Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MUB29_RS18630 Genome accession   NZ_CP094943
Coordinates   4230481..4231494 (-) Length   337 a.a.
NCBI ID   WP_263063616.1    Uniprot ID   -
Organism   Dickeya dadantii strain FZ06     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4225481..4236494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUB29_RS18610 - 4227243..4228154 (+) 912 WP_226053825.1 CdaR family transcriptional regulator -
  MUB29_RS18615 codA 4228157..4229407 (+) 1251 WP_263063613.1 cytosine deaminase -
  MUB29_RS18620 - 4229545..4230165 (+) 621 WP_263063615.1 Fic family protein -
  MUB29_RS18625 - 4230144..4230335 (-) 192 WP_038923773.1 hypothetical protein -
  MUB29_RS18630 pilT 4230481..4231494 (-) 1014 WP_263063616.1 type IV pilus twitching motility protein PilT Machinery gene
  MUB29_RS18635 - 4231516..4232250 (+) 735 WP_263063617.1 YggS family pyridoxal phosphate-dependent enzyme -
  MUB29_RS18640 proC 4232284..4233105 (+) 822 WP_263063618.1 pyrroline-5-carboxylate reductase -
  MUB29_RS18645 - 4233149..4233703 (+) 555 WP_013319314.1 YggT family protein -
  MUB29_RS18650 yggU 4233700..4233990 (+) 291 WP_033112045.1 DUF167 family protein YggU -
  MUB29_RS18655 - 4234037..4234630 (+) 594 WP_013319316.1 XTP/dITP diphosphatase -
  MUB29_RS18660 hemW 4234623..4235762 (+) 1140 WP_263063620.1 radical SAM family heme chaperone HemW -
  MUB29_RS18665 - 4235887..4236015 (+) 129 WP_013319318.1 entericidin A/B family lipoprotein -

Sequence

Protein


Download         Length: 337 a.a.        Molecular weight: 36877.09 Da        Isoelectric Point: 6.7717

>NTDB_id=674367 MUB29_RS18630 WP_263063616.1 4230481..4231494(-) (pilT) [Dickeya dadantii strain FZ06]
MDVSEWVRSSVKQNVSDLHLCSGHPPIWRIDGQLVADAAQPPLHNAELEQWCRSWLEPACEQQLRQSGQTDCALTLPEGQ
RLRANLFMQRHGLSAALRLIPSHQPDLRTLGAPPVVAELVERADGLILVTGATGSGKSTTLAAMIAALNQHRARHIITLE
DPIEFLHTSQRSLIQQREIGRHSEGFASALRAALREDPDVLLLGELRDSETIRLALTAAETGHLVLATLHTRRAVQAVDR
LVDVFPAEEKAFVRAQLAASLQAVVAQKLLPGAQGGRVALFEVLLATPAVSNLIREGKTHQLPSLLQTGSQVGMQTFEQS
YQQRCREGALRPEINAD

Nucleotide


Download         Length: 1014 bp        

>NTDB_id=674367 MUB29_RS18630 WP_263063616.1 4230481..4231494(-) (pilT) [Dickeya dadantii strain FZ06]
ATGGATGTGAGTGAATGGGTGCGGTCTAGTGTAAAACAGAATGTGTCCGATCTGCACCTGTGCAGCGGTCATCCGCCGAT
TTGGCGTATCGACGGGCAACTGGTGGCGGATGCCGCGCAGCCGCCGTTGCACAACGCCGAACTGGAACAGTGGTGCCGTA
GCTGGCTGGAGCCGGCCTGCGAGCAGCAGCTACGGCAAAGCGGGCAGACCGATTGCGCGCTGACGCTGCCGGAGGGCCAG
CGGTTACGGGCTAATCTGTTTATGCAGCGCCACGGCCTGTCGGCGGCGTTACGCCTGATCCCGTCACACCAACCCGACCT
GCGAACGCTGGGCGCGCCGCCGGTGGTGGCGGAGCTGGTCGAGCGCGCCGATGGCCTGATTCTGGTGACCGGCGCCACCG
GCAGCGGTAAATCCACCACGCTGGCGGCGATGATTGCCGCGCTTAATCAACACCGCGCGCGCCATATCATTACGCTGGAA
GACCCGATTGAGTTCCTGCATACCAGCCAGCGCAGCCTGATCCAGCAGCGGGAGATCGGCCGTCACAGCGAAGGATTCGC
CAGCGCATTACGGGCGGCGCTGCGCGAAGACCCGGACGTACTGTTGCTCGGCGAGCTGCGCGACAGCGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTACTGGCGACGCTGCATACCCGGCGAGCGGTGCAGGCGGTGGATCGG
CTGGTGGACGTTTTTCCCGCCGAAGAGAAAGCGTTTGTCCGCGCGCAACTGGCCGCCAGCCTGCAGGCCGTGGTGGCGCA
GAAACTGTTGCCGGGCGCGCAAGGCGGGCGCGTGGCGCTGTTTGAAGTGCTGCTCGCCACCCCGGCGGTCAGCAACCTGA
TCCGCGAAGGTAAAACCCACCAGTTGCCCAGCCTGCTGCAAACCGGCAGCCAGGTCGGCATGCAGACTTTTGAGCAGAGT
TACCAGCAGCGATGCCGGGAGGGTGCGTTGCGACCTGAGATCAATGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.697

97.923

0.487
  pilT Vibrio cholerae strain A1552

49.697

97.923

0.487
  pilT Neisseria meningitidis 8013

48.955

99.407

0.487
  pilT Neisseria gonorrhoeae MS11

48.802

99.11

0.484
  pilT Pseudomonas stutzeri DSM 10701

48.485

97.923

0.475
  pilT Pseudomonas aeruginosa PAK

47.748

98.813

0.472
  pilT Acinetobacter baylyi ADP1

48.182

97.923

0.472
  pilT Acinetobacter baumannii D1279779

47.447

98.813

0.469
  pilT Acinetobacter nosocomialis M2

47.447

98.813

0.469
  pilT Acinetobacter baumannii strain A118

47.447

98.813

0.469
  pilT Legionella pneumophila strain ERS1305867

48.903

94.659

0.463
  pilT Legionella pneumophila strain Lp02

48.903

94.659

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.141

94.659

0.427
  pilU Vibrio cholerae strain A1552

39.198

96.142

0.377
  pilU Pseudomonas stutzeri DSM 10701

37.126

99.11

0.368