Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M3M30_RS02755 Genome accession   NZ_CP097161
Coordinates   625288..626322 (-) Length   344 a.a.
NCBI ID   WP_010960809.1    Uniprot ID   Q608F5
Organism   Methylococcus capsulatus strain MIR     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 620288..631322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3M30_RS02730 (M3M30_02730) - 620459..621241 (+) 783 WP_010960814.1 succinate dehydrogenase iron-sulfur subunit -
  M3M30_RS02735 (M3M30_02735) - 621238..621489 (+) 252 WP_218800094.1 succinate dehydrogenase assembly factor 2 -
  M3M30_RS02740 (M3M30_02740) - 621483..621905 (+) 423 WP_017365686.1 protein YgfX -
  M3M30_RS02745 (M3M30_02745) - 621886..623709 (+) 1824 WP_218800093.1 VWA domain-containing protein -
  M3M30_RS02750 (M3M30_02750) pilU 624154..625272 (-) 1119 WP_218797746.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M3M30_RS02755 (M3M30_02755) pilT 625288..626322 (-) 1035 WP_010960809.1 type IV pilus twitching motility protein PilT Machinery gene
  M3M30_RS02760 (M3M30_02760) - 626378..627067 (+) 690 WP_010960808.1 YggS family pyridoxal phosphate-dependent enzyme -
  M3M30_RS02765 (M3M30_02765) proC 627138..627962 (+) 825 WP_010960807.1 pyrroline-5-carboxylate reductase -
  M3M30_RS02770 (M3M30_02770) - 627966..628544 (+) 579 WP_218800092.1 YggT family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38292.98 Da        Isoelectric Point: 6.7754

>NTDB_id=687511 M3M30_RS02755 WP_010960809.1 625288..626322(-) (pilT) [Methylococcus capsulatus strain MIR]
MDIAELLAFSVKNKSSDLHLSSGLPPMIRVDGDIRRINVPPLEHKEVHALIYDIMNDKQRRDFEEFLETDFSFELPGVAR
FRVNAFNQDRGAAAVFRTIPSKVLTLEELGCPKFFQDVTRQPRGLIVVTGPTGSGKSTTLAAMIDYINSNDYSHILTIED
PIEFVHQSKKCLINQREVHRNTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMIRSMLSESLQAVISQALVKKAGGGRTAAWEIMVGTPAIRNLIREAKVAQMYSTIQTGRKDGMQTLDQHL
QELVEKGIVTRQVAREVAVNKAAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=687511 M3M30_RS02755 WP_010960809.1 625288..626322(-) (pilT) [Methylococcus capsulatus strain MIR]
ATGGATATCGCCGAACTGCTGGCCTTCTCGGTCAAGAACAAATCCTCCGACCTGCATCTTTCCTCTGGGTTGCCGCCGAT
GATCCGCGTCGACGGCGACATCCGGCGCATCAACGTGCCTCCGCTGGAGCACAAGGAAGTGCACGCCCTCATCTATGACA
TCATGAACGACAAACAGCGCCGCGACTTCGAGGAATTTCTGGAAACCGACTTCTCGTTCGAGCTTCCGGGCGTCGCCCGT
TTCCGCGTCAACGCCTTCAACCAGGATCGGGGCGCGGCGGCCGTATTTCGCACCATTCCTTCCAAGGTCCTGACACTGGA
GGAACTGGGCTGTCCGAAATTCTTCCAGGATGTCACCCGCCAGCCACGCGGCCTGATCGTGGTCACCGGACCGACGGGTT
CGGGCAAGTCCACCACGCTGGCGGCCATGATCGATTACATCAACTCCAACGACTATTCTCACATTCTGACCATCGAGGAT
CCGATCGAGTTCGTCCACCAAAGCAAGAAATGCCTGATCAACCAGCGCGAGGTGCACCGTAACACCTTGGGCTTCAACGA
AGCCCTGCGCTCCGCATTGCGGGAGGACCCGGACGTGATCCTGGTGGGGGAAATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGTTCCGCCGCCAAGACCATCGACCGCATC
GTCGACGTATTTCCCGCCGCGGAGAAAGAGATGATCCGCTCCATGCTGTCGGAATCGCTGCAAGCGGTTATTTCGCAGGC
TCTGGTCAAGAAGGCCGGCGGCGGCCGGACCGCGGCCTGGGAGATCATGGTCGGCACACCGGCCATCCGCAACCTGATCC
GCGAGGCCAAGGTGGCCCAGATGTATTCGACGATCCAGACCGGCCGCAAGGACGGCATGCAGACCCTGGACCAGCACCTG
CAGGAGCTGGTGGAGAAAGGGATCGTCACCCGGCAGGTCGCCCGCGAAGTCGCCGTCAACAAAGCCGCGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q608F5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.065

98.256

0.797
  pilT Pseudomonas stutzeri DSM 10701

78.779

100

0.788
  pilT Acinetobacter nosocomialis M2

77.616

100

0.776
  pilT Acinetobacter baumannii D1279779

77.616

100

0.776
  pilT Acinetobacter baumannii strain A118

77.616

100

0.776
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Legionella pneumophila strain Lp02

74.709

100

0.747
  pilT Legionella pneumophila strain ERS1305867

74.709

100

0.747
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.667

100

0.669
  pilT Vibrio cholerae strain A1552

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Vibrio cholerae strain A1552

40

97.384

0.39
  pilU Acinetobacter baylyi ADP1

39.755

95.058

0.378