Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   MCP04_RS20475 Genome accession   NZ_CP092419
Coordinates   4262755..4264755 (-) Length   666 a.a.
NCBI ID   WP_017288852.1    Uniprot ID   A0AA96WVH0
Organism   Leptolyngbya boryana IU 594     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4257755..4269755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MCP04_RS20445 (MCP04_20445) - 4258405..4258614 (+) 210 WP_017288846.1 DUF2997 domain-containing protein -
  MCP04_RS20450 (MCP04_20450) - 4258657..4259043 (+) 387 WP_017288847.1 DUF1257 domain-containing protein -
  MCP04_RS20455 (MCP04_20455) - 4259044..4259523 (+) 480 WP_017288848.1 ferredoxin -
  MCP04_RS20460 (MCP04_20460) - 4259569..4260147 (-) 579 WP_026148724.1 hypothetical protein -
  MCP04_RS20465 (MCP04_20465) - 4260257..4261480 (-) 1224 WP_017288850.1 type II secretion system F family protein -
  MCP04_RS20470 (MCP04_20470) pilT 4261592..4262701 (-) 1110 WP_017288851.1 type IV pilus twitching motility protein PilT Machinery gene
  MCP04_RS20475 (MCP04_20475) pilB/pilB1 4262755..4264755 (-) 2001 WP_017288852.1 GspE/PulE family protein Machinery gene
  MCP04_RS20480 (MCP04_20480) grpE 4264959..4265642 (+) 684 WP_017288853.1 nucleotide exchange factor GrpE -
  MCP04_RS20485 (MCP04_20485) dnaK 4265827..4267776 (+) 1950 WP_026148725.1 molecular chaperone DnaK -
  MCP04_RS20490 (MCP04_20490) dnaJ 4267873..4269009 (+) 1137 WP_017288855.1 molecular chaperone DnaJ -
  MCP04_RS20495 (MCP04_20495) - 4269006..4269239 (+) 234 WP_017288856.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 666 a.a.        Molecular weight: 74222.09 Da        Isoelectric Point: 4.9046

>NTDB_id=657349 MCP04_RS20475 WP_017288852.1 4262755..4264755(-) (pilB/pilB1) [Leptolyngbya boryana IU 594]
MTNSSSQRRALVVQNNFSPFGNKLIQSGYVNNEQMQQALIECRKSGRPLTAVLEALTGQQLSPDLIRQYKRQQLFELKIL
YGVESLDPELNQVSSAQVGQLIDTLIPIELCRRYNLIPLSQSETDPPFVLVAMVDPDNLAAQDELNRILRPKGIGLQRLV
ITIDDYQRLISTYMDARLEQEKQLEAQSRVDVRSDLEGLESLAELSEDDSAEMDLDAGAEADAAPIIALVNKILIKALQM
AVSDIHIEPQEESLRIRFRKDGVLREAFEPFPKKIIPAVVARFKIISQLDIAERRAPQDGRIRRIYDGRKVDFRVNTLPS
RYGEKVVLRILDNSATQLGLDKLITDETSLRIVQEMVKRPFGLMLVTGPTGSGKTTTLYSALAERNEPGVNISTAEDPIE
YSLPGITQVQVIREKGMDFAAILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDEMGVE
PFMVSGALLGVVAQRLVRKVCDECRIPYQPSPEELARFGLTSAGDAQLTLYKANTLSPQEIQEARSKNQLCQKCSGVGYK
GRLGVYEVMRVTEALQTLITEGAPTERIKEVAVEEGMQTLLAYSLDLVRKGLCTLEEIERVTFTDTGLEAELKAKRKTSL
TCRTCGAEMQQDWLDCHYCTTPRFQD

