Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L6Y89_RS18130 Genome accession   NZ_CP091779
Coordinates   3845774..3846754 (-) Length   326 a.a.
NCBI ID   WP_237844826.1    Uniprot ID   -
Organism   Enterobacter mori strain ACYC.E9L     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3840774..3851754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6Y89_RS18100 (L6Y89_18100) - 3841663..3842160 (+) 498 WP_010435446.1 SprT family zinc-dependent metalloprotease -
  L6Y89_RS18105 (L6Y89_18105) endA 3842255..3842962 (+) 708 WP_218308353.1 deoxyribonuclease I -
  L6Y89_RS18110 (L6Y89_18110) rsmE 3843014..3843745 (+) 732 WP_010435451.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L6Y89_RS18115 (L6Y89_18115) gshB 3843765..3844712 (+) 948 WP_237844824.1 glutathione synthase -
  L6Y89_RS18120 (L6Y89_18120) - 3844787..3845347 (+) 561 WP_010435456.1 YqgE/AlgH family protein -
  L6Y89_RS18125 (L6Y89_18125) ruvX 3845347..3845763 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  L6Y89_RS18130 (L6Y89_18130) pilT 3845774..3846754 (-) 981 WP_237844826.1 type IV pilus twitching motility protein PilT Machinery gene
  L6Y89_RS18135 (L6Y89_18135) - 3846772..3847473 (+) 702 WP_237844828.1 YggS family pyridoxal phosphate-dependent enzyme -
  L6Y89_RS18140 (L6Y89_18140) - 3847495..3848061 (+) 567 WP_010435467.1 YggT family protein -
  L6Y89_RS18145 (L6Y89_18145) yggU 3848058..3848354 (+) 297 WP_010435470.1 DUF167 family protein YggU -
  L6Y89_RS18150 (L6Y89_18150) - 3848358..3848951 (+) 594 WP_237844830.1 XTP/dITP diphosphatase -
  L6Y89_RS18155 (L6Y89_18155) hemW 3848944..3850086 (+) 1143 WP_237844832.1 radical SAM family heme chaperone HemW -
  L6Y89_RS18160 (L6Y89_18160) - 3850150..3850512 (+) 363 WP_237844833.1 endonuclease domain-containing protein -
  L6Y89_RS18165 (L6Y89_18165) - 3850580..3851296 (-) 717 WP_042714747.1 DUF2884 domain-containing protein -
  L6Y89_RS18170 (L6Y89_18170) - 3851354..3851680 (-) 327 WP_010435485.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35664.69 Da        Isoelectric Point: 6.0399

>NTDB_id=653616 L6Y89_RS18130 WP_237844826.1 3845774..3846754(-) (pilT) [Enterobacter mori strain ACYC.E9L]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPAPDVEALLKTWLNDEQQGAWWANGQVDFAVTLTGNQRLRG
SAFTHMKGVSITLRLLPLACPQLSALGVPRAIPELLSSESGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREMGQHSLSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=653616 L6Y89_RS18130 WP_237844826.1 3845774..3846754(-) (pilT) [Enterobacter mori strain ACYC.E9L]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCGCCGCG
CTGGCGCAGATTAGGCCGCCTTGAACCCGCCCCGTTTCCGGCCCCCGACGTTGAGGCGCTATTAAAAACCTGGCTCAACG
ATGAACAGCAGGGCGCGTGGTGGGCAAACGGACAGGTAGATTTTGCCGTCACGCTCACAGGCAATCAGCGTCTTCGCGGC
AGTGCGTTTACGCATATGAAGGGGGTGTCGATCACCTTGCGACTGTTGCCGCTCGCGTGCCCGCAGCTTTCTGCACTGGG
TGTGCCCAGGGCTATCCCGGAACTGCTCTCCAGCGAAAGCGGGTTGATTCTGGTGACGGGGGCGACCGGTAGCGGAAAAT
CCACGACGCTCGCGGCGATGGTGGATTTTCTCAACCATCACACTGACGGCCATATTCTGACGCTCGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGCTGCCTGATACAGCAGCGGGAGATGGGTCAGCACAGTCTCTCCTTTGCCGAGGCGCTGCG
CAGTGCGCTGCGCGAAGATCCGGACGTTATTCTGTTGGGAGAACTGCGCGACAGCGAAACCATCCGCCTTGCGCTGACGG
CGGCCGAAACCGGGCACCTGGTGCTGGCGACGCTGCATACGCGCGGCGCTTCGCAGGCGATTGAACGGCTGGTGGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGGCAGCCTTCGCGCTGTACTGGCGCAAAAACTGCTCCA
GGACGTGCAGGGCGGGCGCGTGGCGCTTTATGAACTGCTGGTGAACACCTCGGCGGCGGCGAATCTCATCCGCGAAGGGA
AAACGTGGCAATTACCCGGCATCATACAAACCGGTCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTCGCGGAAAGG
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

100

0.512
  pilT Vibrio cholerae strain A1552

51.07

100

0.512
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377