Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MNO10_RS21315 Genome accession   NZ_CP093328
Coordinates   4645531..4646553 (+) Length   340 a.a.
NCBI ID   WP_241916956.1    Uniprot ID   -
Organism   Rahnella aceris strain S2-A69     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4640531..4651553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNO10_RS21285 (MNO10_21285) hemW 4641205..4642341 (-) 1137 WP_013576835.1 radical SAM family heme chaperone HemW -
  MNO10_RS21290 (MNO10_21290) - 4642334..4642942 (-) 609 WP_241916955.1 XTP/dITP diphosphatase -
  MNO10_RS21295 (MNO10_21295) yggU 4642964..4643269 (-) 306 WP_013576833.1 DUF167 family protein YggU -
  MNO10_RS21300 (MNO10_21300) - 4643254..4643808 (-) 555 WP_013576832.1 YggT family protein -
  MNO10_RS21305 (MNO10_21305) proC 4643895..4644716 (-) 822 WP_013576831.1 pyrroline-5-carboxylate reductase -
  MNO10_RS21310 (MNO10_21310) - 4644789..4645508 (-) 720 WP_013576830.1 YggS family pyridoxal phosphate-dependent enzyme -
  MNO10_RS21315 (MNO10_21315) pilT 4645531..4646553 (+) 1023 WP_241916956.1 type IV pilus twitching motility protein PilT Machinery gene
  MNO10_RS21320 (MNO10_21320) ruvX 4646544..4646972 (-) 429 WP_013576828.1 Holliday junction resolvase RuvX -
  MNO10_RS21325 (MNO10_21325) - 4646972..4647535 (-) 564 WP_013576827.1 YqgE/AlgH family protein -
  MNO10_RS21330 (MNO10_21330) gshB 4647664..4648614 (-) 951 WP_013576826.1 glutathione synthase -
  MNO10_RS21335 (MNO10_21335) rsmE 4648626..4649357 (-) 732 WP_112150887.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MNO10_RS21340 (MNO10_21340) endA 4649445..4650158 (-) 714 WP_241916957.1 deoxyribonuclease I -
  MNO10_RS21345 (MNO10_21345) - 4650252..4650773 (-) 522 WP_037033267.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37034.58 Da        Isoelectric Point: 6.6850

>NTDB_id=663663 MNO10_RS21315 WP_241916956.1 4645531..4646553(+) (pilT) [Rahnella aceris strain S2-A69]
MDIDGLVGRSVKQNASDLHLCTGYPPVLRIDGELCRCEDLPVVEASQMLGLIHAWLDATEKHTLQLAGQVDKALTLGDGV
RMRLNIFQQLHGLSAALRPVPARCPELAALRVPDILQHVIMQEDGLILVTGATGSGKSTTLAALIDHLNRHAKRHIITLE
DPIELIHTGRQCLIQQRELGLHTRSFGEAVRGALREDPDVLLLGELRDTDSIRLALTAAETGHLVLATLHTRNATQAIDR
LVDVFPAEEKAFVRAQLAGSLRAVIAQKLAVTEEKDGRKAGRVALFEVLTSTPAVCNLIREGKTHQLADILQTGAQAGMQ
TFAQSRQQRQRAGLLAEDQD

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=663663 MNO10_RS21315 WP_241916956.1 4645531..4646553(+) (pilT) [Rahnella aceris strain S2-A69]
ATGGATATTGATGGATTAGTGGGACGTAGTGTAAAGCAAAATGCCTCCGATCTGCACCTTTGTACCGGCTATCCGCCTGT
GTTGCGCATTGACGGGGAATTATGTCGCTGTGAAGATCTGCCGGTGGTGGAGGCTTCGCAGATGCTGGGGCTGATCCATG
CGTGGCTGGATGCCACTGAAAAACATACTTTGCAGCTGGCCGGACAGGTGGATAAAGCGCTGACATTGGGGGATGGCGTG
CGGATGCGGCTCAATATCTTTCAGCAACTGCACGGGTTGTCTGCGGCGCTGAGACCGGTGCCTGCCCGTTGCCCCGAGCT
GGCTGCATTGAGGGTGCCGGATATCTTGCAGCACGTCATTATGCAGGAAGACGGCCTGATTTTGGTCACCGGTGCGACGG
GCAGCGGGAAATCCACCACGCTGGCGGCGCTGATTGATCATCTCAACCGCCATGCGAAGCGCCATATTATTACGCTGGAA
GACCCCATCGAGCTTATCCATACTGGCAGGCAATGTCTGATTCAGCAGCGCGAACTCGGGCTGCATACGCGTTCATTTGG
CGAAGCGGTGCGCGGTGCTCTGCGTGAAGATCCGGATGTATTGTTACTGGGCGAACTGCGTGACACTGACAGTATCCGGC
TGGCGCTGACGGCGGCAGAAACCGGACATCTGGTTCTCGCGACACTGCATACGCGCAACGCCACACAGGCCATTGACCGG
CTGGTTGATGTGTTTCCGGCAGAAGAAAAAGCGTTTGTCCGCGCGCAACTGGCGGGCAGCCTGCGGGCCGTGATCGCGCA
AAAACTGGCGGTGACTGAAGAAAAGGATGGGAGAAAAGCGGGAAGGGTAGCGCTTTTTGAAGTGCTGACCTCCACACCGG
CGGTGTGCAATTTAATTCGTGAAGGGAAAACGCATCAGCTGGCGGATATCCTGCAAACCGGCGCGCAGGCCGGCATGCAG
ACGTTCGCACAAAGCCGTCAGCAACGGCAACGTGCGGGATTACTGGCGGAAGATCAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.305

96.471

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.305

96.471

0.485
  pilT Neisseria meningitidis 8013

47.041

99.412

0.468
  pilT Neisseria gonorrhoeae MS11

46.746

99.412

0.465
  pilT Acinetobacter baylyi ADP1

47.164

98.529

0.465
  pilT Pseudomonas stutzeri DSM 10701

45.723

99.706

0.456
  pilT Pseudomonas aeruginosa PAK

47.826

94.706

0.453
  pilT Acinetobacter baumannii strain A118

47.077

95.588

0.45
  pilT Acinetobacter nosocomialis M2

47.077

95.588

0.45
  pilT Acinetobacter baumannii D1279779

47.077

95.588

0.45
  pilT Legionella pneumophila strain ERS1305867

46.296

95.294

0.441
  pilT Legionella pneumophila strain Lp02

46.296

95.294

0.441
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.236

94.706

0.4
  pilU Vibrio cholerae strain A1552

37.994

96.765

0.368
  pilU Acinetobacter baylyi ADP1

37.015

98.529

0.365