Nucleotide


Download         Length: 2001 bp        

>NTDB_id=657349 MCP04_RS20475 WP_017288852.1 4262755..4264755(-) (pilB/pilB1) [Leptolyngbya boryana IU 594]
ATGACAAACTCTTCTTCTCAGCGGCGCGCCCTTGTTGTTCAAAACAATTTTTCGCCGTTTGGCAATAAATTGATTCAATC
TGGCTACGTCAATAACGAGCAAATGCAGCAGGCTCTGATTGAATGTCGAAAGTCAGGTAGACCGCTGACTGCTGTGCTAG
AAGCGCTGACCGGGCAGCAGCTGTCACCGGATTTGATTCGCCAATATAAACGGCAGCAACTGTTTGAGTTGAAGATTCTT
TACGGCGTTGAATCTCTTGATCCTGAACTCAATCAGGTTTCTTCCGCTCAAGTCGGGCAGTTAATCGACACGTTGATTCC
AATTGAGCTTTGCCGTCGTTATAATCTCATTCCCCTTTCTCAAAGCGAGACCGATCCACCTTTTGTGCTTGTGGCAATGG
TGGATCCCGACAATCTAGCAGCGCAGGATGAATTAAATCGCATCTTACGTCCAAAAGGCATTGGATTGCAGCGATTAGTC
ATTACGATCGATGATTATCAGCGGCTGATTTCTACCTACATGGATGCTCGGCTTGAGCAGGAGAAGCAGCTTGAAGCCCA
ATCTAGAGTAGATGTGCGCTCAGATCTCGAAGGGTTAGAATCCCTTGCCGAGCTATCAGAAGATGACAGCGCAGAAATGG
ATCTCGATGCTGGTGCTGAGGCTGATGCCGCTCCGATCATTGCGCTGGTGAATAAAATTCTGATTAAAGCACTGCAAATG
GCAGTCTCTGATATTCACATCGAACCGCAAGAAGAATCTCTCCGCATCCGATTTCGTAAAGATGGAGTCTTACGAGAAGC
GTTTGAACCTTTTCCGAAGAAGATTATCCCCGCAGTTGTAGCACGCTTCAAAATTATTTCTCAACTCGATATCGCAGAAC
GTCGAGCGCCGCAGGATGGCCGGATTCGCCGGATTTATGACGGTCGCAAAGTAGACTTCCGAGTCAACACACTCCCCAGT
CGCTATGGCGAAAAAGTTGTCCTTCGGATTCTAGATAATTCGGCGACACAGCTTGGACTCGATAAGTTGATTACGGACGA
AACTTCTTTGAGAATCGTTCAAGAAATGGTGAAACGTCCTTTCGGTCTCATGTTAGTAACTGGCCCCACGGGGTCCGGAA
AAACCACAACACTGTACTCAGCTTTGGCTGAACGTAACGAACCTGGAGTCAACATTAGTACGGCTGAAGACCCGATCGAG
TATTCTCTCCCTGGAATTACCCAAGTTCAGGTCATCCGGGAAAAAGGGATGGACTTCGCTGCAATTTTGAGAGCGTTCCT
ACGACAAGATCCGGACGTCATTCTGGTCGGTGAGACCCGCGACAAAGAAACAGCAAAAACCGCGATCGAAGCTGCTCTGA
CGGGTCACTTGGTTTTGACCACTCTCCACACCAATGATGCCGCAGGTGCGATCGCTCGACTCGATGAAATGGGAGTTGAA
CCCTTCATGGTGTCCGGCGCATTGCTGGGTGTTGTTGCTCAGCGTTTGGTTCGGAAAGTCTGTGACGAATGCCGAATCCC
TTACCAACCTTCCCCCGAAGAACTTGCTCGCTTCGGATTAACCAGTGCGGGTGATGCCCAACTGACGTTATATAAAGCCA
ACACTTTAAGTCCCCAGGAAATTCAGGAAGCTCGCTCGAAAAATCAACTTTGTCAGAAATGTAGCGGGGTTGGTTATAAA
GGACGACTCGGGGTTTATGAAGTGATGCGAGTAACAGAAGCGCTTCAGACCTTAATTACAGAGGGCGCTCCGACTGAACG
AATTAAAGAAGTAGCTGTGGAAGAAGGAATGCAAACCCTACTCGCTTACAGCTTGGATCTTGTTCGCAAAGGACTTTGCA
CGCTTGAAGAAATTGAGCGGGTCACCTTCACTGACACTGGACTCGAAGCCGAACTCAAGGCAAAGCGTAAGACTTCGCTC
ACCTGCCGGACTTGTGGAGCAGAAATGCAGCAGGATTGGCTCGACTGCCACTATTGCACGACTCCGCGCTTCCAAGATTA
A

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(70-179)

Type II/IV secretion system protein (T2SSE)

(232-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

68.271

99.85

0.682
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.516

93.093

0.368