Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L8I56_RS04005 Genome accession   NZ_CP091878
Coordinates   824551..825531 (+) Length   326 a.a.
NCBI ID   WP_001055660.1    Uniprot ID   A0A3G3DWC9
Organism   Salmonella enterica subsp. enterica serovar Tennessee strain 21-SA00318-0     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 819551..830531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L8I56_RS03975 (L8I56_03975) - 820129..821136 (+) 1008 WP_000252197.1 DUF1202 domain-containing protein -
  L8I56_RS03980 (L8I56_03980) hemW 821227..822363 (-) 1137 WP_001096521.1 radical SAM family heme chaperone HemW -
  L8I56_RS03985 (L8I56_03985) - 822356..822949 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  L8I56_RS03990 (L8I56_03990) yggU 822957..823247 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  L8I56_RS03995 (L8I56_03995) - 823244..823810 (-) 567 WP_001094848.1 YggT family protein -
  L8I56_RS04000 (L8I56_04000) - 823829..824533 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  L8I56_RS04005 (L8I56_04005) pilT 824551..825531 (+) 981 WP_001055660.1 type IV pilus twitching motility protein PilT Machinery gene
  L8I56_RS04010 (L8I56_04010) - 825662..826348 (+) 687 WP_000098336.1 IclR family transcriptional regulator -
  L8I56_RS04015 (L8I56_04015) ruvX 826395..826811 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  L8I56_RS04020 (L8I56_04020) - 826811..827374 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  L8I56_RS04025 (L8I56_04025) gshB 827590..828537 (-) 948 WP_000593245.1 glutathione synthase -
  L8I56_RS04030 (L8I56_04030) rsmE 828557..829288 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L8I56_RS04035 (L8I56_04035) endA 829365..830072 (-) 708 WP_000286133.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36126.31 Da        Isoelectric Point: 7.5356

>NTDB_id=654156 L8I56_RS04005 WP_001055660.1 824551..825531(+) (pilT) [Salmonella enterica subsp. enterica serovar Tennessee strain 21-SA00318-0]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQEGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=654156 L8I56_RS04005 WP_001055660.1 824551..825531(+) (pilT) [Salmonella enterica subsp. enterica serovar Tennessee strain 21-SA00318-0]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACAGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGTCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTACTGGGAGAGTTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGAGGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G3DWC9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362