Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MCP04_RS20470 Genome accession   NZ_CP092419
Coordinates   4261592..4262701 (-) Length   369 a.a.
NCBI ID   WP_017288851.1    Uniprot ID   A0AA96WVU7
Organism   Leptolyngbya boryana IU 594     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4256592..4267701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MCP04_RS20445 (MCP04_20445) - 4258405..4258614 (+) 210 WP_017288846.1 DUF2997 domain-containing protein -
  MCP04_RS20450 (MCP04_20450) - 4258657..4259043 (+) 387 WP_017288847.1 DUF1257 domain-containing protein -
  MCP04_RS20455 (MCP04_20455) - 4259044..4259523 (+) 480 WP_017288848.1 ferredoxin -
  MCP04_RS20460 (MCP04_20460) - 4259569..4260147 (-) 579 WP_026148724.1 hypothetical protein -
  MCP04_RS20465 (MCP04_20465) - 4260257..4261480 (-) 1224 WP_017288850.1 type II secretion system F family protein -
  MCP04_RS20470 (MCP04_20470) pilT 4261592..4262701 (-) 1110 WP_017288851.1 type IV pilus twitching motility protein PilT Machinery gene
  MCP04_RS20475 (MCP04_20475) pilB/pilB1 4262755..4264755 (-) 2001 WP_017288852.1 GspE/PulE family protein Machinery gene
  MCP04_RS20480 (MCP04_20480) grpE 4264959..4265642 (+) 684 WP_017288853.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40731.94 Da        Isoelectric Point: 6.9125

>NTDB_id=657348 MCP04_RS20470 WP_017288851.1 4261592..4262701(-) (pilT) [Leptolyngbya boryana IU 594]
MEMMIEDLMEQLIEMGGSDMHLTAGLPPYFRISGHLQPVSDHVLSAEECQRLIFSMLNNTQRKTLEQNWELDCSYGVRGL
ARFRVNVYKDRGTYAACLRALSSKIPNFEKLNLPDVVREMSEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILTI
EDPIEFVYEPIKSLVHQRQLHEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RMIDVFPSERQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRVMAQEIMIVTPAIANLIREGKTAQIYSAIQTGGKLGM
QTLEKVLADQYKAGLITFEAAASKTSRPDELQRLIGGVPNGAAGTAVKR

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=657348 MCP04_RS20470 WP_017288851.1 4261592..4262701(-) (pilT) [Leptolyngbya boryana IU 594]
ATGGAAATGATGATCGAAGACTTGATGGAACAACTCATCGAAATGGGCGGTTCTGACATGCACCTGACCGCAGGACTGCC
GCCTTATTTTCGCATCAGCGGACATCTTCAACCCGTCAGCGATCATGTTCTCAGTGCAGAAGAATGTCAGCGTTTGATTT
TCAGTATGCTGAATAACACTCAGCGCAAAACACTAGAGCAAAACTGGGAGCTTGACTGCTCTTATGGTGTGCGCGGATTA
GCTCGTTTTCGGGTCAATGTCTACAAAGATCGCGGAACTTACGCTGCATGTCTACGGGCACTCAGTTCCAAAATTCCAAA
TTTTGAAAAACTGAATCTTCCCGATGTCGTTCGAGAAATGTCCGAAAAGCCAAGAGGCTTAATCCTAGTTACAGGTCCTA
CCGGTTCAGGTAAGACAACGACTTTAGCTGCAATGATTGATTTGATCAACCGCACTCGTGCAGAACATATTTTGACGATC
GAAGATCCGATCGAATTTGTATACGAACCTATCAAAAGTTTGGTTCACCAACGCCAGCTTCATGAAGATACAAAGAGCTT
TGCAAATGCTCTAAAAGCAGCTCTCCGGGAAGATCCAGATATTATTCTGGTTGGGGAAATGCGGGACTTGGAAACTATCT
CACTAGCAATTTCTGCGGCAGAAACAGGTCACTTAGTTTTCGGCACGCTCCATACGAGTTCTGCGGCACAAACCGTTGAC
CGGATGATTGATGTCTTTCCATCCGAGCGTCAAACGCAAGTGCGGGTGCAGCTTTCCAACTCACTCGTTGCAGTCTTCAG
CCAAACGCTGGTGCCGAAGAAGAATCCCAAACCGGGTGAATATGGGCGAGTCATGGCTCAGGAAATTATGATTGTCACCC
CTGCGATCGCCAACCTGATTCGTGAAGGGAAAACCGCCCAAATTTACTCAGCAATTCAGACAGGCGGAAAATTGGGAATG
CAAACCCTTGAAAAAGTGCTAGCTGATCAATACAAAGCTGGACTGATTACATTTGAAGCCGCCGCCTCCAAAACTTCTCG
TCCTGATGAACTTCAGCGCTTAATTGGTGGAGTTCCGAATGGTGCAGCAGGCACAGCAGTGAAGCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.441

94.58

0.515
  pilT Pseudomonas stutzeri DSM 10701

53.582

94.58

0.507
  pilT Acinetobacter baylyi ADP1

52.149

94.58

0.493
  pilT Legionella pneumophila strain ERS1305867

52.161

94.038

0.491
  pilT Legionella pneumophila strain Lp02

52.161

94.038

0.491
  pilT Acinetobacter nosocomialis M2

51.576

94.58

0.488
  pilT Acinetobacter baumannii D1279779

51.576

94.58

0.488
  pilT Acinetobacter baumannii strain A118

51.576

94.58

0.488
  pilT Neisseria gonorrhoeae MS11

51.622

91.87

0.474
  pilT Neisseria meningitidis 8013

51.327

91.87

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.383

98.374

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

92.683

0.458
  pilT Vibrio cholerae strain A1552

49.415

92.683

0.458
  pilU Pseudomonas stutzeri DSM 10701

39.295

100

0.393