Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L6494_RS13465 Genome accession   NZ_CP091913
Coordinates   3187192..3188310 (-) Length   372 a.a.
NCBI ID   WP_237995692.1    Uniprot ID   -
Organism   Nostoc sp. UHCC 0870     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3182192..3193310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6494_RS13440 (L6494_13435) - 3183526..3183771 (-) 246 WP_237995681.1 hypothetical protein -
  L6494_RS13445 (L6494_13440) - 3183761..3184327 (-) 567 WP_237995684.1 DUF2808 domain-containing protein -
  L6494_RS13450 (L6494_13445) - 3184536..3185024 (+) 489 WP_237995686.1 hypothetical protein -
  L6494_RS13455 (L6494_13450) - 3185162..3185719 (-) 558 WP_237995688.1 hypothetical protein -
  L6494_RS13460 (L6494_13455) - 3185888..3187111 (-) 1224 WP_237995690.1 type II secretion system F family protein -
  L6494_RS13465 (L6494_13460) pilT 3187192..3188310 (-) 1119 WP_237995692.1 type IV pilus twitching motility protein PilT Machinery gene
  L6494_RS13470 (L6494_13465) pilB/pilB1 3188442..3190454 (-) 2013 WP_237995694.1 GspE/PulE family protein Machinery gene
  L6494_RS13475 (L6494_13470) grpE 3190792..3191538 (+) 747 WP_237995695.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 40832.25 Da        Isoelectric Point: 7.4479

>NTDB_id=654647 L6494_RS13465 WP_237995692.1 3187192..3188310(-) (pilT) [Nostoc sp. UHCC 0870]
MEMMIEDLMEQLIEMGGSDMHLSAGLPPYFRISGKLTPIGEHILTSEQCQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDVVREMCDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSASQTVD
RIIDVFPHEKQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRVMAQEIMIITPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYKSGMISFEAAMSKTSKPDEIQRLIGASAPPPGAAKAGAKAH

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=654647 L6494_RS13465 WP_237995692.1 3187192..3188310(-) (pilT) [Nostoc sp. UHCC 0870]
ATGGAAATGATGATTGAAGACTTGATGGAGCAGTTGATTGAAATGGGTGGCTCGGATATGCACTTATCCGCAGGTTTGCC
TCCCTATTTTCGCATCAGTGGCAAGCTCACACCCATTGGTGAGCATATATTGACCTCAGAACAGTGTCAAAGACTGATTT
TTAGTATGCTCAACAATACCCAGCGTAAAACCCTAGAACAAACCTGGGAGTTAGATTGTTCCTATGGAGTGAAAGGTTTA
GCACGTTTTCGGGTGAATGTTTATAAAGAGCGTGGTGCTTATGCTGCTTGTTTAAGAGCGCTCAGTTCCAAAATTCCTAA
TTTTGAAAAATTAGGTCTGCCAGATGTTGTTAGAGAAATGTGTGATAAACCCAGAGGATTGATTCTGGTGACAGGCCCTA
CAGGTTCTGGTAAAACAACGACCCTAGCGGCAATGATCGACTTGATTAATCGCACCAAAGCAGAGCATATTCTCACAGTA
GAAGACCCTATTGAATTTGTCTATGAACCAATTAAAAGTCTAGTTCACCAACGACAATTGGGTGAAGATACGAAGAGTTT
TGCTAATGCCTTGAAAGCAGCATTACGGGAAGACCCAGATATTGTTCTGGTGGGGGAAATGCGGGATTTAGAAACTATTT
CCTTGGCAATTTCCGCAGCAGAAACAGGTCACTTAGTATTTGGTACGCTACACACTAGTTCAGCTTCTCAGACTGTTGAC
CGGATTATTGACGTTTTCCCTCACGAAAAACAAACTCAAGTGAGGGTGCAATTATCTAACTCTTTAGTAGCAGTATTTAG
TCAAACATTAGTGCCGAAGAAAAACCCCAAACCAGGTGAATATGGTCGGGTTATGGCACAAGAAATTATGATTATTACTC
CCGCTATTTCTAACTTAATTCGTGAAGGTAAAACTTCGCAAATTTATTCGGCTATTCAGACTGGCGGTAAATTAGGAATG
CAAACTTTAGAGAAGGTTTTAGCTGATTATTACAAATCGGGCATGATTTCTTTTGAAGCGGCCATGTCTAAAACCTCTAA
ACCAGACGAGATTCAACGCCTCATCGGTGCATCTGCTCCACCACCAGGCGCAGCTAAAGCTGGTGCAAAAGCCCATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.441

93.817

0.511
  pilT Pseudomonas stutzeri DSM 10701

53.868

93.817

0.505
  pilT Legionella pneumophila strain Lp02

52.738

93.28

0.492
  pilT Acinetobacter baylyi ADP1

52.738

93.28

0.492
  pilT Legionella pneumophila strain ERS1305867

52.738

93.28

0.492
  pilT Acinetobacter baumannii strain A118

52.45

93.28

0.489
  pilT Acinetobacter nosocomialis M2

52.45

93.28

0.489
  pilT Acinetobacter baumannii D1279779

52.45

93.28

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.368

97.043

0.46
  pilT Neisseria gonorrhoeae MS11

49.558

91.129

0.452
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.123

91.935

0.452
  pilT Vibrio cholerae strain A1552

49.123

91.935

0.452
  pilT Neisseria meningitidis 8013

47.851

93.817

0.449
  pilU Pseudomonas stutzeri DSM 10701

37.119

97.043

0.36