Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFK27_RS18375 Genome accession   NZ_CP099309
Coordinates   3881094..3882119 (-) Length   341 a.a.
NCBI ID   WP_279259847.1    Uniprot ID   -
Organism   Enterobacter ludwigii strain RHB25-E3-C07     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3876094..3887119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFK27_RS18345 (NFK27_18340) - 3876995..3877492 (+) 498 WP_032670169.1 SprT family zinc-dependent metalloprotease -
  NFK27_RS18350 (NFK27_18345) endA 3877587..3878294 (+) 708 WP_279259846.1 deoxyribonuclease I -
  NFK27_RS18355 (NFK27_18350) rsmE 3878347..3879078 (+) 732 WP_020883697.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFK27_RS18360 (NFK27_18355) gshB 3879118..3880065 (+) 948 WP_020883696.1 glutathione synthase -
  NFK27_RS18365 (NFK27_18360) - 3880152..3880712 (+) 561 WP_020883695.1 YqgE/AlgH family protein -
  NFK27_RS18370 (NFK27_18365) ruvX 3880712..3881128 (+) 417 WP_014171567.1 Holliday junction resolvase RuvX -
  NFK27_RS18375 (NFK27_18370) pilT 3881094..3882119 (-) 1026 WP_279259847.1 type IV pilus twitching motility protein PilT Machinery gene
  NFK27_RS18380 (NFK27_18375) - 3882137..3882838 (+) 702 WP_214580506.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFK27_RS18385 (NFK27_18380) - 3882860..3883426 (+) 567 WP_014171570.1 YggT family protein -
  NFK27_RS18390 (NFK27_18385) yggU 3883423..3883719 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  NFK27_RS18395 (NFK27_18390) - 3883723..3884316 (+) 594 WP_047956088.1 XTP/dITP diphosphatase -
  NFK27_RS18400 (NFK27_18395) hemW 3884309..3885451 (+) 1143 WP_014171573.1 radical SAM family heme chaperone HemW -
  NFK27_RS18405 (NFK27_18400) - 3885515..3885877 (+) 363 WP_059306207.1 endonuclease domain-containing protein -
  NFK27_RS18410 (NFK27_18405) - 3885939..3886655 (-) 717 WP_020883689.1 DUF2884 domain-containing protein -
  NFK27_RS18415 (NFK27_18410) - 3886712..3887038 (-) 327 WP_032678951.1 YggL family protein -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37119.14 Da        Isoelectric Point: 6.0946

>NTDB_id=698624 NFK27_RS18375 WP_279259847.1 3881094..3882119(-) (pilT) [Enterobacter ludwigii strain RHB25-E3-C07]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPEVDTLLKAWLNDEQQGTWWANGQVDFAVTLAGNQRLRG
SAFKQTSGVSMTLRLLPGVCPQLPALGVPRVVPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FLYQSERCLIQQREIGQHSPSFADALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FSAQEKDPVRNQLAGSLRAVLAQKLLPDAQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGQLQAPALNNPVRNSFRE

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=698624 NFK27_RS18375 WP_279259847.1 3881094..3882119(-) (pilT) [Enterobacter ludwigii strain RHB25-E3-C07]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATCAGGTCGTCTTGAACCCGCACCGTTTCCGCCGCCCGAAGTCGATACGCTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGAACGTGGTGGGCGAACGGGCAGGTGGATTTTGCCGTCACCCTGGCGGGCAACCAGCGCCTGCGCGGA
AGCGCGTTTAAACAGACAAGCGGCGTCTCAATGACGCTGCGGCTGTTGCCCGGGGTCTGCCCGCAACTCCCCGCGTTGGG
GGTGCCCCGGGTTGTTCCTGAGCTGTTATCCAGTGACAACGGGTTGATCCTGGTCACCGGGGCAACCGGCAGCGGTAAGT
CCACTACGCTGGCAGCGATGGTAGATTTTCTTAATCACCATACTGACGGGCATATTCTGACGCTCGAAGACCCGGTGGAG
TTTCTGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGCGAGATTGGCCAACACAGCCCGTCATTTGCCGACGCGCTGCG
CGGGGCGTTACGCCAGGATCCTGATGTCATACTGCTGGGTGAGCTGCGCGACAGTGAAACGATCCGCCTCGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTCGCGACACTGCATACCCGCGGGGCAGCGCAGGCGATTGAGCGGCTGGTCGATACG
TTTTCGGCGCAGGAGAAAGATCCGGTGCGCAATCAGCTGGCCGGCAGCCTGCGGGCAGTGCTGGCGCAAAAGCTCCTCCC
CGATGCGCAGGGCGGGCGCGTGGCGCTTTATGAGCTGCTGGTGAATACTCCGGCGGCGGCGAATCTCATTCGCGAAGGAA
AAACGTGGCAATTGCCCGGTATTATTCAAACCGGCCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCTGAAAGA
CGGGCGCAGGGGCAACTCCAGGCCCCGGCGCTCAATAACCCTGTTCGAAATAGCTTTCGAGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.817

99.12

0.484
  pilT Vibrio cholerae strain A1552

48.817

99.12

0.484
  pilT Neisseria meningitidis 8013

48.048

97.654

0.469
  pilT Neisseria gonorrhoeae MS11

46.491

100

0.466
  pilT Legionella pneumophila strain ERS1305867

48.758

94.428

0.46
  pilT Legionella pneumophila strain Lp02

48.758

94.428

0.46
  pilT Pseudomonas stutzeri DSM 10701

47.401

95.894

0.455
  pilT Acinetobacter baylyi ADP1

46.687

97.361

0.455
  pilT Acinetobacter baumannii D1279779

47.095

95.894

0.452
  pilT Pseudomonas aeruginosa PAK

47.095

95.894

0.452
  pilT Acinetobacter baumannii strain A118

47.095

95.894

0.452
  pilT Acinetobacter nosocomialis M2

47.5

93.842

0.446
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.41

94.428

0.419
  pilU Pseudomonas stutzeri DSM 10701

40

96.774

0.387
  pilU Vibrio cholerae strain A1552

39.13

94.428

0.369