Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LVJ84_RS10000 Genome accession   NZ_CP091516
Coordinates   1701110..1702243 (+) Length   377 a.a.
NCBI ID   WP_115308590.1    Uniprot ID   -
Organism   Kingella potus strain 3/SID/1128     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1696110..1707243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ84_RS09960 (LVJ84_09960) - 1696422..1696619 (+) 198 WP_244731459.1 hypothetical protein -
  LVJ84_RS09965 (LVJ84_09965) - 1696715..1697140 (-) 426 WP_115308596.1 MliC family protein -
  LVJ84_RS09970 (LVJ84_09970) - 1697426..1697695 (+) 270 WP_115308595.1 hypothetical protein -
  LVJ84_RS09975 (LVJ84_09975) - 1697774..1698607 (-) 834 WP_115308594.1 basic amino acid ABC transporter substrate-binding protein -
  LVJ84_RS09980 (LVJ84_09980) era 1698759..1699677 (+) 919 Protein_1992 GTPase Era -
  LVJ84_RS09985 (LVJ84_09985) - 1699857..1700030 (+) 174 WP_244731460.1 hypothetical protein -
  LVJ84_RS09990 (LVJ84_09990) - 1699979..1700554 (+) 576 WP_115308592.1 class I SAM-dependent methyltransferase -
  LVJ84_RS09995 (LVJ84_09995) - 1700575..1700946 (+) 372 WP_115308591.1 SirB2 family protein -
  LVJ84_RS10000 (LVJ84_10000) pilU 1701110..1702243 (+) 1134 WP_115308590.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LVJ84_RS10005 (LVJ84_10005) - 1702298..1702615 (+) 318 WP_115308589.1 hypothetical protein -
  LVJ84_RS10010 (LVJ84_10010) ccoS 1702617..1702829 (+) 213 WP_115308588.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  LVJ84_RS10015 (LVJ84_10015) - 1702826..1704117 (+) 1292 Protein_1999 MFS transporter -
  LVJ84_RS10020 (LVJ84_10020) - 1704236..1704601 (+) 366 WP_115308586.1 YkvA family protein -
  LVJ84_RS10030 (LVJ84_10030) miaA 1704985..1705926 (-) 942 WP_115308585.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  LVJ84_RS10035 (LVJ84_10035) - 1706214..1707035 (-) 822 WP_244731461.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41971.42 Da        Isoelectric Point: 6.1808

>NTDB_id=651965 LVJ84_RS10000 WP_115308590.1 1701110..1702243(+) (pilU) [Kingella potus strain 3/SID/1128]
MITTAEPMSVKETIHSWMRYMYENKASDLFITAEFPPAVKLDGKLTPITEKPLTAGICGAIAQALMTQKQREEFENTKEC
NFAVSIDGVARFRVNAMIQRGATALVIRIINSVIPKMDELKLPEILKKVAMQKRGLVIFVGGTGSGKSTSLAAMIDYRNE
NSYGHIITIEDPIEFVHPHKNCIITQREVGVDTDNWFAALKNTLRQAPDVILIGEIRDQETMEYALAFAETGHLCMATLH
ANSSNQALDRIINFFPEEKRTQLLTDLSLNLQAFVSQRLVPKENGKGRAAAVEVLLQSPLISDLILRGEVHGIKEIMKKS
TDIGMQTFDQALYELFESGQISYAEAIKNADSANDLRLAIQLKSRRGQNAGIDFELL

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=651965 LVJ84_RS10000 WP_115308590.1 1701110..1702243(+) (pilU) [Kingella potus strain 3/SID/1128]
ATGATAACAACCGCCGAACCGATGAGCGTCAAAGAAACCATCCACAGCTGGATGCGCTATATGTACGAAAACAAAGCGTC
CGACCTCTTCATCACAGCAGAATTCCCCCCCGCCGTGAAACTCGACGGCAAACTCACGCCGATTACCGAAAAGCCGCTCA
CCGCCGGCATCTGCGGCGCGATCGCCCAAGCCCTGATGACGCAGAAACAGCGCGAGGAATTTGAAAACACCAAAGAATGC
AACTTCGCCGTCAGCATCGACGGCGTGGCGCGTTTCCGCGTAAACGCCATGATCCAGCGCGGCGCAACGGCACTCGTCAT
CCGTATCATCAACAGCGTCATCCCGAAAATGGACGAGCTGAAACTGCCCGAAATCCTGAAAAAAGTCGCCATGCAGAAAC
GCGGGCTGGTCATTTTTGTCGGCGGCACCGGTTCGGGCAAATCCACCTCGCTGGCCGCCATGATCGACTACCGCAACGAA
AACAGCTACGGCCACATCATCACCATCGAAGACCCCATCGAATTTGTCCATCCCCACAAAAACTGCATCATCACCCAGCG
CGAAGTCGGCGTGGACACCGACAACTGGTTTGCCGCCCTGAAAAACACCCTGCGCCAGGCTCCCGATGTCATCCTGATCG
GCGAAATCCGCGACCAGGAAACCATGGAATACGCACTGGCCTTCGCCGAAACCGGCCACCTGTGCATGGCCACCCTCCAC
GCCAACAGCTCCAACCAGGCACTCGACCGCATCATCAACTTCTTCCCCGAAGAAAAACGCACCCAGCTTCTGACCGACCT
CTCCCTCAACCTCCAAGCCTTCGTTTCCCAACGCCTCGTGCCCAAAGAAAACGGCAAAGGCCGTGCGGCGGCGGTGGAAG
TGCTGTTGCAGTCGCCGCTGATTTCCGACCTGATTTTGCGCGGCGAAGTGCACGGCATCAAAGAAATCATGAAAAAATCC
ACCGACATCGGTATGCAGACCTTCGACCAGGCCCTGTACGAACTCTTTGAAAGCGGCCAGATTTCCTACGCCGAAGCCAT
CAAAAACGCCGACTCGGCCAACGACCTGCGCCTGGCCATCCAGCTCAAAAGCCGGCGCGGCCAAAACGCTGGCATCGACT
TCGAGCTTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-281)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.519

92.042

0.557
  pilU Vibrio cholerae strain A1552

54.595

98.143

0.536
  pilU Acinetobacter baylyi ADP1

57.637

92.042

0.531
  pilT Legionella pneumophila strain ERS1305867

44.681

87.268

0.39
  pilT Legionella pneumophila strain Lp02

44.681

87.268

0.39
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.027

89.39

0.385
  pilT Pseudomonas aeruginosa PAK

42.73

89.39

0.382
  pilT Acinetobacter nosocomialis M2

43.161

87.268

0.377
  pilT Acinetobacter baumannii D1279779

43.161

87.268

0.377
  pilT Acinetobacter baumannii strain A118

43.161

87.268

0.377
  pilT Pseudomonas stutzeri DSM 10701

41.543

89.39

0.371
  pilT Acinetobacter baylyi ADP1

42.042

88.329

0.371
  pilT Neisseria meningitidis 8013

39.826

91.247

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.265

88.064

0.363
  pilT Vibrio cholerae strain A1552

41.265

88.064

0.363