Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MNQ95_RS10185 Genome accession   NZ_CP093331
Coordinates   2255627..2256025 (+) Length   132 a.a.
NCBI ID   WP_277884371.1    Uniprot ID   -
Organism   Pseudoxanthomonas daejeonensis strain S9-A64R     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2250627..2261025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNQ95_RS10170 (MNQ95_10170) coaE 2251390..2252007 (-) 618 WP_241952796.1 dephospho-CoA kinase -
  MNQ95_RS10175 (MNQ95_10175) - 2252569..2253432 (-) 864 WP_241952797.1 A24 family peptidase -
  MNQ95_RS10180 (MNQ95_10180) pilC 2253451..2254710 (-) 1260 WP_241952798.1 type II secretion system F family protein Machinery gene
  MNQ95_RS10185 (MNQ95_10185) pilA 2255627..2256025 (+) 399 WP_277884371.1 pilin Machinery gene
  MNQ95_RS10190 (MNQ95_10190) pilB 2256275..2258011 (+) 1737 WP_241952799.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MNQ95_RS10195 (MNQ95_10195) - 2259152..2259643 (+) 492 WP_241952800.1 transposase -
  MNQ95_RS10200 (MNQ95_10200) ybgC 2260439..2260849 (+) 411 WP_162408336.1 tol-pal system-associated acyl-CoA thioesterase -

Sequence

Protein


Download         Length: 132 a.a.        Molecular weight: 13437.56 Da        Isoelectric Point: 4.2985

>NTDB_id=663677 MNQ95_RS10185 WP_277884371.1 2255627..2256025(+) (pilA) [Pseudoxanthomonas daejeonensis strain S9-A64R]
MKKNMQGFTLIELMIVVAIIAILAAIALPAYQDYMARARVSEGLVIASGAKATVGENLAADQAPCLGVDGGVINRTTLAC
TAADGALTVAVDAGPATVNLTLTPDLTDTGTTWACAVDAAANNKYVPAECRV

Nucleotide


Download         Length: 399 bp        

>NTDB_id=663677 MNQ95_RS10185 WP_277884371.1 2255627..2256025(+) (pilA) [Pseudoxanthomonas daejeonensis strain S9-A64R]
ATGAAGAAGAACATGCAGGGCTTCACCCTCATCGAACTGATGATCGTCGTGGCGATCATCGCCATCCTGGCCGCCATCGC
GCTGCCGGCCTACCAGGACTACATGGCCCGCGCACGCGTGTCCGAGGGTCTGGTCATCGCTTCCGGCGCCAAGGCGACGG
TCGGTGAGAACCTGGCTGCCGATCAGGCGCCATGCCTGGGTGTGGATGGTGGTGTTATCAACCGCACCACGCTGGCTTGT
ACTGCTGCAGATGGCGCCCTTACCGTTGCGGTCGATGCCGGTCCGGCCACTGTCAACCTGACCCTTACCCCGGATCTGAC
TGATACGGGTACGACTTGGGCCTGCGCGGTTGATGCCGCGGCCAACAACAAGTATGTGCCGGCTGAATGCCGCGTCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-131)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

40.994

100

0.5
  comP Acinetobacter baylyi ADP1

44.828

100

0.492
  pilA/pilA1 Eikenella corrodens VA1

40.26

100

0.47
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.379

100

0.455
  pilA2 Legionella pneumophila str. Paris

44.444

100

0.455
  pilA2 Legionella pneumophila strain ERS1305867

44.444

100

0.455
  pilA Vibrio campbellii strain DS40M4

39.007

100

0.417
  pilE Neisseria gonorrhoeae strain FA1090

35.065

100

0.409
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.571

100

0.409
  pilA Pseudomonas aeruginosa PAK

34.228

100

0.386
  pilA Vibrio parahaemolyticus RIMD 2210633

38.931

99.242

0.386
  pilA Acinetobacter baumannii strain A118

35.556

100

0.364
  pilA Haemophilus influenzae 86-028NP

41.026

88.636

0.364