Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M9782_RS19640 Genome accession   NZ_CP097896
Coordinates   4464052..4465068 (-) Length   338 a.a.
NCBI ID   WP_275071175.1    Uniprot ID   -
Organism   Pectobacterium actinidiae strain GX-Pa1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4459052..4470068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9782_RS19625 (M9782_19610) nsrR 4459269..4459694 (-) 426 WP_039361343.1 nitric oxide-sensing transcriptional repressor NsrR -
  M9782_RS19630 (M9782_19615) - 4460039..4461610 (+) 1572 WP_039361345.1 methyl-accepting chemotaxis protein -
  M9782_RS19635 (M9782_19620) - 4462257..4464083 (+) 1827 WP_039361347.1 sensor domain-containing diguanylate cyclase -
  M9782_RS19640 (M9782_19625) pilT 4464052..4465068 (-) 1017 WP_275071175.1 type IV pilus twitching motility protein PilT Machinery gene
  M9782_RS19645 (M9782_19630) - 4465090..4465803 (+) 714 WP_275071176.1 YggS family pyridoxal phosphate-dependent enzyme -
  M9782_RS19650 (M9782_19635) proC 4465900..4466721 (+) 822 WP_275072433.1 pyrroline-5-carboxylate reductase -
  M9782_RS19655 (M9782_19640) - 4466771..4467325 (+) 555 WP_010295003.1 YggT family protein -
  M9782_RS19660 (M9782_19645) yggU 4467322..4467612 (+) 291 WP_010295005.1 DUF167 family protein YggU -
  M9782_RS19665 (M9782_19650) - 4467644..4468237 (+) 594 WP_275071177.1 XTP/dITP diphosphatase -
  M9782_RS19670 (M9782_19655) hemW 4468230..4469372 (+) 1143 WP_275071178.1 radical SAM family heme chaperone HemW -
  M9782_RS19675 (M9782_19660) - 4469598..4469861 (+) 264 WP_226293727.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36712.04 Da        Isoelectric Point: 6.8157

>NTDB_id=693463 M9782_RS19640 WP_275071175.1 4464052..4465068(-) (pilT) [Pectobacterium actinidiae strain GX-Pa1]
MELDEWIARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPDQVAQWCDAWLEPAQSEQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSVQPSLDALHAPLVFSTLLEKPNGLILITGATGSGKSTTLAAMLGKLNDSCDRHVITLE
DPIEFLHASRRCLIQQREIGAHSVSFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSAAQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLSAARGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCQDGLISRSCALAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=693463 M9782_RS19640 WP_275071175.1 4464052..4465068(-) (pilT) [Pectobacterium actinidiae strain GX-Pa1]
ATGGAATTGGATGAGTGGATAGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGCCACCCGCCGGT
GTTGCGCGTGGATGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCAGATCAGGTGGCGCAATGGTGCGATG
CCTGGCTCGAACCGGCTCAGAGCGAACAACTTCGGCAAGCCGGTCAGGTGGATGGGGCATTAATGTTGCCCGATGGACAG
CGCCTGCGGGTGAATGTGTTTCGCCAGCGTGAGGGGCTGTCTGCGGCGCTGCGCGTCATCCCGTCCGTTCAACCGTCATT
GGATGCATTACATGCGCCACTCGTTTTCTCGACGCTGCTGGAAAAACCGAATGGACTGATCCTGATTACCGGTGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCAATGCTTGGGAAGTTAAACGACAGCTGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTCTCCATGCCAGCCGACGCTGTTTGATTCAACAGCGGGAGATCGGTGCGCACAGCGTGTCGTTTGC
GCAGGCACTACGAGCCGCATTGCGGGAAGATCCCGATGTCATTCTGCTGGGAGAACTGCGCGATACGGAGACAATTCGGC
TGGCGCTGACGGCGGCAGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACACGTAGCGCGGCGCAGGCAGTCGATCGT
CTAATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCTACCTGTTTGCAGGCGGTGGTGACGCA
AAAACTGTTATCTGCCGCGCGAGGGGGACGAATCGCGCTTTATGAAGTACTGACCGCCACTGCGGCAGTCAGCAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTCCCCGGCCTGATTCAGACGGGAGCCGCTGCGGGAATGCAAACGTTTGAGCAAAGC
TACCAGCAGCGCTGTCAGGATGGGCTTATTTCACGCAGTTGCGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.121

97.633

0.509
  pilT Vibrio cholerae strain A1552

52.121

97.633

0.509
  pilT Neisseria meningitidis 8013

50

99.408

0.497
  pilT Neisseria gonorrhoeae MS11

49.702

99.408

0.494
  pilT Pseudomonas stutzeri DSM 10701

49.552

99.112

0.491
  pilT Acinetobacter baylyi ADP1

49.112

100

0.491
  pilT Pseudomonas aeruginosa PAK

49.254

99.112

0.488
  pilT Acinetobacter nosocomialis M2

48.657

99.112

0.482
  pilT Acinetobacter baumannii strain A118

48.657

99.112

0.482
  pilT Acinetobacter baumannii D1279779

48.657

99.112

0.482
  pilT Legionella pneumophila strain Lp02

48.358

99.112

0.479
  pilT Legionella pneumophila strain ERS1305867

48.358

99.112

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.947

94.379

0.405
  pilU Vibrio cholerae strain A1552

38.872

99.704

0.388
  pilU Pseudomonas stutzeri DSM 10701

38.576

99.704

0.385