Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MIO91_RS10125 Genome accession   NZ_CP092643
Coordinates   2046918..2047976 (-) Length   352 a.a.
NCBI ID   WP_041254111.1    Uniprot ID   A0A413BJ11
Organism   Agathobacter rectalis strain VPI 0990     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2041918..2052976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIO91_RS10095 - 2042160..2042291 (-) 132 WP_306784100.1 hypothetical protein -
  MIO91_RS10100 (MIO91_10035) - 2042830..2043189 (-) 360 WP_012743025.1 hypothetical protein -
  MIO91_RS10105 (MIO91_10040) - 2043335..2044249 (-) 915 WP_012743026.1 IS3 family transposase -
  MIO91_RS10110 (MIO91_10045) - 2044246..2044530 (-) 285 WP_005612369.1 transposase -
  MIO91_RS10115 (MIO91_10050) - 2044608..2045201 (-) 594 WP_049757237.1 PD-(D/E)XK nuclease family transposase -
  MIO91_RS10120 (MIO91_10055) - 2045592..2046806 (-) 1215 WP_012743029.1 type II secretion system F family protein -
  MIO91_RS10125 (MIO91_10060) pilT 2046918..2047976 (-) 1059 WP_041254111.1 type IV pilus twitching motility protein PilT Machinery gene
  MIO91_RS10130 (MIO91_10065) - 2047990..2049693 (-) 1704 WP_012743031.1 GspE/PulE family protein -
  MIO91_RS10135 (MIO91_10070) - 2049793..2051190 (-) 1398 WP_012743032.1 sodium-dependent transporter -
  MIO91_RS10140 (MIO91_10075) ltrA 2051550..2052944 (-) 1395 WP_012743033.1 group II intron reverse transcriptase/maturase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38709.44 Da        Isoelectric Point: 6.3063

>NTDB_id=659514 MIO91_RS10125 WP_041254111.1 2046918..2047976(-) (pilT) [Agathobacter rectalis strain VPI 0990]
MQIVDILKAAQQQNSSDIHIVPGNPVMLRIDGKLVPQGNQVLTNVETDNLLSPIVPQELKDELKEKGELDFAFSVEGFSR
VRANVFRQRGSYAAALRILSYDVPTPQQLGIPQSVVNLTTKMRGLVLVTGATGSGKSTTLASLIDVIASRDSKNIITLED
PIEYLHSRRRSIVEQREVGKDTLSYAAALRAALRQDPDVILVGEMRDLETISTAITAAETGHLVFSTLHTNSSSDAIDRM
IDVFPPHQQQQIRVQLSGVLEGIVAQHLLPMVNGGRIAAFEVLLANNAIRNLIREAKAFQIPSIIQTSKREGMMMMDDCI
LQYYSQGLIAPETAVAYAHDPVSMASRVHLYY

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=659514 MIO91_RS10125 WP_041254111.1 2046918..2047976(-) (pilT) [Agathobacter rectalis strain VPI 0990]
ATGCAGATAGTAGACATTTTAAAAGCAGCGCAGCAACAGAACTCATCGGATATACATATCGTACCGGGAAATCCGGTTAT
GCTTAGAATTGACGGAAAGCTTGTACCGCAGGGAAATCAGGTTCTTACCAATGTTGAGACGGACAACCTGCTTTCTCCAA
TTGTTCCGCAGGAGCTTAAAGATGAGCTAAAGGAGAAGGGAGAGCTTGATTTTGCTTTTTCGGTGGAGGGCTTTTCAAGA
GTCAGAGCCAATGTGTTCAGGCAGAGAGGTTCATATGCAGCAGCACTTCGTATTCTCTCATATGATGTGCCTACACCGCA
GCAGCTTGGCATACCACAGTCGGTTGTCAATCTTACCACAAAGATGAGAGGACTTGTCCTCGTGACAGGAGCTACAGGAA
GTGGTAAGTCGACCACATTGGCAAGTCTTATAGATGTCATTGCATCAAGAGATTCCAAAAATATAATCACGCTTGAAGAT
CCTATTGAGTACCTGCACTCAAGGCGCCGCTCTATAGTGGAACAGCGAGAGGTTGGAAAGGATACACTCTCATATGCTGC
AGCCCTGAGAGCTGCACTGCGACAGGATCCGGATGTAATACTTGTAGGAGAGATGCGAGATCTTGAAACTATATCTACAG
CTATTACAGCGGCGGAGACAGGACATCTTGTATTTTCTACACTGCATACTAACAGTTCATCAGATGCAATTGACCGTATG
ATTGACGTTTTTCCACCACACCAGCAGCAACAGATACGAGTGCAGCTCTCAGGAGTTTTAGAGGGAATTGTTGCACAGCA
TCTGCTTCCAATGGTAAATGGCGGACGAATTGCAGCATTTGAGGTGCTCCTTGCAAACAATGCCATCAGAAACTTAATTC
GTGAGGCAAAAGCATTTCAGATTCCATCTATCATACAGACATCAAAGAGAGAGGGTATGATGATGATGGATGACTGTATT
TTACAGTATTACAGCCAGGGCCTTATAGCGCCGGAGACTGCAGTTGCATATGCACATGATCCGGTAAGCATGGCTAGCCG
TGTACACCTTTACTATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A413BJ11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.133

98.295

0.483
  pilT Acinetobacter baylyi ADP1

45.058

97.727

0.44
  pilT Legionella pneumophila strain ERS1305867

46.108

94.886

0.438
  pilT Legionella pneumophila strain Lp02

46.108

94.886

0.438
  pilT Vibrio cholerae strain A1552

46.201

93.466

0.432
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.201

93.466

0.432
  pilT Pseudomonas stutzeri DSM 10701

44.282

96.875

0.429
  pilT Acinetobacter nosocomialis M2

43.895

97.727

0.429
  pilT Acinetobacter baumannii D1279779

43.605

97.727

0.426
  pilT Acinetobacter baumannii strain A118

43.605

97.727

0.426
  pilT Pseudomonas aeruginosa PAK

43.988

96.875

0.426
  pilT Neisseria meningitidis 8013

42.775

98.295

0.42
  pilT Neisseria gonorrhoeae MS11

42.486

98.295

0.418
  pilU Vibrio cholerae strain A1552

38.938

96.307

0.375
  pilU Pseudomonas stutzeri DSM 10701

38.053

96.307

0.366