Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MF259_RS18870 Genome accession   NZ_CP092258
Coordinates   3904661..3905641 (-) Length   326 a.a.
NCBI ID   WP_023226760.1    Uniprot ID   A0A4Y6MYX5
Organism   Salmonella enterica subsp. enterica serovar Indiana strain 15     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3899661..3910641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MF259_RS18840 (MF259_18840) endA 3900121..3900828 (+) 708 WP_001650639.1 deoxyribonuclease I -
  MF259_RS18845 (MF259_18845) rsmE 3900905..3901636 (+) 732 WP_001222489.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MF259_RS18850 (MF259_18850) gshB 3901656..3902603 (+) 948 WP_000593248.1 glutathione synthase -
  MF259_RS18855 (MF259_18855) - 3902819..3903382 (+) 564 WP_001053167.1 YqgE/AlgH family protein -
  MF259_RS18860 (MF259_18860) ruvX 3903382..3903798 (+) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  MF259_RS18865 (MF259_18865) - 3903845..3904531 (-) 687 WP_023226761.1 IclR family transcriptional regulator -
  MF259_RS18870 (MF259_18870) pilT 3904661..3905641 (-) 981 WP_023226760.1 type IV pilus twitching motility protein PilT Machinery gene
  MF259_RS18875 (MF259_18875) - 3905659..3906363 (+) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  MF259_RS18880 (MF259_18880) - 3906382..3906948 (+) 567 WP_001094848.1 YggT family protein -
  MF259_RS18885 (MF259_18885) yggU 3906945..3907235 (+) 291 WP_001277203.1 DUF167 family protein YggU -
  MF259_RS18890 (MF259_18890) - 3907243..3907836 (+) 594 WP_001174773.1 XTP/dITP diphosphatase -
  MF259_RS18895 (MF259_18895) hemW 3907829..3908965 (+) 1137 WP_001096530.1 radical SAM family heme chaperone HemW -
  MF259_RS18900 (MF259_18900) - 3909057..3910064 (-) 1008 WP_023226759.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36169.34 Da        Isoelectric Point: 7.5355

>NTDB_id=656370 MF259_RS18870 WP_023226760.1 3904661..3905641(-) (pilT) [Salmonella enterica subsp. enterica serovar Indiana strain 15]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRKHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHEIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=656370 MF259_RS18870 WP_023226760.1 3904661..3905641(-) (pilT) [Salmonella enterica subsp. enterica serovar Indiana strain 15]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAAAGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCAGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGAGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y6MYX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

35.65

100

0.362