Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MOV42_RS08090 Genome accession   NZ_CP093396
Coordinates   1697790..1698872 (+) Length   360 a.a.
NCBI ID   WP_324170685.1    Uniprot ID   -
Organism   MAG: Sulfurimonas sp. isolate MAG-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1692790..1703872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MOV42_RS08055 - 1693070..1693780 (-) 711 WP_324170678.1 sulfite exporter TauE/SafE family protein -
  MOV42_RS08060 - 1693788..1694294 (-) 507 WP_324170679.1 hypothetical protein -
  MOV42_RS08065 - 1694435..1694908 (-) 474 WP_324170680.1 HD domain-containing protein -
  MOV42_RS08070 - 1694963..1695796 (-) 834 WP_324170681.1 tyrosine-type recombinase/integrase -
  MOV42_RS08075 - 1695771..1696760 (-) 990 WP_324170682.1 hypothetical protein -
  MOV42_RS08080 - 1696765..1697361 (-) 597 WP_324170683.1 hypothetical protein -
  MOV42_RS08085 gatC 1697480..1697770 (+) 291 WP_324170684.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  MOV42_RS08090 pilT 1697790..1698872 (+) 1083 WP_324170685.1 type IV pilus twitching motility protein PilT Machinery gene
  MOV42_RS08095 - 1698975..1701194 (+) 2220 WP_324170686.1 TonB-dependent receptor -
  MOV42_RS08100 exbB 1701219..1701662 (+) 444 WP_324170687.1 TonB-system energizer ExbB -
  MOV42_RS08105 - 1701652..1702032 (+) 381 WP_324170688.1 biopolymer transporter ExbD -
  MOV42_RS08110 - 1702046..1702705 (+) 660 WP_324170689.1 sulfite exporter TauE/SafE family protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40061.10 Da        Isoelectric Point: 6.1183

>NTDB_id=664364 MOV42_RS08090 WP_324170685.1 1697790..1698872(+) (pilT) [MAG: Sulfurimonas sp. isolate MAG-2]
MSEKMKKAVDIKKLLKSVLAYGSSDLHLVVGSEPQIRIDKELRPLNLAVLSAKDIEGMAYALIEDKQKKIFEDINELDFS
FELQDIGRFRANYYRTIGGIACAFRMIPIDIPTLDEYGNPPIFKELIKKEKGLILVTGPTGSGKSTTLASMLHEINMTER
RHVITVEDPVEFVHKNIKSLFSQRDVGNDTASFASALKFALRQDPDIILIGEMRDAETIGAALTAAETGHLVFGTLHTNS
APGTINRIIDVFDGEEQAQIRAQLASSLVAVVSQTLIPRVGGGKVATQEILITNPAIQNQIREDKVHQLYSQMQLNQNET
HMSTQTQELIELLQKRTITKENAIKNSNRPEELIKMIEGL

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=664364 MOV42_RS08090 WP_324170685.1 1697790..1698872(+) (pilT) [MAG: Sulfurimonas sp. isolate MAG-2]
ATGAGTGAAAAAATGAAAAAAGCAGTTGACATAAAAAAACTCCTAAAGAGTGTTTTAGCCTACGGTTCGAGTGATTTACA
CCTTGTTGTTGGAAGTGAACCACAAATTAGAATAGATAAAGAACTACGCCCACTTAATCTTGCTGTATTAAGTGCAAAAG
ATATTGAAGGAATGGCTTACGCACTTATAGAAGATAAACAAAAGAAAATTTTTGAAGATATAAACGAACTTGATTTTTCA
TTTGAGTTACAAGATATAGGTCGTTTTCGTGCTAATTACTACCGTACTATTGGTGGGATTGCTTGTGCTTTTCGTATGAT
TCCTATTGATATACCAACGCTTGATGAATATGGAAATCCTCCAATTTTTAAAGAACTTATAAAAAAAGAAAAAGGTCTTA
TTCTGGTTACTGGTCCAACAGGAAGTGGTAAATCAACCACATTAGCATCTATGCTTCATGAGATAAATATGACAGAAAGA
AGACATGTTATTACAGTTGAAGACCCAGTTGAGTTTGTTCATAAAAATATAAAATCTCTTTTCTCACAAAGAGATGTCGG
GAATGATACTGCTTCATTTGCATCTGCACTTAAATTTGCCCTGAGACAAGATCCAGATATCATACTAATTGGGGAAATGC
GAGATGCTGAAACTATTGGAGCAGCATTAACAGCGGCAGAGACAGGTCACTTGGTGTTTGGAACACTCCATACTAACTCT
GCACCTGGAACTATTAACCGTATTATAGATGTTTTTGATGGAGAAGAGCAAGCTCAAATTAGAGCGCAATTAGCTTCTTC
ACTTGTAGCAGTTGTCTCTCAAACATTAATACCTCGTGTTGGTGGTGGTAAAGTTGCAACACAAGAGATTTTAATAACAA
ACCCTGCTATCCAAAATCAGATTAGAGAAGACAAGGTTCATCAACTATACTCTCAAATGCAACTAAATCAAAATGAGACT
CACATGTCTACGCAAACGCAAGAATTAATAGAGTTACTTCAAAAAAGAACAATTACAAAAGAAAATGCTATTAAAAATTC
AAACAGACCTGAAGAGCTTATTAAAATGATAGAAGGGTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.725

95.833

0.486
  pilT Acinetobacter baylyi ADP1

50.578

96.111

0.486
  pilT Acinetobacter baumannii D1279779

50.289

96.111

0.483
  pilT Acinetobacter nosocomialis M2

50.289

96.111

0.483
  pilT Acinetobacter baumannii strain A118

50.289

96.111

0.483
  pilT Legionella pneumophila strain Lp02

51.94

93.056

0.483
  pilT Legionella pneumophila strain ERS1305867

51.94

93.056

0.483
  pilT Pseudomonas stutzeri DSM 10701

49.565

95.833

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.025

98.056

0.461
  pilT Neisseria gonorrhoeae MS11

48.387

94.722

0.458
  pilT Neisseria meningitidis 8013

48.094

94.722

0.456
  pilT Vibrio cholerae strain A1552

52.614

85

0.447
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.614

85

0.447
  pilU Acinetobacter baylyi ADP1

37.464

96.389

0.361
  pilU Vibrio cholerae strain A1552

36.827

98.056

0.361