Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M3M30_RS02750 Genome accession   NZ_CP097161
Coordinates   624154..625272 (-) Length   372 a.a.
NCBI ID   WP_218797746.1    Uniprot ID   -
Organism   Methylococcus capsulatus strain MIR     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 619154..630272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3M30_RS02730 (M3M30_02730) - 620459..621241 (+) 783 WP_010960814.1 succinate dehydrogenase iron-sulfur subunit -
  M3M30_RS02735 (M3M30_02735) - 621238..621489 (+) 252 WP_218800094.1 succinate dehydrogenase assembly factor 2 -
  M3M30_RS02740 (M3M30_02740) - 621483..621905 (+) 423 WP_017365686.1 protein YgfX -
  M3M30_RS02745 (M3M30_02745) - 621886..623709 (+) 1824 WP_218800093.1 VWA domain-containing protein -
  M3M30_RS02750 (M3M30_02750) pilU 624154..625272 (-) 1119 WP_218797746.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M3M30_RS02755 (M3M30_02755) pilT 625288..626322 (-) 1035 WP_010960809.1 type IV pilus twitching motility protein PilT Machinery gene
  M3M30_RS02760 (M3M30_02760) - 626378..627067 (+) 690 WP_010960808.1 YggS family pyridoxal phosphate-dependent enzyme -
  M3M30_RS02765 (M3M30_02765) proC 627138..627962 (+) 825 WP_010960807.1 pyrroline-5-carboxylate reductase -
  M3M30_RS02770 (M3M30_02770) - 627966..628544 (+) 579 WP_218800092.1 YggT family protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41667.00 Da        Isoelectric Point: 6.5905

>NTDB_id=687510 M3M30_RS02750 WP_218797746.1 624154..625272(-) (pilU) [Methylococcus capsulatus strain MIR]
MEFESLLKLMVLKKASDLFITAAKEPCMKLNGAIVPLSSTKLSTDQVRQLVLGIMNQRQRDEFENTNECNFALSAAGLGR
FRVSAFVQRNSPGMVLRRIETEIPTVEQLNLPSVLNDLVMTKRGLILFVGATGTGKSTSLAAMLKYRNEHSSGHIITIED
PLEFVHPHAGCIVTQREVGIDTESYEVALKNTLRQAPDVILIGEIRTRETMQQAITFAETGHLCLSTLHANNANQALDRI
LHFFPEDMHPQVFMDLSLNLRAIIAQQLVRRADGKGRYPAVEILINTPLVSDLIRQGEVHKLKDVMKQSREQGMQTFDQA
LFELFKAGRIGYEDALYSADSKNEVRLMIKLSEEGSIDKYAPKDDSIRIVDN

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=687510 M3M30_RS02750 WP_218797746.1 624154..625272(-) (pilU) [Methylococcus capsulatus strain MIR]
ATGGAATTCGAATCGCTGCTCAAGCTGATGGTACTGAAGAAAGCGTCCGATCTCTTCATCACCGCGGCCAAGGAGCCCTG
CATGAAGCTGAACGGCGCCATCGTGCCGCTGTCCAGCACCAAACTCTCCACCGACCAGGTGCGCCAGCTCGTCCTCGGGA
TCATGAACCAGCGCCAGCGCGACGAGTTCGAGAACACCAACGAATGCAACTTCGCCCTCTCCGCGGCCGGACTGGGCCGG
TTCCGGGTCAGCGCCTTCGTGCAGCGGAACAGTCCCGGCATGGTACTGCGCAGGATCGAGACCGAGATTCCCACCGTGGA
ACAGCTCAATCTGCCCTCCGTCCTCAATGACCTGGTCATGACCAAGCGCGGCCTGATCCTGTTCGTGGGCGCGACCGGCA
CCGGCAAGTCGACGTCGCTGGCGGCGATGCTCAAGTACCGCAACGAGCACTCCAGCGGCCACATCATCACCATCGAGGAT
CCCTTGGAATTCGTACACCCGCACGCGGGCTGCATCGTCACCCAGCGCGAGGTCGGCATCGATACCGAATCCTACGAAGT
CGCGCTGAAGAACACCCTGCGGCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGCACCCGCGAGACCATGCAGCAGG
CCATCACCTTCGCCGAAACCGGCCACCTCTGCCTGAGCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTCCATTTCTTCCCGGAAGACATGCATCCGCAGGTGTTCATGGACCTGTCGCTGAACCTGCGTGCCATCATCGCCCAGCA
GCTCGTCAGGCGCGCCGACGGCAAGGGGCGCTATCCGGCGGTGGAAATCCTCATCAATACCCCTCTGGTCTCCGACCTGA
TCCGCCAGGGCGAGGTCCACAAGCTCAAGGACGTGATGAAACAGTCGCGCGAACAGGGCATGCAGACCTTCGACCAGGCG
CTGTTCGAATTGTTCAAGGCGGGCAGGATCGGCTACGAGGATGCCCTCTACTCCGCCGATTCCAAGAACGAAGTCCGCTT
GATGATCAAGCTCAGCGAAGAAGGCAGCATCGACAAATACGCGCCCAAGGACGACTCGATCCGGATCGTCGACAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.774

95.161

0.664
  pilU Acinetobacter baylyi ADP1

64.706

95.968

0.621
  pilU Vibrio cholerae strain A1552

58.286

94.086

0.548
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.391

93.548

0.406
  pilT Legionella pneumophila strain ERS1305867

40.233

92.204

0.371
  pilT Legionella pneumophila strain Lp02

40.233

92.204

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

89.785

0.371
  pilT Vibrio cholerae strain A1552

41.317

89.785

0.371
  pilT Acinetobacter baylyi ADP1

39.883

91.667

0.366
  pilT Pseudomonas aeruginosa PAK

40.597

90.054

0.366
  pilT Pseudomonas stutzeri DSM 10701

40

90.054

0.36
  pilT Acinetobacter baumannii strain A118

39.528

91.129

0.36
  pilT Acinetobacter baumannii D1279779

39.528

91.129

0.36
  pilT Acinetobacter nosocomialis M2

39.528

91.129

0.36