Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MNO13_RS03585 Genome accession   NZ_CP093332
Coordinates   773592..774626 (+) Length   344 a.a.
NCBI ID   WP_241939536.1    Uniprot ID   -
Organism   Buttiauxella ferragutiae strain H4-C11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 768592..779626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNO13_RS03555 (MNO13_03555) - 768718..769896 (+) 1179 WP_241939534.1 sugar efflux transporter -
  MNO13_RS03560 (MNO13_03560) hemW 770255..771391 (-) 1137 WP_121814905.1 radical SAM family heme chaperone HemW -
  MNO13_RS03565 (MNO13_03565) - 771384..771977 (-) 594 WP_064543521.1 XTP/dITP diphosphatase -
  MNO13_RS03570 (MNO13_03570) yggU 772006..772296 (-) 291 WP_241939535.1 DUF167 family protein YggU -
  MNO13_RS03575 (MNO13_03575) - 772293..772847 (-) 555 WP_064543525.1 YggT family protein -
  MNO13_RS03580 (MNO13_03580) - 772872..773576 (-) 705 WP_121815739.1 YggS family pyridoxal phosphate-dependent enzyme -
  MNO13_RS03585 (MNO13_03585) pilT 773592..774626 (+) 1035 WP_241939536.1 type IV pilus twitching motility protein PilT Machinery gene
  MNO13_RS03590 (MNO13_03590) - 774711..775175 (+) 465 WP_241940041.1 type II toxin-antitoxin system HicB family antitoxin -
  MNO13_RS03595 (MNO13_03595) ruvX 775172..775591 (-) 420 WP_064543531.1 Holliday junction resolvase RuvX -
  MNO13_RS03600 (MNO13_03600) - 775591..776154 (-) 564 WP_064543533.1 YqgE/AlgH family protein -
  MNO13_RS03605 (MNO13_03605) gshB 776272..777219 (-) 948 WP_121814909.1 glutathione synthase -
  MNO13_RS03610 (MNO13_03610) rsmE 777234..777965 (-) 732 WP_121814910.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MNO13_RS03615 (MNO13_03615) endA 778041..778748 (-) 708 WP_064543538.1 deoxyribonuclease I -
  MNO13_RS03620 (MNO13_03620) - 778841..779356 (-) 516 WP_121814911.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37172.43 Da        Isoelectric Point: 6.2293

>NTDB_id=663690 MNO13_RS03585 WP_241939536.1 773592..774626(+) (pilT) [Buttiauxella ferragutiae strain H4-C11]
MDIEEIVALSVKHNVSDLHLCAQLPARWRRQGRLELLPGGAPEPQALLDDWLSKAQKEQLEESGQVDFAVTLKSGVRLRA
NAFHQLQGNSLALRLLASDCPKLEALNVPPLIPELLQSPDGLILVTGATGSGKSTTLAAMVEHLNHQLDGHILTLEDPIE
LVHQSKRCLIQQREPGTHCRSFAAALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDV
FPAEEKNMVRSQLAGSLKAVLAQKLQLDAQGGRVALFELLVNTPAVASLIREGKAHQLPGQIQTGQQYGMQSFSHSQARL
RAEGRIGIAPSPGGEGWGEGYSDD

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=663690 MNO13_RS03585 WP_241939536.1 773592..774626(+) (pilT) [Buttiauxella ferragutiae strain H4-C11]
ATGGATATCGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCTGATCTACACCTGTGCGCGCAGCTTCCTGCTCG
TTGGCGGCGGCAAGGACGGCTTGAACTACTGCCTGGCGGCGCGCCTGAACCACAAGCATTGCTTGATGACTGGCTGTCAA
AAGCACAAAAGGAACAACTGGAAGAAAGCGGGCAAGTGGATTTCGCCGTGACGCTAAAGTCAGGGGTACGGTTGCGGGCG
AACGCTTTTCACCAGCTGCAAGGCAACTCACTTGCCTTGCGGTTATTGGCGTCGGACTGTCCCAAACTCGAGGCGCTAAA
CGTTCCACCGTTGATCCCCGAATTGTTGCAATCCCCAGACGGGCTAATTCTGGTGACTGGCGCGACAGGCAGCGGTAAAT
CCACCACGCTTGCGGCGATGGTTGAGCATCTCAACCATCAGCTAGATGGGCATATCCTGACACTGGAAGATCCTATTGAG
TTAGTCCATCAATCAAAGCGCTGCCTGATTCAACAACGAGAACCTGGCACACATTGCCGCTCGTTTGCTGCAGCGTTACG
TGCGGCATTGCGTGAAGATCCCGACGTGATTTTATTGGGGGAGTTACGCGATAGCGAAACTATACGCCTGGCGCTCACCG
CTGCGGAAACAGGCCATCTGGTACTGGCAACATTGCATACTCGCGGAGCCGCACAAGCTGTAGAACGGCTGGTGGATGTC
TTTCCTGCGGAAGAAAAAAACATGGTGCGCAGCCAATTGGCCGGCAGTTTAAAAGCGGTGCTGGCGCAGAAATTACAGCT
TGATGCCCAGGGTGGAAGGGTGGCTTTGTTCGAGTTGTTGGTGAATACACCAGCCGTAGCGAGCCTTATCCGTGAAGGGA
AAGCTCATCAGTTGCCCGGGCAGATTCAAACCGGGCAGCAATATGGCATGCAGAGTTTTTCGCACAGCCAAGCGCGGTTA
CGTGCGGAAGGTCGTATTGGTATTGCTCCCTCTCCTGGGGGAGAGGGCTGGGGTGAGGGATATTCCGATGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.453

96.221

0.485
  pilT Vibrio cholerae strain A1552

50.453

96.221

0.485
  pilT Pseudomonas aeruginosa PAK

49.544

95.64

0.474
  pilT Neisseria meningitidis 8013

48.943

96.221

0.471
  pilT Pseudomonas stutzeri DSM 10701

49.24

95.64

0.471
  pilT Neisseria gonorrhoeae MS11

48.64

96.221

0.468
  pilT Acinetobacter baylyi ADP1

48.036

96.221

0.462
  pilT Acinetobacter baumannii D1279779

48.328

95.64

0.462
  pilT Acinetobacter baumannii strain A118

48.328

95.64

0.462
  pilT Legionella pneumophila strain ERS1305867

50.159

91.57

0.459
  pilT Legionella pneumophila strain Lp02

50.159

91.57

0.459
  pilT Acinetobacter nosocomialis M2

48.024

95.64

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.268

91.279

0.404
  pilU Vibrio cholerae strain A1552

39.577

96.221

0.381
  pilU Pseudomonas stutzeri DSM 10701

38.855

96.512

0.375