Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   M5J74_RS09010 Genome accession   NZ_CP097480
Coordinates   1913791..1915800 (-) Length   669 a.a.
NCBI ID   WP_015153787.1    Uniprot ID   K9TZ08
Organism   Chroococcidiopsis sp. CCNUC1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1908791..1920800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5J74_RS08990 (M5J74_08990) - 1909830..1910438 (+) 609 WP_250016338.1 GNAT family N-acetyltransferase -
  M5J74_RS08995 (M5J74_08995) - 1910526..1911101 (-) 576 WP_250016340.1 hypothetical protein -
  M5J74_RS09000 (M5J74_09000) - 1911166..1912395 (-) 1230 WP_015153785.1 type II secretion system F family protein -
  M5J74_RS09005 (M5J74_09005) pilT 1912481..1913605 (-) 1125 WP_015153786.1 type IV pilus twitching motility protein PilT Machinery gene
  M5J74_RS09010 (M5J74_09010) pilB/pilB1 1913791..1915800 (-) 2010 WP_015153787.1 GspE/PulE family protein Machinery gene
  M5J74_RS09015 (M5J74_09015) grpE 1916095..1916859 (+) 765 WP_015153788.1 nucleotide exchange factor GrpE -
  M5J74_RS09020 (M5J74_09020) - 1916860..1917009 (+) 150 WP_158265874.1 hypothetical protein -
  M5J74_RS09025 (M5J74_09025) dnaK 1916987..1918900 (+) 1914 WP_106217497.1 molecular chaperone DnaK -
  M5J74_RS09030 (M5J74_09030) dnaJ 1919131..1920264 (+) 1134 WP_192154253.1 molecular chaperone DnaJ -
  M5J74_RS09035 (M5J74_09035) - 1920261..1920512 (+) 252 WP_106544895.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 669 a.a.        Molecular weight: 74598.36 Da        Isoelectric Point: 4.8932

>NTDB_id=689692 M5J74_RS09010 WP_015153787.1 1913791..1915800(-) (pilB/pilB1) [Chroococcidiopsis sp. CCNUC1]
MSYSSSMQRRALIVKNDLSPFGNKVVETGYVSKEQMKQALSESRSTGRSLPEILEAITGRQLPPDLIRQYKKQQLFELKI
LYGVEFLDPEINQIGTSQVGELIDSLIPIDICRRYRLLPISKSDEPPSILIAMVDPDNLDAQDNLNRILRPRDIGLQRTV
ITLEDFQELLSKYLDENVEKQKQQADNQRNNANAVDFTVELDGLDLQDAPEEGDADLGASLKDADAAPVIALVNKILIKA
LQEKVSDIHIEPQEEYLRVRFRRDGVLRQAFDPLPKQIVPAVTARFKIIAELDIAERRAPQDGRIRRVFEGRKVDFRVNT
LPSRYGEKVVLRILDNSSTQLGLDKLISDVESLQIVRDLASRPFGLLLVTGPTGSGKSTTLYSILAERNDPGVNISTAED
PIEYSLPGITQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAISRLDEM
GVEPFMVSGALLGVVAQRLMRRVCSDCRIPYTPSADELARFGLASSKDVELTLYKAKSLELEEIQAAKAKNQLCSKCAGV
GYKGRCGVYEVMRVTERLQNLITEGAPSERIKEAAVEEGMKTLLAYSLELVMQGNTTLEEVERVTFTDSGLEAELKAKRK
SSLECKTCHAELQPEWFECPYCMTPRFSD

