Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L3068_RS18980 Genome accession   NZ_CP091481
Coordinates   3957588..3958568 (-) Length   326 a.a.
NCBI ID   WP_236904762.1    Uniprot ID   -
Organism   Enterobacter cloacae complex sp. ECL414     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3952588..3963568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3068_RS18950 (L3068_18950) - 3953477..3953974 (+) 498 WP_117582191.1 SprT family zinc-dependent metalloprotease -
  L3068_RS18955 (L3068_18955) endA 3954069..3954776 (+) 708 WP_014885187.1 deoxyribonuclease I -
  L3068_RS18960 (L3068_18960) rsmE 3954828..3955559 (+) 732 WP_023331277.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L3068_RS18965 (L3068_18965) gshB 3955579..3956526 (+) 948 WP_023331278.1 glutathione synthase -
  L3068_RS18970 (L3068_18970) - 3956601..3957161 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  L3068_RS18975 (L3068_18975) ruvX 3957161..3957577 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  L3068_RS18980 (L3068_18980) pilT 3957588..3958568 (-) 981 WP_236904762.1 type IV pilus twitching motility protein PilT Machinery gene
  L3068_RS18985 (L3068_18985) - 3958586..3959287 (+) 702 WP_014885191.1 YggS family pyridoxal phosphate-dependent enzyme -
  L3068_RS18990 (L3068_18990) - 3959309..3959875 (+) 567 WP_014885192.1 YggT family protein -
  L3068_RS18995 (L3068_18995) yggU 3959872..3960168 (+) 297 WP_045909934.1 DUF167 family protein YggU -
  L3068_RS19000 (L3068_19000) - 3960172..3960765 (+) 594 WP_023331280.1 XTP/dITP diphosphatase -
  L3068_RS19005 (L3068_19005) hemW 3960758..3961900 (+) 1143 WP_014885195.1 radical SAM family heme chaperone HemW -
  L3068_RS19010 (L3068_19010) - 3961964..3962302 (+) 339 WP_117582193.1 endonuclease domain-containing protein -
  L3068_RS19015 (L3068_19015) - 3962384..3963100 (-) 717 WP_014885197.1 DUF2884 domain-containing protein -
  L3068_RS19020 (L3068_19020) - 3963157..3963483 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35580.53 Da        Isoelectric Point: 6.4113

>NTDB_id=651661 L3068_RS18980 WP_236904762.1 3957588..3958568(-) (pilT) [Enterobacter cloacae complex sp. ECL414]
MDVEEMVALSVKHNVSDLHLCSDSPSRWRRSGRLEPAPFPPPDVGALLKAWLNDEQQGSWWAHGQVDFAVTVAEGQRLRG
SAFKQINGVSIALRLLPRTCSPLSSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
YLYRSERCLIQQREIGQHCPSFVEALRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSVAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=651661 L3068_RS18980 WP_236904762.1 3957588..3958568(-) (pilT) [Enterobacter cloacae complex sp. ECL414]
ATGGATGTGGAAGAAATGGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCATCCCG
CTGGCGCCGGTCAGGACGCCTTGAACCCGCGCCGTTTCCGCCGCCAGATGTCGGGGCGTTATTAAAAGCGTGGCTCAATG
ATGAGCAGCAGGGAAGCTGGTGGGCTCACGGTCAGGTCGATTTTGCCGTGACGGTGGCAGAAGGCCAGCGTCTGCGCGGG
AGTGCGTTTAAGCAGATTAACGGGGTGTCCATCGCCCTGCGGTTGCTGCCACGCACCTGTTCGCCACTCTCTTCGCTGGG
CGTGCCGCGTGCCATTCCGGAACTGTTATCCAGTGACAACGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACCACTCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGGCATATCCTCACGCTTGAAGATCCGGTGGAG
TATCTGTACCGGAGCGAACGCTGCCTGATTCAGCAGCGGGAGATAGGTCAACACTGCCCGTCTTTTGTCGAGGCGCTGCG
CGGAGCGTTACGCGAGGATCCAGATGTGATTTTGCTGGGGGAGCTGCGTGATGCAGAGACGATCCGTCTCGCGTTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACACTGCATACGCGGGGTGCCTCGCAGGCCATTGAGCGGCTGGTGGACACC
TTCCCGGCGCAGGAGAAAGACCCGGTACGTAACCAGCTGGCCGGTAGCCTGCGTGCGGTGCTGGCACAGAGGCTGCTTCC
CGACCTGCAGGGTGGGCGTGTCGCGCTGTATGAACTGCTGGTGAATACCCCGGCGGCGGCAAATCTGATTCGTGAAGGAA
AAACGTGGCAACTGCCCGGTATCATTCAAACAGGCCAGCAGGCGGGAATGCAGAACTTTGATCAAAGCGTGGCGGAGAGA
CGGGCGCAGGGGCGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

45.748

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Legionella pneumophila strain ERS1305867

48.137

98.773

0.475
  pilT Legionella pneumophila strain Lp02

48.137

98.773

0.475
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.547

98.773

0.42
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38