Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MC377_RS03590 Genome accession   NZ_CP092075
Coordinates   742064..743044 (+) Length   326 a.a.
NCBI ID   WP_001055651.1    Uniprot ID   -
Organism   Salmonella enterica strain P281972     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 737064..748044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MC377_RS03560 (MC377_03560) - 737641..738648 (+) 1008 WP_000252205.1 DUF1202 family protein -
  MC377_RS03565 (MC377_03565) hemW 738740..739876 (-) 1137 WP_001096532.1 radical SAM family heme chaperone HemW -
  MC377_RS03570 (MC377_03570) - 739869..740462 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  MC377_RS03575 (MC377_03575) yggU 740470..740760 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  MC377_RS03580 (MC377_03580) - 740757..741323 (-) 567 WP_001094848.1 YggT family protein -
  MC377_RS03585 (MC377_03585) - 741342..742046 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  MC377_RS03590 (MC377_03590) pilT 742064..743044 (+) 981 WP_001055651.1 type IV pilus twitching motility protein PilT Machinery gene
  MC377_RS03595 (MC377_03595) - 743174..743860 (+) 687 WP_000098335.1 IclR family transcriptional regulator -
  MC377_RS03600 (MC377_03600) ruvX 743907..744323 (-) 417 WP_000099435.1 Holliday junction resolvase RuvX -
  MC377_RS03605 (MC377_03605) - 744323..744886 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  MC377_RS03610 (MC377_03610) gshB 745102..746049 (-) 948 WP_000593248.1 glutathione synthase -
  MC377_RS03615 (MC377_03615) rsmE 746069..746800 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MC377_RS03620 (MC377_03620) endA 746877..747584 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36166.42 Da        Isoelectric Point: 8.1066

>NTDB_id=655807 MC377_RS03590 WP_001055651.1 742064..743044(+) (pilT) [Salmonella enterica strain P281972]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRKHGQLDFAVSLSGTRRLRA
SAFKHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=655807 MC377_RS03590 WP_001055651.1 742064..743044(+) (pilT) [Salmonella enterica strain P281972]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAAAGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCAAACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCCACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCTGCCGGACTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGCGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAAGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

35.65

100

0.362