Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   MOW14_RS15010 Genome accession   NZ_CP094258
Coordinates   2646947..2647381 (-) Length   144 a.a.
NCBI ID   WP_180006571.1    Uniprot ID   -
Organism   Acinetobacter indicus strain BDT201     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2630958..2646880 2646947..2647381 flank 67


Gene organization within MGE regions


Location: 2630958..2647381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MOW14_RS15005 - 2630958..2631593 (-) 636 WP_104473453.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MOW14_RS12635 (MOW14_12635) - 2631598..2635449 (-) 3852 WP_180006578.1 hypothetical protein -
  MOW14_RS12640 (MOW14_12640) - 2635459..2636214 (-) 756 WP_180006577.1 hypothetical protein -
  MOW14_RS12645 (MOW14_12645) - 2636225..2637223 (-) 999 WP_180006631.1 PilW family protein -
  MOW14_RS12650 (MOW14_12650) pilV 2637226..2637729 (-) 504 WP_180006576.1 type IV pilus modification protein PilV -
  MOW14_RS12655 (MOW14_12655) - 2637726..2638262 (-) 537 WP_180006575.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MOW14_RS12660 (MOW14_12660) ispH 2638426..2639376 (-) 951 WP_180006630.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  MOW14_RS12665 (MOW14_12665) gmk 2639512..2640135 (+) 624 WP_045795273.1 guanylate kinase -
  MOW14_RS12670 (MOW14_12670) rpoZ 2640212..2640493 (+) 282 WP_004809957.1 DNA-directed RNA polymerase subunit omega -
  MOW14_RS12675 (MOW14_12675) - 2640717..2642816 (+) 2100 WP_180006574.1 HD domain-containing protein -
  MOW14_RS12680 (MOW14_12680) - 2642888..2643271 (+) 384 WP_005180461.1 RidA family protein -
  MOW14_RS12685 (MOW14_12685) - 2643426..2643620 (+) 195 WP_034597991.1 bacterioferritin-associated ferredoxin -
  MOW14_RS12690 (MOW14_12690) bfr 2643847..2644311 (+) 465 WP_005180468.1 bacterioferritin -
  MOW14_RS12695 (MOW14_12695) - 2644423..2646063 (-) 1641 WP_180006573.1 O-antigen ligase family protein -
  MOW14_RS12700 (MOW14_12700) tfpZ 2646155..2646880 (-) 726 WP_180006572.1 TfpX/TfpZ family type IV pilin accessory protein -
  MOW14_RS15010 pilA/pilA1 2646947..2647381 (-) 435 WP_180006571.1 pilin Machinery gene

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14818.68 Da        Isoelectric Point: 4.0174

>NTDB_id=668445 MOW14_RS15010 WP_180006571.1 2646947..2647381(-) (pilA/pilA1) [Acinetobacter indicus strain BDT201]
MKSVQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQASEALSATAGVRADMGVYLSEEGALPAAGDSATIDEALDEL
GGKYFDEGTVTLSAAGTISVPFSSGVHQGNTMTIAAQANTAGNQISSWQCDGLDDKYLPSACRP

Nucleotide


Download         Length: 435 bp        

>NTDB_id=668445 MOW14_RS15010 WP_180006571.1 2646947..2647381(-) (pilA/pilA1) [Acinetobacter indicus strain BDT201]
ATGAAATCAGTTCAAAAGGGTTTTACTCTTATCGAATTAATGATCGTTGTTGCTATTATCGGTATTCTTGCAGCTATTGC
GATTCCTGCTTATCAAGATTATACAGCACGTGCTCAAGCGTCTGAAGCACTTTCTGCAACTGCTGGTGTTCGTGCAGATA
TGGGTGTTTATCTTTCTGAAGAAGGTGCGCTTCCTGCAGCAGGTGACAGTGCTACTATTGATGAGGCATTAGATGAGCTA
GGTGGTAAATACTTTGATGAAGGTACGGTTACATTATCGGCTGCTGGAACAATTTCTGTTCCGTTTTCAAGCGGTGTTCA
CCAAGGTAATACGATGACGATTGCTGCGCAAGCAAACACGGCTGGTAATCAAATTTCTAGCTGGCAGTGTGATGGTTTAG
ATGACAAATACTTGCCATCTGCTTGCCGTCCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

48.684

100

0.514
  pilE Neisseria gonorrhoeae MS11

38.415

100

0.438
  pilE Neisseria gonorrhoeae strain FA1090

37.576

100

0.431
  comP Acinetobacter baylyi ADP1

41.781

100

0.424
  pilA2 Legionella pneumophila str. Paris

41.667

100

0.417
  pilA2 Legionella pneumophila strain ERS1305867

41.667

100

0.417
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.667

100

0.403
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.333

100

0.389
  pilA Pseudomonas aeruginosa PAK

37.415

100

0.382
  pilA Acinetobacter nosocomialis M2

50

76.389

0.382
  pilA Glaesserella parasuis strain SC1401

36.054

100

0.368
  pilA Vibrio cholerae strain A1552

36.111

100

0.361
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.111

100

0.361
  pilA Vibrio cholerae C6706

36.111

100

0.361
  pilA Ralstonia pseudosolanacearum GMI1000

50.98

70.833

0.361

Multiple sequence alignment