Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5J74_RS09005 Genome accession   NZ_CP097480
Coordinates   1912481..1913605 (-) Length   374 a.a.
NCBI ID   WP_015153786.1    Uniprot ID   K9TXX1
Organism   Chroococcidiopsis sp. CCNUC1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1907481..1918605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5J74_RS08985 (M5J74_08985) - 1908670..1909416 (-) 747 WP_250016336.1 isoprenyl transferase -
  M5J74_RS08990 (M5J74_08990) - 1909830..1910438 (+) 609 WP_250016338.1 GNAT family N-acetyltransferase -
  M5J74_RS08995 (M5J74_08995) - 1910526..1911101 (-) 576 WP_250016340.1 hypothetical protein -
  M5J74_RS09000 (M5J74_09000) - 1911166..1912395 (-) 1230 WP_015153785.1 type II secretion system F family protein -
  M5J74_RS09005 (M5J74_09005) pilT 1912481..1913605 (-) 1125 WP_015153786.1 type IV pilus twitching motility protein PilT Machinery gene
  M5J74_RS09010 (M5J74_09010) pilB/pilB1 1913791..1915800 (-) 2010 WP_015153787.1 GspE/PulE family protein Machinery gene
  M5J74_RS09015 (M5J74_09015) grpE 1916095..1916859 (+) 765 WP_015153788.1 nucleotide exchange factor GrpE -
  M5J74_RS09020 (M5J74_09020) - 1916860..1917009 (+) 150 WP_158265874.1 hypothetical protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41011.05 Da        Isoelectric Point: 6.6134

>NTDB_id=689691 M5J74_RS09005 WP_015153786.1 1912481..1913605(-) (pilT) [Chroococcidiopsis sp. CCNUC1]
MELMIEDLMEEVINSGGSDLHISAGLPPYIRISGKLTPTEHEPLTAEQCQRLIFSMLNNTQRKTLEQNWELDCSYGIKGL
ARFRVNVYKDRGTYATCLRALASKIPSMETLNLPSIVREISEKPRGLVLVTGPTGSGKSTTLASMINNINTTRAEHIITV
EDPIEFVYEPIKSVIHQRQVGEDTKSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQQQIRVQLSNSLVAVFSQTLVSKKNPKPGEYGRVMAQEIMIVTPAISNLIREGKTAQIYSAIQTGGNLSM
QTLEKVLADQYKTGLISFEAAMSKTSKPDELQRLIGNTPGVGAKAGMRAGTSMH

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=689691 M5J74_RS09005 WP_015153786.1 1912481..1913605(-) (pilT) [Chroococcidiopsis sp. CCNUC1]
ATGGAATTAATGATTGAAGACTTAATGGAAGAAGTTATTAACTCGGGTGGTTCGGACTTACACATTTCAGCAGGTTTACC
GCCATATATCCGTATCAGTGGAAAGTTGACTCCAACGGAACACGAACCACTAACAGCAGAACAGTGTCAAAGACTAATTT
TTAGTATGTTAAATAATACCCAGCGCAAAACTCTAGAACAAAACTGGGAACTTGACTGTTCTTACGGGATTAAGGGACTA
GCTCGCTTCCGCGTCAACGTCTATAAAGACCGAGGTACGTATGCCACCTGCTTGCGAGCGCTAGCCTCCAAAATTCCCAG
CATGGAAACCTTAAATTTACCCAGTATTGTGCGGGAAATCTCGGAAAAACCACGAGGATTAGTCTTGGTAACGGGACCAA
CTGGATCGGGTAAGTCTACTACCCTAGCGTCCATGATTAATAATATTAATACAACGCGGGCGGAACACATTATCACAGTT
GAAGATCCGATTGAGTTTGTCTACGAACCAATCAAGAGTGTGATCCATCAGCGTCAAGTTGGAGAAGATACAAAAAGTTT
TGCTAATGCTCTTAGAGCAGCTTTACGGGAAGATCCAGATGTAATTCTGGTAGGCGAAATGCGTGACCTAGAAACAATTC
AGTTAGCGATTTCAGCAGCGGAAACAGGACACTTAGTATTTGGTACACTACACACGAGTTCTGCGGCTCAAACTGTTGAC
CGGATGGTAGATGTATTTCCTCCAGAACAACAGCAACAAATTCGCGTCCAGTTATCTAACTCACTTGTGGCAGTATTCAG
TCAAACTCTCGTATCTAAAAAGAACCCTAAACCAGGCGAATACGGTCGAGTTATGGCTCAGGAAATTATGATTGTTACCC
CTGCTATCTCTAACTTAATTCGTGAAGGAAAAACAGCTCAAATTTACTCGGCAATTCAAACTGGAGGAAATTTGAGTATG
CAAACATTGGAAAAAGTTTTAGCAGATCAGTATAAAACTGGTCTAATTTCGTTTGAAGCAGCAATGTCTAAAACATCTAA
GCCAGATGAATTACAGCGCTTGATTGGTAATACTCCAGGAGTCGGTGCAAAAGCTGGTATGAGAGCAGGTACTTCTATGC
ATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9TXX1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.89

92.781

0.5
  pilT Pseudomonas stutzeri DSM 10701

53.026

92.781

0.492
  pilT Legionella pneumophila strain Lp02

53.043

92.246

0.489
  pilT Legionella pneumophila strain ERS1305867

53.043

92.246

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.593

98.663

0.489
  pilT Acinetobacter baumannii strain A118

51.585

92.781

0.479
  pilT Acinetobacter baylyi ADP1

51.585

92.781

0.479
  pilT Acinetobacter baumannii D1279779

51.585

92.781

0.479
  pilT Acinetobacter nosocomialis M2

51.585

92.781

0.479
  pilT Neisseria gonorrhoeae MS11

50.143

93.316

0.468
  pilT Neisseria meningitidis 8013

50.143

93.316

0.468
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.706

90.909

0.452
  pilT Vibrio cholerae strain A1552

49.706

90.909

0.452
  pilU Acinetobacter baylyi ADP1

39.542

93.316

0.369