Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MW400_RS20660 Genome accession   NZ_CP095533
Coordinates   4410000..4410980 (-) Length   326 a.a.
NCBI ID   WP_072265128.1    Uniprot ID   -
Organism   Escherichia coli strain GN02461     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4405000..4415980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW400_RS20630 (MW400_20620) yggI 4405724..4406221 (+) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -
  MW400_RS20635 (MW400_20625) endA 4406316..4407023 (+) 708 WP_000286497.1 deoxyribonuclease I -
  MW400_RS20640 (MW400_20630) rsmE 4407103..4407834 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MW400_RS20645 (MW400_20635) gshB 4407847..4408797 (+) 951 WP_000593273.1 glutathione synthase -
  MW400_RS20650 (MW400_20640) yqgE 4408906..4409469 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  MW400_RS20655 (MW400_20645) ruvX 4409469..4409885 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  MW400_RS20660 (MW400_20650) pilT 4410000..4410980 (-) 981 WP_072265128.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MW400_RS20665 (MW400_20655) yggS 4410998..4411702 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  MW400_RS20670 (MW400_20660) yggT 4411720..4412286 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  MW400_RS20675 (MW400_20665) yggU 4412283..4412573 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  MW400_RS20680 (MW400_20670) rdgB 4412581..4413174 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  MW400_RS20685 (MW400_20675) hemW 4413167..4414303 (+) 1137 WP_000239941.1 radical SAM family heme chaperone HemW -
  MW400_RS20690 (MW400_20680) - 4414618..4415604 (+) 987 WP_000784011.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36006.25 Da        Isoelectric Point: 6.1759

>NTDB_id=677410 MW400_RS20660 WP_072265128.1 4410000..4410980(-) (pilT) [Escherichia coli strain GN02461]
MYMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFKLLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=677410 MW400_RS20660 WP_072265128.1 4410000..4410980(-) (pilT) [Escherichia coli strain GN02461]
ATGTATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGTTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCTAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CAGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCCCAGGCAGTTGAGCGGCTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTAAATTGCTGGTTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGAATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.089

96.933

0.408
  pilU Vibrio cholerae strain A1552

40.063

97.239

0.39
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374