADP1	47.059	97.452	0.459
pilE	Neisseria gonorrhoeae MS11	42.515	100	0.452
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	41.176	100	0.446
pilA/pilA1	Eikenella corrodens VA1	41.975	100	0.433
pilA2	Legionella pneumophila str. Paris	45.578	93.631	0.427
pilA2	Legionella pneumophila strain ERS1305867	44.218	93.631	0.414
pilA	Ralstonia pseudosolanacearum GMI1000	39.394	100	0.414
pilA/pilAI	Pseudomonas stutzeri DSM 10701	43.333	95.541	0.414
pilA	Pseudomonas aeruginosa PAK	41.722	96.178	0.401
pilA	Acinetobacter nosocomialis M2	55.357	71.338	0.395