Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M2J80_RS24055 Genome accession   NZ_CP097103
Coordinates   5307448..5308446 (-) Length   332 a.a.
NCBI ID   WP_161892696.1    Uniprot ID   A0AAX3KGC6
Organism   Pseudomonas sp. NY11382     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5302448..5313446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2J80_RS24035 (M2J80_24020) argS 5303399..5305135 (+) 1737 WP_009684939.1 arginine--tRNA ligase -
  M2J80_RS24040 (M2J80_24025) - 5305137..5305835 (+) 699 WP_047149606.1 SPOR domain-containing protein -
  M2J80_RS24045 (M2J80_24030) - 5305932..5306351 (-) 420 WP_009684937.1 NINE protein -
  M2J80_RS24050 (M2J80_24035) - 5306518..5307141 (+) 624 WP_161892695.1 C40 family peptidase -
  M2J80_RS24055 (M2J80_24040) pilT 5307448..5308446 (-) 999 WP_161892696.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M2J80_RS24060 (M2J80_24045) - 5308512..5309207 (+) 696 WP_161892697.1 YggS family pyridoxal phosphate-dependent enzyme -
  M2J80_RS24065 (M2J80_24050) proC 5309246..5310064 (+) 819 WP_110677653.1 pyrroline-5-carboxylate reductase -
  M2J80_RS24070 (M2J80_24055) - 5310075..5310665 (+) 591 WP_009684930.1 YggT family protein -
  M2J80_RS24075 (M2J80_24060) - 5310754..5311893 (+) 1140 WP_009684929.1 homoserine O-acetyltransferase -
  M2J80_RS24080 (M2J80_24065) metW 5311901..5312521 (+) 621 WP_054912845.1 methionine biosynthesis protein MetW -
  M2J80_RS24085 (M2J80_24070) rdgB 5312648..5313244 (+) 597 WP_033690803.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35617.22 Da        Isoelectric Point: 6.7611

>NTDB_id=687065 M2J80_RS24055 WP_161892696.1 5307448..5308446(-) (pilT) [Pseudomonas sp. NY11382]
MDVTDLLARAVAVQASDLHLAAGQTPMLRLDGELQRMAMPPLAAGALVEGMATLLDDTQREQWAQGAELDLALALPALGR
FRVNLFRQLNGPAATFRLIPGRIATLDELALTDVFQAVVQYRDGLVLVGGPTGSGKSSTLATLLDQLNREKALHIITLED
PVEVIHSSQRSLVNQREVGRHSSGFAQGLRSALRQDPDVIMIGELRDLQTVRLALQAAETGHLVLATVHARSAASSIERL
VEVFAADEKPLVRAMLAESLQMVVAQVLVRRVGGGRVAAREVLVATPAVRNLVREGRMAQLCSVMQAGGAQGMRTMEGAL
RGLREKGLVDDC

Nucleotide


Download         Length: 999 bp        

>NTDB_id=687065 M2J80_RS24055 WP_161892696.1 5307448..5308446(-) (pilT) [Pseudomonas sp. NY11382]
ATGGATGTGACCGACCTGTTGGCACGGGCCGTGGCGGTGCAGGCTTCGGACCTGCACCTGGCGGCCGGCCAGACACCCAT
GCTGCGCCTGGACGGCGAGCTGCAGCGCATGGCCATGCCGCCGCTCGCCGCGGGCGCCTTGGTCGAGGGCATGGCAACCC
TGCTCGATGACACGCAGCGCGAGCAGTGGGCGCAAGGGGCTGAACTGGACCTGGCGCTGGCGCTGCCTGCCCTAGGGCGC
TTTCGCGTGAACCTGTTTCGCCAGCTGAATGGGCCTGCGGCGACCTTCCGCCTGATCCCAGGGCGTATTGCCACGCTGGA
TGAGCTAGCGCTGACCGATGTGTTTCAGGCGGTGGTCCAGTACCGCGATGGTCTGGTGCTGGTGGGTGGGCCGACCGGCA
GTGGCAAGTCCAGCACACTGGCAACGTTGCTGGACCAGCTGAACCGCGAAAAGGCGCTGCACATCATTACCCTGGAAGAC
CCTGTTGAAGTTATCCACAGCAGCCAGCGTAGCCTGGTCAACCAGCGCGAGGTCGGCCGCCACAGCAGCGGCTTTGCCCA
GGGGTTACGCAGCGCGCTGCGCCAGGACCCGGACGTGATCATGATCGGCGAGTTGCGCGACCTGCAGACCGTTCGCCTGG
CCTTGCAAGCGGCCGAGACCGGGCACCTGGTGCTGGCGACGGTACATGCGCGCTCGGCGGCCAGCAGCATCGAGCGGCTG
GTGGAGGTATTTGCAGCCGATGAAAAGCCGTTGGTGCGGGCGATGCTGGCGGAGTCGTTGCAAATGGTGGTGGCGCAGGT
GCTGGTACGCCGCGTGGGCGGTGGGCGGGTTGCAGCGCGGGAAGTGTTGGTGGCGACGCCGGCGGTGCGCAACCTGGTGC
GCGAGGGGCGGATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGTGGTGCGCAAGGGATGCGGACGATGGAAGGGGCATTG
CGTGGGTTGCGAGAGAAAGGCCTGGTTGACGATTGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.976

99.096

0.515
  pilT Pseudomonas stutzeri DSM 10701

50.76

99.096

0.503
  pilT Acinetobacter baylyi ADP1

50

99.398

0.497
  pilT Vibrio cholerae strain A1552

49.394

99.398

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.394

99.398

0.491
  pilT Acinetobacter baumannii strain A118

49.544

99.096

0.491
  pilT Acinetobacter nosocomialis M2

49.544

99.096

0.491
  pilT Acinetobacter baumannii D1279779

49.544

99.096

0.491
  pilT Neisseria meningitidis 8013

48.182

99.398

0.479
  pilT Neisseria gonorrhoeae MS11

47.879

99.398

0.476
  pilT Legionella pneumophila strain ERS1305867

48.024

99.096

0.476
  pilT Legionella pneumophila strain Lp02

48.024

99.096

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.545

99.398

0.443
  pilU Vibrio cholerae strain A1552

36.747

100

0.367
  pilU Pseudomonas stutzeri DSM 10701

37.195

98.795

0.367