Nucleotide


Download         Length: 2010 bp        

>NTDB_id=689692 M5J74_RS09010 WP_015153787.1 1913791..1915800(-) (pilB/pilB1) [Chroococcidiopsis sp. CCNUC1]
ATGTCTTACTCCTCTTCAATGCAGCGGCGTGCCTTAATTGTCAAAAATGACTTGTCTCCTTTTGGCAACAAAGTAGTTGA
GACTGGGTATGTCAGTAAAGAGCAGATGAAGCAGGCTTTAAGCGAAAGTCGCTCGACTGGGCGATCGCTACCGGAGATTT
TAGAAGCTATAACTGGACGACAACTGCCACCAGACTTAATTAGACAATATAAAAAACAACAGCTATTCGAGCTAAAAATT
CTCTACGGTGTAGAGTTTCTCGATCCAGAAATCAATCAGATTGGCACAAGTCAAGTCGGCGAGTTGATCGATTCTTTGAT
CCCGATTGATATTTGTCGTCGCTATCGGCTGCTACCTATATCAAAAAGTGATGAGCCTCCGTCCATTTTGATCGCAATGG
TCGATCCAGATAATTTGGATGCCCAAGATAATTTAAACCGTATTCTTCGCCCCCGCGATATTGGATTGCAAAGGACGGTC
ATTACACTAGAAGACTTCCAAGAACTTCTTTCTAAATATTTAGATGAAAATGTAGAAAAACAAAAACAACAGGCAGACAA
TCAAAGAAATAACGCTAATGCCGTCGATTTCACTGTAGAGTTAGATGGACTTGATTTACAAGATGCACCAGAGGAGGGTG
ACGCAGATTTAGGTGCATCTCTCAAAGATGCGGATGCTGCTCCTGTAATTGCCCTAGTGAATAAAATTCTGATCAAAGCG
TTACAAGAAAAAGTTTCAGATATTCACATTGAACCCCAAGAAGAGTACTTGCGCGTCCGATTTCGTAGAGATGGAGTATT
GCGGCAGGCTTTTGACCCCCTACCCAAACAAATTGTTCCTGCTGTGACAGCTCGATTTAAAATTATTGCTGAATTAGATA
TTGCCGAACGACGCGCACCCCAAGATGGTCGTATTCGCCGCGTATTTGAAGGACGAAAAGTTGATTTTCGCGTCAATACC
TTACCCAGTCGTTACGGGGAAAAAGTTGTTTTGCGGATTTTGGATAACTCATCTACCCAACTTGGGTTAGACAAATTAAT
TAGCGATGTAGAGAGCCTGCAAATTGTGAGGGACTTGGCAAGTCGTCCGTTCGGACTGCTGTTGGTTACGGGTCCGACGG
GAAGCGGTAAGTCGACCACTCTATACTCAATTTTGGCAGAACGCAACGATCCAGGGGTAAATATCAGCACCGCTGAAGAC
CCCATTGAGTATTCTCTACCAGGAATTACGCAAGTCCAGGTGATTCGAGAAAAAGGAATGGATTTTGCGTCAATTTTGCG
GGCTTTTCTACGACAAGACCCAGACGTAATTCTAGTTGGTGAGACACGGGATAAGGAAACGGCAAAAACCGCTATTGAGG
CAGCACTGACGGGACACTTAGTATTGACAACTCTGCACACTAATGATGCGGCTGGGGCAATTTCTCGTTTAGACGAAATG
GGTGTAGAACCATTCATGGTGTCCGGTGCGCTGCTAGGGGTTGTGGCTCAACGTTTGATGCGGCGCGTCTGTTCTGATTG
TCGCATTCCTTATACTCCCAGTGCCGATGAGTTAGCTCGGTTTGGACTGGCGAGTTCTAAAGATGTCGAACTCACGCTTT
ACAAAGCTAAATCTTTGGAGTTAGAAGAAATTCAAGCCGCTAAGGCTAAAAATCAACTTTGCTCTAAATGCGCTGGCGTT
GGATATAAAGGGCGTTGTGGCGTATATGAAGTTATGCGCGTTACAGAACGACTACAAAATTTAATTACTGAAGGAGCGCC
GAGCGAACGGATTAAAGAAGCAGCCGTAGAAGAGGGCATGAAAACGTTGTTAGCTTATAGTCTGGAGCTAGTGATGCAAG
GTAACACGACTTTGGAAGAAGTAGAGCGCGTCACTTTCACTGATTCTGGTTTGGAAGCGGAACTGAAAGCAAAACGTAAA
AGTTCATTAGAGTGTAAAACTTGTCATGCAGAGCTACAACCAGAGTGGTTTGAGTGTCCTTATTGTATGACTCCACGATT
TAGTGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(235-502)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(27-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9TZ08

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

67.069

98.954

0.664