Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LVJ83_RS07315 Genome accession   NZ_CP091508
Coordinates   1591833..1592960 (-) Length   375 a.a.
NCBI ID   WP_244783871.1    Uniprot ID   -
Organism   Uruburuella testudinis strain CCUG 63373m     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1586833..1597960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ83_RS07285 (LVJ83_07285) - 1587747..1588511 (-) 765 WP_244783865.1 ATP-binding cassette domain-containing protein -
  LVJ83_RS07290 (LVJ83_07290) - 1588630..1589175 (-) 546 WP_244783866.1 DUF4157 domain-containing protein -
  LVJ83_RS07295 (LVJ83_07295) - 1589168..1589704 (-) 537 WP_244783867.1 DUF2062 domain-containing protein -
  LVJ83_RS07300 (LVJ83_07300) - 1589858..1591183 (-) 1326 WP_244783868.1 MFS transporter -
  LVJ83_RS07305 (LVJ83_07305) ccoS 1591167..1591370 (-) 204 WP_244783869.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  LVJ83_RS07310 (LVJ83_07310) - 1591372..1591689 (-) 318 WP_244783870.1 hypothetical protein -
  LVJ83_RS07315 (LVJ83_07315) pilU 1591833..1592960 (-) 1128 WP_244783871.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LVJ83_RS07320 (LVJ83_07320) - 1593166..1593885 (+) 720 WP_244783872.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  LVJ83_RS07325 (LVJ83_07325) - 1594281..1594541 (-) 261 WP_244783873.1 GNAT family N-acetyltransferase -
  LVJ83_RS07330 (LVJ83_07330) - 1594933..1595796 (+) 864 WP_244783874.1 formate/nitrite transporter family protein -
  LVJ83_RS07335 (LVJ83_07335) - 1596072..1597385 (-) 1314 WP_244783875.1 anaerobic C4-dicarboxylate transporter -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42113.17 Da        Isoelectric Point: 6.5645

>NTDB_id=651790 LVJ83_RS07315 WP_244783871.1 1591833..1592960(-) (pilU) [Uruburuella testudinis strain CCUG 63373m]
MEHNPPSAKDALYTWLRHLNKYKGSDLFITTNFPPAMKVDGKITPISDEPLSAERCMEIAFSIMSSKQIEEFTATNECNF
AISLPDTSRFRVNAMVQRGASALVLRAITSEIPKFENLNLPPVLQKIALKKRGLVIFVGGTGSGKSTSLASMIDYRNEHS
FDHIITIEDPIEFIHQHKNCIISQREVGVDTDNWHIALKNTLRQAPDVILIGEIRDRETMDYAISFAETGHLCMATLHAN
STNQALDRIINFFPEERRTQLLTDLSLNLQAFVSQRLIPREHGKGRVAAVEVMLNSPLIAELIHKGEVHAIKEVMKKSQA
AGMQTFDQALYDLYENSHISLQEALKNADSANDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=651790 LVJ83_RS07315 WP_244783871.1 1591833..1592960(-) (pilU) [Uruburuella testudinis strain CCUG 63373m]
ATGGAACACAACCCACCATCCGCCAAAGATGCGCTCTACACCTGGCTGCGCCATCTCAATAAATACAAAGGCTCTGATCT
CTTCATCACCACCAACTTTCCGCCGGCGATGAAAGTAGACGGTAAAATCACCCCCATCAGCGATGAGCCGCTCAGCGCCG
AGCGTTGCATGGAAATTGCTTTTTCGATTATGAGCAGCAAACAAATCGAAGAATTCACCGCCACCAACGAATGCAACTTC
GCCATCAGCCTGCCCGACACCAGCCGCTTCCGTGTTAATGCCATGGTGCAGCGCGGCGCATCGGCCTTGGTGTTGCGCGC
CATTACCAGCGAAATTCCCAAATTTGAAAACCTCAACCTGCCCCCGGTTTTACAGAAAATCGCCTTAAAAAAACGCGGTC
TGGTGATTTTTGTGGGCGGCACCGGCTCTGGAAAATCCACCTCGCTGGCTTCAATGATTGACTACCGCAATGAACACAGC
TTCGACCATATCATCACCATCGAAGACCCGATCGAATTTATCCACCAACACAAAAACTGCATCATCAGCCAGCGCGAAGT
GGGCGTTGATACCGACAACTGGCACATCGCCCTGAAAAACACCTTGCGCCAGGCGCCCGATGTGATTCTGATCGGCGAAA
TCCGTGACCGCGAAACCATGGATTACGCCATCTCATTTGCCGAAACCGGCCACCTCTGCATGGCCACGCTGCATGCCAAC
AGCACCAATCAGGCGCTCGACCGCATCATCAACTTTTTTCCCGAAGAACGCCGCACCCAGCTGCTCACCGATCTCTCGCT
CAATTTGCAGGCTTTTGTATCGCAACGGCTGATTCCGCGCGAACACGGCAAAGGGCGCGTGGCTGCTGTAGAGGTGATGC
TCAATTCACCGCTGATTGCCGAGCTGATTCACAAAGGCGAAGTGCATGCCATCAAAGAAGTGATGAAAAAATCACAGGCA
GCCGGCATGCAGACTTTCGACCAGGCCTTATATGATTTGTATGAAAACAGCCACATCAGCCTGCAAGAAGCGCTTAAAAA
TGCCGATTCCGCCAACGATTTGCGTCTCGACATCCAATTGCGCAGCCGCCGCGCCCAAAACGCCGGCCCCGATTTAGAAT
TAATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(39-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.96

92.533

0.573
  pilU Acinetobacter baylyi ADP1

58.309

91.467

0.533
  pilU Vibrio cholerae strain A1552

53.631

95.467

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.844

88.8

0.389
  pilT Legionella pneumophila strain Lp02

43.827

86.4

0.379
  pilT Legionella pneumophila strain ERS1305867

43.827

86.4

0.379
  pilT Acinetobacter baylyi ADP1

41.768

87.467

0.365
  pilT Pseudomonas stutzeri DSM 10701

41.641

87.733

0.365
  pilT Pseudomonas aeruginosa PAK

41.641

87.733

0.365
  pilT Neisseria meningitidis 8013

40.597

89.333

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.975

86.4

0.363
  pilT Vibrio cholerae strain A1552

41.975

86.4

0.363
  pilT Acinetobacter baumannii strain A118

41.667

86.4

0.36
  pilT Acinetobacter baumannii D1279779

41.667

86.4

0.36
  pilT Acinetobacter nosocomialis M2

41.667

86.4

0.36