Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MXB02_RS24920 Genome accession   NZ_CP095556
Coordinates   5381653..5382657 (-) Length   334 a.a.
NCBI ID   WP_028689121.1    Uniprot ID   -
Organism   Pseudomonas mosselii strain 923     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5376653..5387657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MXB02_RS24900 (MXB02_24900) argS 5377897..5379633 (+) 1737 WP_028689117.1 arginine--tRNA ligase -
  MXB02_RS24905 (MXB02_24905) - 5379635..5380339 (+) 705 WP_062362824.1 SPOR domain-containing protein -
  MXB02_RS24910 (MXB02_24910) - 5380380..5380805 (-) 426 WP_028689119.1 TM2 domain-containing protein -
  MXB02_RS24915 (MXB02_24915) - 5380963..5381613 (+) 651 WP_069016536.1 NlpC/P60 family protein -
  MXB02_RS24920 (MXB02_24920) pilT 5381653..5382657 (-) 1005 WP_028689121.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MXB02_RS24925 (MXB02_24925) - 5382723..5383409 (+) 687 WP_062575475.1 YggS family pyridoxal phosphate-dependent enzyme -
  MXB02_RS24930 (MXB02_24930) proC 5383448..5384266 (+) 819 WP_207851480.1 pyrroline-5-carboxylate reductase -
  MXB02_RS24935 (MXB02_24935) - 5384277..5384867 (+) 591 WP_028689124.1 YggT family protein -
  MXB02_RS24940 (MXB02_24940) - 5384956..5386095 (+) 1140 WP_028689125.1 homoserine O-acetyltransferase -
  MXB02_RS24945 (MXB02_24945) metW 5386103..5386723 (+) 621 WP_011531735.1 methionine biosynthesis protein MetW -
  MXB02_RS24950 (MXB02_24950) - 5386749..5387183 (+) 435 WP_028689126.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36236.79 Da        Isoelectric Point: 6.7020

>NTDB_id=677488 MXB02_RS24920 WP_028689121.1 5381653..5382657(-) (pilT) [Pseudomonas mosselii strain 923]
MDVTDLLRRALAAAASDLHLAAGERPLLRVDGVLQRLDLPVLTAGTLEDGMAAVLDDEQRRQWAAGEELDLAVSLDGLGR
FRLNLFRQRNGPSASVRLIPASVPCLEVLELVDVFQIISQYRDGFILVGGATGSGKSSTLAALIDRLNREYALHIVTLED
PIEFIHASQRSLVNQREVGLHSRDFAQALRSALRQDPDVIMIGELRDLHSIRLAMRAAETGHLVLATVHTRSAVSSIDRL
VEVFAPEEKPLVRSMLADSLRLVVAQVLVRRSGGGRVAAREVLVATPAVRNLIREGRLAQIHSLMQAGAAVGMCTMEGAL
QALEARGIIDRASN

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=677488 MXB02_RS24920 WP_028689121.1 5381653..5382657(-) (pilT) [Pseudomonas mosselii strain 923]
ATGGATGTGACCGACCTGTTGCGCAGGGCCCTGGCTGCCGCTGCCAGCGACCTGCACCTGGCCGCGGGCGAGCGGCCGCT
GCTGCGCGTGGATGGGGTGTTGCAGCGCCTGGACCTGCCGGTGCTGACGGCAGGAACGCTGGAAGACGGGATGGCTGCCG
TGCTTGATGACGAGCAGCGGCGACAGTGGGCTGCCGGCGAAGAGTTGGACCTGGCGGTGAGCCTGGATGGGCTGGGGCGC
TTTCGGCTGAACCTGTTCCGCCAGCGCAATGGCCCGAGTGCCAGCGTGCGTTTGATCCCCGCGAGCGTGCCCTGTCTGGA
GGTGCTTGAGCTGGTAGATGTATTTCAAATCATCTCCCAGTACCGGGATGGCTTCATCTTGGTGGGCGGGGCCACCGGCA
GTGGCAAGTCGAGCACCCTGGCGGCGCTGATCGATCGGCTCAACCGCGAATACGCATTGCATATCGTCACGCTCGAGGAC
CCGATCGAGTTCATCCACGCCAGCCAGCGCAGCCTGGTCAACCAGCGTGAGGTCGGCCTGCATAGCCGTGACTTTGCCCA
GGCCCTGCGCAGCGCCCTGCGTCAGGACCCGGACGTGATCATGATCGGTGAGCTGCGCGATCTGCACAGCATTCGCCTGG
CGATGCGCGCCGCGGAGACCGGGCACCTGGTACTGGCCACCGTGCATACGCGATCGGCGGTAAGCAGTATCGACCGGCTG
GTGGAGGTGTTCGCGCCGGAGGAAAAGCCGCTGGTGCGCAGCATGCTGGCCGACTCCCTGCGGCTGGTGGTCGCGCAGGT
GCTGGTTCGGCGATCAGGCGGCGGTCGGGTCGCGGCGCGGGAGGTGCTGGTGGCGACGCCGGCGGTGCGCAACCTGATCC
GCGAGGGGCGTCTGGCGCAGATTCATTCGCTGATGCAGGCTGGTGCGGCTGTGGGGATGTGCACGATGGAGGGTGCGCTG
CAGGCGCTGGAAGCGCGGGGGATTATCGACAGGGCCTCAAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

49.249

99.701

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.394

98.802

0.488
  pilT Vibrio cholerae strain A1552

49.394

98.802

0.488
  pilT Pseudomonas stutzeri DSM 10701

48.949

99.701

0.488
  pilT Acinetobacter baylyi ADP1

49.091

98.802

0.485
  pilT Neisseria meningitidis 8013

48.788

98.802

0.482
  pilT Acinetobacter baumannii D1279779

48.936

98.503

0.482
  pilT Acinetobacter baumannii strain A118

48.936

98.503

0.482
  pilT Neisseria gonorrhoeae MS11

48.485

98.802

0.479
  pilT Acinetobacter nosocomialis M2

48.632

98.503

0.479
  pilT Legionella pneumophila strain Lp02

47.734

99.102

0.473
  pilT Legionella pneumophila strain ERS1305867

47.734

99.102

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.758

98.802

0.452
  pilU Pseudomonas stutzeri DSM 10701

38.485

98.802

0.38
  pilU Vibrio cholerae strain A1552

38.889

97.006

0.377