Detailed information NTG0681834

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: M1B72_RS20935
• Genome accession: NZ_CP096574
• Coordinates: 4918384..4919457 (-)
• Length: 357 a.a.
• NCBI ID: WP_183344655.1
• Uniprot ID: A0A6V8MR34
• Organism: Geomonas paludis strain RG22
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4913384..4924457

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M1B72_RS20920 (M1B72_20920)	-	4915256..4915474 (-)	219	WP_183344661.1	hypothetical protein	-
M1B72_RS20925 (M1B72_20925)	-	4915945..4916784 (+)	840	WP_183344659.1	alpha/beta fold hydrolase	-
M1B72_RS20930 (M1B72_20930)	-	4916993..4918162 (-)	1170	WP_183344657.1	aminotransferase	-
M1B72_RS20935 (M1B72_20935)	pilT	4918384..4919457 (-)	1074	WP_183344655.1	type IV pilus twitching motility protein PilT	Machinery gene
M1B72_RS20940 (M1B72_20940)	-	4919482..4921140 (-)	1659	WP_183344653.1	GspE/PulE family protein	-
M1B72_RS20945 (M1B72_20945)	-	4921488..4922492 (+)	1005	WP_183344645.1	hypothetical protein	-

Sequence

Protein

• Length: 357 a.a.
• Molecular weight: 39978.96 Da
• Isoelectric Point: 7.0675

>NTDB_id=681834 M1B72_RS20935 WP_183344655.1 4918384..4919457(-) (pilT) [Geomonas paludis strain RG22]
MARIDALFKLLNDAGASDLHLSSGSPPIFRLRGEMERQNFKSLTHEELKAILYEILTPKQREQFEETHDLDFAYSVPGLA
RFRGNYMMQHRGIAAVFRIIPSKILSADDLGLPDGIRNMTKLRKGMVLVTGPTGSGKSTTLAAMIDLINSTRREHILTLE
DPLEFIHENKMSLFNQRQIGEHSQSFASALRAALREDPDVILVGEMRDLETISLAMSAAETGHLVFGTLHTSSAAKTVDR
IIDVFPKDAQEQVRAILSESLKGVVCQQLLKTADGKGRAAAQEIMVWNPAIGNLIREGKTFQIPSIMQTGRKDGMQLMDQ
HILDLLKTKRVSPEEAYRCCQDKRQFEQYLTAPPQEH

Nucleotide

• Length: 1074 bp

>NTDB_id=681834 M1B72_RS20935 WP_183344655.1 4918384..4919457(-) (pilT) [Geomonas paludis strain RG22]
ATGGCAAGGATTGATGCGCTATTCAAGCTGCTAAACGACGCGGGAGCTTCCGATTTGCACCTCTCTTCGGGGTCCCCGCC
GATCTTCCGTTTGCGCGGTGAGATGGAACGCCAGAACTTCAAGTCTCTGACCCATGAAGAGCTGAAGGCGATCCTGTACG
AGATACTGACCCCCAAGCAGCGCGAGCAGTTCGAGGAGACGCACGACCTCGACTTCGCCTACTCGGTGCCGGGGCTGGCG
CGCTTCCGCGGCAACTACATGATGCAGCATCGCGGCATCGCGGCCGTGTTCCGCATCATCCCGAGCAAGATCCTGTCCGC
CGACGACCTGGGGCTCCCCGACGGCATCCGCAACATGACCAAGCTCAGGAAGGGGATGGTGCTGGTCACCGGCCCCACCG
GGAGCGGCAAGTCGACCACCCTGGCCGCCATGATCGACCTGATCAACAGCACCCGCCGCGAGCACATCCTGACCCTCGAG
GACCCGCTGGAGTTCATCCACGAGAACAAGATGTCGCTGTTCAACCAGCGCCAGATCGGCGAGCACTCCCAGAGCTTCGC
CAGCGCGCTCAGGGCGGCACTCAGGGAGGACCCGGACGTCATCCTGGTCGGCGAGATGCGCGACCTGGAGACCATCAGCC
TCGCCATGAGCGCCGCCGAGACCGGTCACCTCGTCTTCGGCACCCTGCACACCAGTTCCGCCGCGAAGACGGTGGACCGT
ATCATCGACGTGTTCCCGAAGGACGCCCAGGAGCAGGTACGCGCCATCCTCTCCGAGTCGCTCAAGGGGGTCGTCTGCCA
GCAGCTTCTGAAGACCGCGGACGGCAAGGGGCGCGCCGCCGCGCAGGAGATCATGGTCTGGAACCCGGCCATCGGCAACC
TGATCCGCGAGGGCAAGACCTTCCAGATCCCCTCCATCATGCAGACCGGCAGGAAGGACGGCATGCAGCTCATGGATCAG
CACATCCTGGACCTGTTGAAGACCAAGCGCGTGTCACCCGAGGAAGCCTACCGCTGCTGCCAGGACAAGCGCCAGTTCGA
GCAGTACCTGACCGCGCCGCCGCAGGAGCATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (11-269)

3D structure

• AlphaFold DB: A0A6V8MR34

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Pseudomonas stutzeri DSM 10701	55.422	92.997	0.515
pilT	Legionella pneumophila strain Lp02	55.589	92.717	0.515
pilT	Legionella pneumophila strain ERS1305867	55.589	92.717	0.515
pilT	Neisseria meningitidis 8013	55.963	91.597	0.513
pilT	Acinetobacter baumannii strain A118	55.12	92.997	0.513
pilT	Acinetobacter nosocomialis M2	55.12	92.997	0.513
pilT	Acinetobacter baumannii D1279779	55.12	92.997	0.513
pilT	Neisseria gonorrhoeae MS11	55.657	91.597	0.51
pilT	Acinetobacter baylyi ADP1	58.199	87.115	0.507
pilT	Pseudomonas aeruginosa PAK	55.59	90.196	0.501
pilT	Vibrio cholerae strain A1552	53.583	89.916	0.482
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	53.583	89.916	0.482
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	49.271	96.078	0.473
pilU	Acinetobacter baylyi ADP1	38.739	93.277	0.361
pilU	Pseudomonas stutzeri DSM 10701	38.855	92.997	0.361