Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L3071_RS20165 Genome accession   NZ_CP091496
Coordinates   4246941..4247921 (+) Length   326 a.a.
NCBI ID   WP_032676501.1    Uniprot ID   A0A9Q2WBJ7
Organism   Enterobacter cloacae complex sp. ECL404     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4241941..4252921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3071_RS20125 (L3071_20125) - 4242024..4242350 (+) 327 WP_003862421.1 YggL family protein -
  L3071_RS20130 (L3071_20130) - 4242408..4243124 (+) 717 WP_022649360.1 DUF2884 domain-containing protein -
  L3071_RS20135 (L3071_20135) - 4243208..4243546 (-) 339 WP_023298571.1 endonuclease domain-containing protein -
  L3071_RS20140 (L3071_20140) hemW 4243603..4244751 (-) 1149 WP_038416710.1 radical SAM family heme chaperone HemW -
  L3071_RS20145 (L3071_20145) - 4244744..4245337 (-) 594 WP_022649357.1 XTP/dITP diphosphatase -
  L3071_RS20150 (L3071_20150) yggU 4245341..4245637 (-) 297 WP_022649356.1 DUF167 family protein YggU -
  L3071_RS20155 (L3071_20155) - 4245634..4246200 (-) 567 WP_003860024.1 YggT family protein -
  L3071_RS20160 (L3071_20160) - 4246222..4246923 (-) 702 WP_023298569.1 YggS family pyridoxal phosphate-dependent enzyme -
  L3071_RS20165 (L3071_20165) pilT 4246941..4247921 (+) 981 WP_032676501.1 type IV pilus twitching motility protein PilT Machinery gene
  L3071_RS20170 (L3071_20170) ruvX 4247932..4248348 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  L3071_RS20175 (L3071_20175) - 4248348..4248908 (-) 561 WP_022649353.1 YqgE/AlgH family protein -
  L3071_RS20180 (L3071_20180) gshB 4248983..4249930 (-) 948 WP_038416709.1 glutathione synthase -
  L3071_RS20185 (L3071_20185) rsmE 4249962..4250693 (-) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L3071_RS20190 (L3071_20190) endA 4250745..4251452 (-) 708 WP_038416708.1 deoxyribonuclease I -
  L3071_RS20195 (L3071_20195) - 4251547..4252044 (-) 498 WP_032621896.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35673.66 Da        Isoelectric Point: 6.6603

>NTDB_id=651725 L3071_RS20165 WP_032676501.1 4246941..4247921(+) (pilT) [Enterobacter cloacae complex sp. ECL404]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSQRCLIRQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=651725 L3071_RS20165 WP_032676501.1 4246941..4247921(+) (pilT) [Enterobacter cloacae complex sp. ECL404]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAGCAGCAGGGTGCCTGGTGGGCAAACGGGCAGGTAGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTACGCCTGTTGCCGCTGACGTGTCCGCAGCTCTCTGCGCTAGG
CGTGCCGCGAGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTTCTCAATCACCATACGGACGGGCACATTCTGACTCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTCAACGTTGCCTCATCCGGCAGCGGGAGATAGGCCAGCATAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGTGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCTGAGACTGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTTCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTACTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38