Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MNQ95_RS10190 Genome accession   NZ_CP093331
Coordinates   2256275..2258011 (+) Length   578 a.a.
NCBI ID   WP_241952799.1    Uniprot ID   -
Organism   Pseudoxanthomonas daejeonensis strain S9-A64R     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2251275..2263011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNQ95_RS10170 (MNQ95_10170) coaE 2251390..2252007 (-) 618 WP_241952796.1 dephospho-CoA kinase -
  MNQ95_RS10175 (MNQ95_10175) - 2252569..2253432 (-) 864 WP_241952797.1 A24 family peptidase -
  MNQ95_RS10180 (MNQ95_10180) pilC 2253451..2254710 (-) 1260 WP_241952798.1 type II secretion system F family protein Machinery gene
  MNQ95_RS10185 (MNQ95_10185) pilA 2255627..2256025 (+) 399 WP_277884371.1 pilin Machinery gene
  MNQ95_RS10190 (MNQ95_10190) pilB 2256275..2258011 (+) 1737 WP_241952799.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MNQ95_RS10195 (MNQ95_10195) - 2259152..2259643 (+) 492 WP_241952800.1 transposase -
  MNQ95_RS10200 (MNQ95_10200) ybgC 2260439..2260849 (+) 411 WP_162408336.1 tol-pal system-associated acyl-CoA thioesterase -
  MNQ95_RS10205 (MNQ95_10205) tolQ 2261015..2261791 (+) 777 WP_162408335.1 protein TolQ -
  MNQ95_RS10210 (MNQ95_10210) tolR 2261868..2262302 (+) 435 WP_162408334.1 protein TolR -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62529.83 Da        Isoelectric Point: 6.0031

>NTDB_id=663678 MNQ95_RS10190 WP_241952799.1 2256275..2258011(+) (pilB) [Pseudoxanthomonas daejeonensis strain S9-A64R]
MNAVVTANLVGITGIARRLVQDGALDEAGARAAMAQATEAKVPLAQWVAEKKLVAPAQMAAANAVEFGMPLIDATAFDPS
QNAMKLVSEELIQKHHVIPLFKRGNKLFVGTSQPTQTASLAELQFHTNLVVEAILVDEDQIRRTLEQWQNSSGQLGAGLS
GGDDDEGLENLEIGGGDEDAGSESGVDAKGDDTPVVKFVNKVLIDAIRRGASDIHFEPYETDYRVRLRIDGLLKSVAKAP
VKLSPRISARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEEDQQK
LFLDAIQKPYGMVLVTGPTGSGKTVSLYTALGILNDDQRNISTVEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIGVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPFNIISSVTLVIAQRLARRLCSRCKKPSELP
EHALLAEGFTEAQIKAGFTLYEAVGCEECTEGYKGRTGIYQVMPMSEEIQAIVLEGGNAMQIAAAAQKAGIRDLRQSALM
KCRSGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=663678 MNQ95_RS10190 WP_241952799.1 2256275..2258011(+) (pilB) [Pseudoxanthomonas daejeonensis strain S9-A64R]
ATGAATGCAGTGGTCACCGCCAACCTGGTCGGCATCACCGGCATCGCACGACGCCTGGTGCAGGACGGTGCGCTGGACGA
GGCCGGCGCGCGCGCCGCCATGGCCCAGGCCACCGAGGCGAAAGTGCCGCTGGCGCAGTGGGTCGCGGAGAAGAAGCTGG
TGGCCCCCGCGCAGATGGCCGCGGCCAACGCGGTCGAATTCGGCATGCCACTGATCGATGCGACCGCCTTCGACCCCAGC
CAGAACGCGATGAAACTGGTCAGTGAGGAACTGATCCAGAAGCACCACGTGATCCCCCTGTTCAAGCGTGGCAACAAGCT
GTTCGTCGGCACCAGCCAGCCGACCCAGACCGCCTCGCTGGCCGAACTGCAGTTCCACACCAACCTGGTGGTGGAAGCGA
TCCTGGTCGACGAGGATCAGATCCGCCGCACCCTGGAGCAGTGGCAGAACTCCAGCGGGCAGCTGGGTGCCGGCCTGAGC
GGCGGCGACGACGACGAAGGGCTGGAAAACCTGGAGATCGGCGGCGGCGACGAAGATGCCGGCTCCGAATCGGGCGTGGA
CGCCAAGGGCGACGACACGCCCGTGGTCAAGTTCGTCAACAAGGTGCTGATCGACGCGATCCGCCGCGGCGCCTCGGACA
TCCATTTCGAGCCCTACGAAACCGACTACCGGGTGCGCCTGCGCATCGATGGCCTGCTCAAGTCAGTGGCCAAGGCGCCG
GTCAAGCTGAGCCCGCGCATATCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAAAAGCGCGTGCCGCAGGA
CGGCCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTATGAGGAGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCAGAAGCCCTACGGCATGGTCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCCTTGGGCATCCTCAACGACGATCAGCGCAATATTTCCACCGTCGAGGATCCGGTGGAAATCCGCCTGCCCG
GCGTGAATCAGGTGCAGCAGAACGTCAAGCGCGGCATGACCTTCGCCGCGGCCCTGCGCAGCTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGGCGTCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACGCTGCATACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTTCAACATCA
TCTCGTCGGTGACCCTGGTGATCGCCCAGCGCCTGGCGCGACGGCTGTGCAGCCGCTGCAAGAAGCCGTCCGAGCTGCCC
GAGCATGCGCTGCTGGCCGAAGGCTTCACCGAGGCGCAGATCAAGGCCGGCTTCACCCTGTACGAAGCGGTCGGCTGCGA
GGAGTGCACCGAGGGCTACAAGGGCCGTACCGGCATCTACCAGGTGATGCCGATGAGCGAGGAGATCCAGGCCATCGTGC
TGGAAGGCGGCAACGCGATGCAGATCGCCGCCGCGGCCCAGAAGGCCGGTATCCGCGACCTGCGCCAGTCGGCGCTGATG
AAGTGCCGCAGTGGCGTCACCAGCCTGGCCGAGATCAATCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.401

98.27

0.535
  pilB Acinetobacter baylyi ADP1

53.521

98.27

0.526
  pilB Legionella pneumophila strain ERS1305867

52.548

98.443

0.517
  pilF Neisseria gonorrhoeae MS11

48.421

98.616

0.478
  pilB Vibrio cholerae strain A1552

52.14

88.927

0.464
  pilB Vibrio campbellii strain DS40M4

45.326

98.097

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

45.714

96.886

0.443
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.516

100

0.377