Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M2B19_RS20315 Genome accession   NZ_CP096849
Coordinates   4179912..4180892 (-) Length   326 a.a.
NCBI ID   WP_045281449.1    Uniprot ID   -
Organism   Enterobacter kobei strain EC1382     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4174912..4185892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2B19_RS20285 (M2B19_20285) - 4175801..4176298 (+) 498 WP_014885186.1 SprT family zinc-dependent metalloprotease -
  M2B19_RS20290 (M2B19_20290) endA 4176393..4177100 (+) 708 WP_045134810.1 deoxyribonuclease I -
  M2B19_RS20295 (M2B19_20295) rsmE 4177152..4177883 (+) 732 WP_023337758.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M2B19_RS20300 (M2B19_20300) gshB 4177903..4178850 (+) 948 WP_023331278.1 glutathione synthase -
  M2B19_RS20305 (M2B19_20305) - 4178925..4179485 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  M2B19_RS20310 (M2B19_20310) ruvX 4179485..4179901 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  M2B19_RS20315 (M2B19_20315) pilT 4179912..4180892 (-) 981 WP_045281449.1 type IV pilus twitching motility protein PilT Machinery gene
  M2B19_RS20320 (M2B19_20320) - 4180910..4181611 (+) 702 WP_014885191.1 YggS family pyridoxal phosphate-dependent enzyme -
  M2B19_RS20325 (M2B19_20325) - 4181633..4182199 (+) 567 WP_014885192.1 YggT family protein -
  M2B19_RS20330 (M2B19_20330) yggU 4182196..4182492 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  M2B19_RS20335 (M2B19_20335) - 4182496..4183089 (+) 594 WP_309164634.1 XTP/dITP diphosphatase -
  M2B19_RS20340 (M2B19_20340) hemW 4183082..4184224 (+) 1143 WP_045281448.1 radical SAM family heme chaperone HemW -
  M2B19_RS20345 (M2B19_20345) - 4184288..4184626 (+) 339 WP_023331282.1 endonuclease domain-containing protein -
  M2B19_RS20350 (M2B19_20350) - 4184708..4185424 (-) 717 WP_014885197.1 DUF2884 domain-containing protein -
  M2B19_RS20355 (M2B19_20355) - 4185482..4185808 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35620.59 Da        Isoelectric Point: 6.4113

>NTDB_id=684703 M2B19_RS20315 WP_045281449.1 4179912..4180892(-) (pilT) [Enterobacter kobei strain EC1382]
MDVEEMVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVGTLLKAWLNDEQQGSWWAHGQVDFAVTVAEGQRLRG
SAFKQINGVSIALRLLPRTCSPLSSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
YLYRSERCLIQQREIGQHCPSFVEALRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSVAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=684703 M2B19_RS20315 WP_045281449.1 4179912..4180892(-) (pilT) [Enterobacter kobei strain EC1382]
ATGGATGTGGAAGAAATGGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCCGGTCAGGACGCCTTGAACCCGCGCCGTTTCCGCCGCCTGATGTCGGGACGTTATTAAAAGCGTGGCTCAATG
ATGAGCAGCAGGGAAGCTGGTGGGCTCACGGTCAGGTCGATTTTGCCGTGACGGTGGCAGAAGGCCAGCGTCTGCGCGGG
AGTGCGTTTAAGCAGATTAACGGGGTGTCCATCGCCCTGCGGTTGCTGCCACGCACCTGTTCGCCACTCTCTTCGCTGGG
CGTGCCGCGTGCCATTCCGGAACTGTTATCCAGTGACAACGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGTAAAT
CCACCACTCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGGCATATCCTCACGCTTGAAGATCCGGTGGAG
TATCTGTACCGGAGCGAACGCTGCCTGATTCAGCAGCGGGAAATAGGTCAGCACTGCCCGTCTTTTGTCGAGGCGCTGCG
CGGAGCGTTACGCGAGGATCCAGATGTGATTTTGCTGGGGGAGCTGCGTGATGCAGAGACGATCCGTCTCGCGTTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACACTGCATACGCGGGGTGCCTCGCAGGCCATTGAGCGGCTGGTGGACACC
TTCCCGGCGCAGGAGAAAGACCCGGTACGTAACCAGCTGGCCGGTAGCCTGCGTGCGGTGCTGGCACAGAGGCTGCTTCC
CGACCTGCAGGGTGGGCGTGTCGCGCTGTATGAACTGCTGGTGAATACCCCGGCGGCGGCAAATCTGATTCGTGAAGGAA
AAACGTGGCAACTGCCCGGTATCATTCAAACAGGCCAGCAGGCGGGAATGCAGAACTTTGATCAGAGCGTGGCGGAGAGA
CGGGCGCAGGGGCGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.041

100

0.482
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38