Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LU631_RS20575 Genome accession   NZ_CP089940
Coordinates   3924705..3925700 (-) Length   331 a.a.
NCBI ID   WP_016190291.1    Uniprot ID   A0A0M2KDW2
Organism   Erwinia tracheiphila strain BuffGH     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3919705..3930700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LU631_RS20545 (LU631_20545) - 3920437..3920961 (+) 525 WP_040465323.1 SprT family zinc-dependent metalloprotease -
  LU631_RS20550 (LU631_20550) endA 3921067..3921780 (+) 714 WP_016190286.1 deoxyribonuclease I -
  LU631_RS20555 (LU631_20555) rsmE 3921855..3922586 (+) 732 WP_016190287.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LU631_RS20560 (LU631_20560) gshB 3922670..3923614 (+) 945 WP_016190288.1 glutathione synthase -
  LU631_RS20565 (LU631_20565) - 3923708..3924271 (+) 564 WP_016190289.1 YqgE/AlgH family protein -
  LU631_RS20570 (LU631_20570) ruvX 3924271..3924693 (+) 423 WP_016190290.1 Holliday junction resolvase RuvX -
  LU631_RS20575 (LU631_20575) pilT 3924705..3925700 (-) 996 WP_016190291.1 type IV pilus twitching motility protein PilT Machinery gene
  LU631_RS20580 (LU631_20580) - 3925715..3926422 (+) 708 WP_016190292.1 YggS family pyridoxal phosphate-dependent enzyme -
  LU631_RS20585 (LU631_20585) - 3926431..3926982 (+) 552 WP_016190293.1 YggT family protein -
  LU631_RS20590 (LU631_20590) - 3927013..3927609 (+) 597 WP_016190294.1 XTP/dITP diphosphatase -
  LU631_RS20595 (LU631_20595) hemW 3927602..3928762 (+) 1161 WP_016190295.1 radical SAM family heme chaperone HemW -
  LU631_RS20600 (LU631_20600) - 3928909..3929948 (+) 1040 Protein_4058 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 35924.11 Da        Isoelectric Point: 7.2438

>NTDB_id=639281 LU631_RS20575 WP_016190291.1 3924705..3925700(-) (pilT) [Erwinia tracheiphila strain BuffGH]
MHIEDIVALSVKHKAGDLHLCSGYPPFWRRDGRLQKIADAPPVSNEVLHAAAQEWLDGAQQVHFEQAGQIDFALTFSDGV
RVRANLFRQRQGLSLALRIINGHISGTEQLELPQVTRDLLQLHDGLVLVTGATGSGKSTTLAALTGEINQHQARHILTLE
DPIEFIHHSARSLVQQREIGTHCVSFSEGLRAALREDPDVILLGELRDKGTIQLALTAAETGHLVLATLHTRGAVQAVDR
LVDVFPGAEKDLIRTQLAGSLKVVIAQKLVPAVKSGRVALFEVLVSTTAVANLIREGKTHQLPAVLETGVAHGMQSFRQS
LAARRQAGQVE

Nucleotide


Download         Length: 996 bp        

>NTDB_id=639281 LU631_RS20575 WP_016190291.1 3924705..3925700(-) (pilT) [Erwinia tracheiphila strain BuffGH]
ATGCATATCGAGGACATAGTGGCCCTTAGTGTAAAGCATAAGGCAGGGGATCTGCACCTGTGTAGTGGTTATCCGCCATT
CTGGCGGCGTGATGGACGGTTGCAAAAAATAGCCGATGCGCCCCCGGTCAGCAATGAGGTGTTACACGCTGCGGCACAGG
AATGGCTCGACGGTGCGCAACAGGTGCATTTTGAGCAGGCCGGACAGATTGATTTTGCGCTGACATTTTCAGATGGCGTG
CGTGTACGTGCAAACCTCTTTCGCCAGCGGCAGGGCCTTTCGCTGGCGCTGCGCATTATTAACGGCCATATTTCTGGTAC
AGAGCAGCTTGAGCTACCCCAGGTCACCCGGGATCTGCTCCAGCTGCATGATGGTCTGGTGCTGGTGACAGGCGCAACCG
GTAGCGGTAAATCCACAACGTTGGCAGCGCTGACCGGCGAAATTAACCAACACCAGGCGCGGCATATTCTGACGTTGGAA
GATCCCATTGAATTTATCCACCATAGTGCGCGCTCGCTGGTTCAGCAGCGTGAAATCGGCACGCACTGTGTCTCATTCAG
TGAGGGGCTGCGGGCGGCATTGCGTGAAGATCCGGATGTCATCCTGCTGGGAGAACTGCGCGACAAAGGGACTATTCAGC
TGGCGCTGACTGCGGCAGAAACCGGGCATCTTGTGCTGGCTACCCTGCATACCCGTGGAGCCGTGCAGGCGGTGGACAGG
CTGGTCGACGTGTTTCCCGGCGCGGAAAAAGACCTGATACGTACGCAGCTGGCAGGAAGCCTGAAGGTGGTGATTGCTCA
GAAGCTGGTTCCGGCAGTGAAGTCGGGCAGGGTTGCGCTGTTTGAGGTGCTGGTGAGTACGACCGCCGTGGCCAATCTGA
TCCGTGAAGGTAAAACGCACCAGCTGCCCGCTGTGCTGGAAACCGGCGTAGCGCACGGGATGCAATCTTTCAGGCAAAGC
CTTGCGGCAAGACGGCAGGCAGGGCAGGTAGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(66-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M2KDW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

46.221

100

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.438

96.677

0.468
  pilT Vibrio cholerae strain A1552

48.438

96.677

0.468
  pilT Neisseria meningitidis 8013

47.826

97.281

0.465
  pilT Legionella pneumophila strain Lp02

46.417

96.979

0.45
  pilT Pseudomonas stutzeri DSM 10701

46.417

96.979

0.45
  pilT Legionella pneumophila strain ERS1305867

46.417

96.979

0.45
  pilT Acinetobacter baylyi ADP1

46.106

96.979

0.447
  pilT Pseudomonas aeruginosa PAK

45.794

96.979

0.444
  pilT Acinetobacter baumannii D1279779

45.483

96.979

0.441
  pilT Acinetobacter baumannii strain A118

45.483

96.979

0.441
  pilT Acinetobacter nosocomialis M2

45.483

96.979

0.441
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.865

98.489

0.432
  pilU Vibrio cholerae strain A1552

42.949

94.26

0.405
  pilU Pseudomonas stutzeri DSM 10701

38.323

100

0.387