Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   L6494_RS13470 Genome accession   NZ_CP091913
Coordinates   3188442..3190454 (-) Length   670 a.a.
NCBI ID   WP_237995694.1    Uniprot ID   -
Organism   Nostoc sp. UHCC 0870     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3183442..3195454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6494_RS13440 (L6494_13435) - 3183526..3183771 (-) 246 WP_237995681.1 hypothetical protein -
  L6494_RS13445 (L6494_13440) - 3183761..3184327 (-) 567 WP_237995684.1 DUF2808 domain-containing protein -
  L6494_RS13450 (L6494_13445) - 3184536..3185024 (+) 489 WP_237995686.1 hypothetical protein -
  L6494_RS13455 (L6494_13450) - 3185162..3185719 (-) 558 WP_237995688.1 hypothetical protein -
  L6494_RS13460 (L6494_13455) - 3185888..3187111 (-) 1224 WP_237995690.1 type II secretion system F family protein -
  L6494_RS13465 (L6494_13460) pilT 3187192..3188310 (-) 1119 WP_237995692.1 type IV pilus twitching motility protein PilT Machinery gene
  L6494_RS13470 (L6494_13465) pilB/pilB1 3188442..3190454 (-) 2013 WP_237995694.1 GspE/PulE family protein Machinery gene
  L6494_RS13475 (L6494_13470) grpE 3190792..3191538 (+) 747 WP_237995695.1 nucleotide exchange factor GrpE -
  L6494_RS13480 (L6494_13475) dnaK 3191759..3193720 (+) 1962 WP_237995697.1 molecular chaperone DnaK -
  L6494_RS13485 (L6494_13480) dnaJ 3194285..3195409 (+) 1125 WP_237995700.1 molecular chaperone DnaJ -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74936.81 Da        Isoelectric Point: 5.0508

>NTDB_id=654648 L6494_RS13470 WP_237995694.1 3188442..3190454(-) (pilB/pilB1) [Nostoc sp. UHCC 0870]
MTQSSPQRRSTALTTRTEFSPFGNKLVQAGYVNNDQMRQALIESRKSGRPLTEVLESITGRQLSPEFLRQYKKQQLFELK
ILYGVEFLDPEVNNVGDTIMGELIETLIPVDICRRHRLVPLLKNDQQNPPCILVAMVDPDNLEASDDLNRILRPQGLALK
RMVITQDDYQQLINQYLDDLAVRQKHIEQEKFTDINQDLENLSNLNLEDAPEEMEADLGAAMKGAEDAPVINLVNRILAK
ALHEQVSDIHVEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLQIVQDMVSKPFGLILVTGPTGSGKTTSLYSALSEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCGDCRIAYTPTPEELARYGMSASQDVGVTFYKANTLSLEAIAEAKAKNNLCPTCNG
VGYKGRCGVYEVMRVTEQLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGSTTLEEVERVTFTDTGLEAELKAKR
KSGLTCRNCNAVLQPEWLDCPYCMTPRFDD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=654648 L6494_RS13470 WP_237995694.1 3188442..3190454(-) (pilB/pilB1) [Nostoc sp. UHCC 0870]
ATGACTCAATCGTCTCCGCAACGGCGTAGTACCGCTCTCACTACAAGAACAGAGTTTTCGCCTTTTGGTAACAAGCTAGT
CCAAGCTGGCTATGTTAATAATGACCAAATGAGGCAGGCGTTGATTGAAAGTCGCAAATCTGGTAGACCCTTAACGGAAG
TGCTGGAGTCAATTACCGGGCGACAACTATCACCTGAGTTCCTCAGACAATACAAGAAACAACAATTATTTGAACTGAAA
ATACTATACGGTGTTGAATTCCTCGATCCGGAAGTCAACAACGTCGGCGATACGATTATGGGGGAACTTATAGAAACCCT
CATCCCAGTTGATATCTGCCGTCGCCATCGTTTAGTACCACTATTGAAGAATGATCAACAAAATCCACCCTGCATTTTAG
TAGCGATGGTCGATCCGGACAACTTAGAAGCGTCCGATGACCTCAACCGGATCTTGCGCCCACAAGGTTTGGCATTAAAG
CGCATGGTGATTACCCAGGATGACTACCAACAGCTGATCAACCAATATCTGGATGACTTGGCTGTGCGACAAAAGCATAT
AGAACAAGAAAAGTTTACAGATATTAATCAGGATTTAGAAAATCTTAGCAATCTTAACCTAGAAGATGCTCCTGAAGAGA
TGGAGGCGGATTTAGGGGCGGCGATGAAGGGTGCAGAAGATGCGCCAGTTATCAACCTCGTTAATAGAATTTTGGCAAAG
GCTCTACACGAGCAGGTTTCTGATATCCACGTCGAACCGCAAGAAGAAAATCTGCGGATTCGCTTCCGTAAGGATGGGGT
GTTGCGGGAGGCTTTTGACCCCCTCCCAAAAAAAATCATTCCCGCAGTTACAGCCAGATTTAAAATTATCTCTAACTTGG
ATATTGCTGAGAGACGTTTACCCCAAGACGGACGTATCAGGCGGATGTTCGAGGGACGGAAAGTAGACTTCCGGGTGAAT
ACCTTGCCCAGCCGCTACGGGGAAAAGGTCGTACTGCGGATTTTAGATAACTCTTCTACCCAACTGGGATTAAATAAGTT
AATTACCGACCCAGAAACTTTGCAGATTGTCCAAGATATGGTCAGTAAGCCTTTTGGGTTAATTTTGGTGACAGGGCCTA
CTGGTTCTGGTAAAACAACTTCCCTATATTCAGCATTATCAGAAAAGAACTCTCCCGGTATTAATATCAGTACGGTAGAA
GACCCGATTGAGTACAGCTTGCCGGGGATTACCCAAGTACAGGTAATTCGGGAAAAAGGACTAGATTTTGCTACAGCATT
GCGGGCATTCTTGCGACAAGACCCAGATGTGTTACTGGTGGGTGAAACAAGAGATAAAGAAACAGCTAAAACAGCGATTG
AGGCGGCTTTAACTGGTCACTTAGTATTAACTACCTTACATACTAACGATGCCCCAGGTGCGATCGCGCGTTTGGGTGAA
ATGGGCATTGAGCCGTTCATGGTTTCTAGTTCGTTAATTGGGGTATTAGCACAACGTTTGGTGCGGCGTGTGTGTGGTGA
TTGTCGCATTGCTTATACTCCTACACCTGAAGAACTGGCTCGTTATGGAATGTCAGCTTCCCAAGATGTGGGGGTGACTT
TCTATAAGGCTAATACTTTATCACTAGAAGCGATCGCTGAAGCTAAAGCTAAAAATAATCTCTGTCCCACCTGTAATGGT
GTCGGTTACAAAGGGCGTTGTGGTGTTTATGAAGTAATGCGGGTAACTGAACAGCTACAAACTTTAATCAACGAAGAAGC
ACCCACAGAACGCATTAAGGAAGTAGCAGTAGAAGAAGGGATGAAAACCTTGTTGGCTTACAGCCTAGACTTAGTTCGCC
AAGGCTCTACAACCCTAGAAGAAGTAGAACGGGTGACGTTTACTGACACAGGTTTAGAAGCTGAGTTAAAAGCCAAGCGC
AAGAGTGGACTAACCTGTCGAAATTGTAATGCTGTATTACAACCAGAATGGCTAGATTGTCCCTACTGTATGACACCTCG
GTTTGATGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-179)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.875

100

0.663
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.725

95.97

0.372