Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AST72_RS15410 Genome accession   NZ_AP014856
Coordinates   3023660..3024640 (-) Length   326 a.a.
NCBI ID   WP_095576007.1    Uniprot ID   -
Organism   Escherichia albertii strain CB9786     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3018660..3029640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AST72_RS15380 (EACBF_2838) - 3019315..3019812 (+) 498 WP_002461299.1 SprT family zinc-dependent metalloprotease -
  AST72_RS15385 (EACBF_2839) endA 3019907..3020614 (+) 708 WP_002461298.1 deoxyribonuclease I -
  AST72_RS15390 (EACBF_2840) rsmE 3020694..3021425 (+) 732 WP_002461297.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AST72_RS15395 (EACBF_2841) gshB 3021438..3022388 (+) 951 WP_000593300.1 glutathione synthase -
  AST72_RS15400 (EACBF_2842) - 3022497..3023060 (+) 564 WP_002461296.1 YqgE/AlgH family protein -
  AST72_RS15405 (EACBF_2843) ruvX 3023060..3023494 (+) 435 WP_059220283.1 Holliday junction resolvase RuvX -
  AST72_RS15410 (EACBF_2844) pilT 3023660..3024640 (-) 981 WP_095576007.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AST72_RS15415 (EACBF_2845) yggS 3024658..3025362 (+) 705 WP_059220282.1 pyridoxal phosphate homeostasis protein -
  AST72_RS15420 (EACBF_2846) yggT 3025380..3025946 (+) 567 WP_001094839.1 osmotic shock tolerance protein YggT -
  AST72_RS15425 (EACBF_2847) yggU 3025943..3026233 (+) 291 WP_059220281.1 DUF167 family protein YggU -
  AST72_RS15430 (EACBF_2848) - 3026241..3026834 (+) 594 WP_059275583.1 XTP/dITP diphosphatase -
  AST72_RS15435 (EACBF_2849) hemW 3026827..3027963 (+) 1137 WP_059275584.1 radical SAM family heme chaperone HemW -
  AST72_RS15440 (EACBF_2850) - 3028028..3029035 (-) 1008 WP_059275585.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36075.23 Da        Isoelectric Point: 5.5860

>NTDB_id=67100 AST72_RS15410 WP_095576007.1 3023660..3024640(-) (pilT) [Escherichia albertii strain CB9786]
MNMEEIVALSVKHNVSDLHLCSVWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRVLLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=67100 AST72_RS15410 WP_095576007.1 3023660..3024640(-) (pilT) [Escherichia albertii strain CB9786]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGTCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACGTTGAAGAGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGTGTTATTGCTGGAGAATGGGCAGTTGGATTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGCCCGCAACTCGAACAGCTTGG
TGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTAATTTTGCTTGGCGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCGGCTCAGGCGGTCGAGCGGCTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

38.554

100

0.393

Multiple sequence alignment