Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5K26_RS18840 Genome accession   NZ_CP097342
Coordinates   3930109..3931089 (-) Length   326 a.a.
NCBI ID   WP_045910774.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. xiangfangensis strain va18651     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3925109..3936089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5K26_RS18810 (M5K26_18785) - 3925987..3926484 (+) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -
  M5K26_RS18815 (M5K26_18790) endA 3926579..3927286 (+) 708 WP_003860034.1 deoxyribonuclease I -
  M5K26_RS18820 (M5K26_18795) rsmE 3927338..3928069 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5K26_RS18825 (M5K26_18800) gshB 3928101..3929048 (+) 948 WP_023295410.1 glutathione synthase -
  M5K26_RS18830 (M5K26_18805) - 3929123..3929683 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  M5K26_RS18835 (M5K26_18810) ruvX 3929683..3930099 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  M5K26_RS18840 (M5K26_18815) pilT 3930109..3931089 (-) 981 WP_045910774.1 type IV pilus twitching motility protein PilT Machinery gene
  M5K26_RS18845 (M5K26_18820) - 3931107..3931808 (+) 702 WP_045347340.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5K26_RS18850 (M5K26_18825) - 3931830..3932396 (+) 567 WP_015571838.1 YggT family protein -
  M5K26_RS18855 (M5K26_18830) yggU 3932393..3932689 (+) 297 WP_023304404.1 DUF167 family protein YggU -
  M5K26_RS18860 (M5K26_18835) - 3932693..3933286 (+) 594 WP_015571839.1 XTP/dITP diphosphatase -
  M5K26_RS18865 (M5K26_18840) hemW 3933279..3934427 (+) 1149 WP_045910775.1 radical SAM family heme chaperone HemW -
  M5K26_RS18870 (M5K26_18845) - 3934484..3934822 (+) 339 WP_045910776.1 endonuclease domain-containing protein -
  M5K26_RS18875 (M5K26_18850) - 3934904..3935620 (-) 717 WP_015571842.1 DUF2884 domain-containing protein -
  M5K26_RS18880 (M5K26_18855) - 3935677..3936003 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35736.71 Da        Isoelectric Point: 6.4131

>NTDB_id=688826 M5K26_RS18840 WP_045910774.1 3930109..3931089(-) (pilT) [Enterobacter hormaechei subsp. xiangfangensis strain va18651]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGATQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=688826 M5K26_RS18840 WP_045910774.1 3930109..3931089(-) (pilT) [Enterobacter hormaechei subsp. xiangfangensis strain va18651]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAGCTGCTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGTCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTACG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCACACGCGCGGTGCGACGCAGGCGATTGAGCGCCTGGTCGATACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374