Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LVE68_RS11845 Genome accession   NZ_CP089928
Coordinates   2748387..2749535 (-) Length   382 a.a.
NCBI ID   WP_232940612.1    Uniprot ID   -
Organism   Microbulbifer sp. YPW16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2743387..2754535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVE68_RS11830 (LVE68_11830) - 2743424..2744557 (+) 1134 WP_232940609.1 sensor histidine kinase -
  LVE68_RS11835 (LVE68_11835) - 2744554..2745303 (+) 750 WP_232940610.1 LytTR family DNA-binding domain-containing protein -
  LVE68_RS11840 (LVE68_11840) - 2745464..2748154 (-) 2691 WP_232940611.1 kelch repeat-containing protein -
  LVE68_RS11845 (LVE68_11845) pilU 2748387..2749535 (-) 1149 WP_232940612.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LVE68_RS11850 (LVE68_11850) pilT 2749628..2750662 (-) 1035 WP_232940613.1 type IV pilus twitching motility protein PilT Machinery gene
  LVE68_RS11855 (LVE68_11855) - 2750788..2751486 (+) 699 WP_232940614.1 YggS family pyridoxal phosphate-dependent enzyme -
  LVE68_RS11860 (LVE68_11860) proC 2751590..2752417 (+) 828 WP_334078283.1 pyrroline-5-carboxylate reductase -
  LVE68_RS11865 (LVE68_11865) - 2752469..2753074 (+) 606 WP_232940615.1 YggT family protein -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42607.93 Da        Isoelectric Point: 6.2409

>NTDB_id=639181 LVE68_RS11845 WP_232940612.1 2748387..2749535(-) (pilU) [Microbulbifer sp. YPW16]
MEIERLLQLVVEKGASDLFITAGVPPSIKVHGKVVPASSTALTPEKAREMVVGVMNEKQRREFAEKKELNFAIAVRNVGR
FRVSAFFQRNLVGMVLRRIETKIPTIDGLGLPEVVKELAMTKRGLIIFVGATGTGKSTSLASMIGHRNENSKGHIISIED
PIEFVHQHRGCIVTQREVGLDTESFEVALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHQQLWMDLSLNLRAMVAQQLVPTPDGDGRRACLEIMINTPLASDLIRKGEVHKLKELMKRSNEQGMQTFDQA
LYELYDRGEITYEDALAHADSANDLRLMIKLKSESDAEYLNEAAGELSLEGETDDYKGPQIF

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=639181 LVE68_RS11845 WP_232940612.1 2748387..2749535(-) (pilU) [Microbulbifer sp. YPW16]
ATGGAAATCGAAAGACTCCTCCAGCTTGTCGTCGAGAAAGGCGCATCCGACCTGTTTATCACTGCCGGGGTGCCGCCCTC
AATCAAGGTGCACGGCAAGGTGGTGCCCGCCAGCAGCACGGCTCTGACCCCGGAAAAGGCCCGCGAGATGGTCGTCGGGG
TAATGAACGAGAAACAGCGGCGCGAGTTCGCGGAAAAGAAAGAGCTCAACTTTGCCATCGCGGTGCGCAATGTGGGCCGC
TTCCGGGTCTCGGCGTTCTTCCAGCGCAACCTGGTGGGCATGGTGCTGCGGCGGATCGAGACCAAGATCCCCACCATCGA
TGGCCTGGGGCTGCCGGAAGTGGTCAAGGAACTGGCGATGACCAAGCGCGGCCTGATCATCTTCGTGGGTGCCACCGGTA
CCGGTAAGTCCACGTCACTGGCCTCGATGATCGGCCACCGCAATGAGAACTCCAAGGGACACATCATCTCGATCGAGGAC
CCGATCGAATTCGTCCACCAGCACCGCGGCTGTATCGTGACCCAGCGCGAGGTGGGCCTGGATACCGAATCCTTCGAAGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATTGGTGAGGTGCGCTCCCGCGAAACCATGGACCACG
CCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGTGTACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGGATC
ATCCACTTCTTCCCCGCCGACCGGCACCAGCAACTGTGGATGGACCTGTCCCTGAACCTGAGGGCCATGGTGGCCCAGCA
GCTGGTACCCACCCCGGATGGCGACGGCCGCCGCGCGTGCCTGGAAATCATGATCAACACCCCGCTGGCCTCGGACCTGA
TCCGCAAGGGCGAGGTGCACAAGCTGAAAGAGTTGATGAAGCGCTCCAACGAGCAGGGGATGCAGACTTTCGACCAGGCC
CTGTACGAACTGTACGACCGCGGCGAGATCACCTACGAGGACGCCCTCGCCCACGCCGACTCCGCCAACGACCTGCGCCT
GATGATCAAGCTCAAGTCAGAATCCGACGCCGAGTACCTGAACGAGGCAGCGGGCGAGCTGAGCCTTGAAGGCGAGACTG
ACGACTACAAGGGACCGCAGATTTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

76.533

98.168

0.751
  pilU Acinetobacter baylyi ADP1

62.924

100

0.631
  pilU Vibrio cholerae strain A1552

57.022

93.194

0.531
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.133

88.743

0.401
  pilT Acinetobacter baumannii D1279779

43.284

87.696

0.38
  pilT Acinetobacter nosocomialis M2

43.284

87.696

0.38
  pilT Acinetobacter baumannii strain A118

43.284

87.696

0.38
  pilT Vibrio cholerae strain A1552

42.515

87.435

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.515

87.435

0.372
  pilT Legionella pneumophila strain ERS1305867

42.09

87.696

0.369
  pilT Legionella pneumophila strain Lp02

42.09

87.696

0.369
  pilT Acinetobacter baylyi ADP1

41.056

89.267

0.366
  pilT Pseudomonas aeruginosa PAK

41.791

87.696

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.194

87.696

0.361