Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   LVB77_RS21310 Genome accession   NZ_CP089924
Coordinates   4043235..4043651 (+) Length   138 a.a.
NCBI ID   WP_305068907.1    Uniprot ID   -
Organism   Lysobacter sp. 5GHs7-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4038235..4048651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVB77_RS18180 (LVB77_18180) - 4038490..4039089 (-) 600 WP_232907474.1 class I SAM-dependent methyltransferase -
  LVB77_RS18185 (LVB77_18185) - 4039091..4040122 (-) 1032 WP_232907475.1 glycosyltransferase -
  LVB77_RS18190 (LVB77_18190) - 4040119..4040913 (-) 795 WP_232907476.1 glycosyltransferase family A protein -
  LVB77_RS18195 (LVB77_18195) - 4040925..4041287 (-) 363 WP_232907478.1 EamA family transporter -
  LVB77_RS18200 (LVB77_18200) pilA 4041761..4042180 (+) 420 WP_232907479.1 pilin Machinery gene
  LVB77_RS18205 (LVB77_18205) pilA2 4042537..4042956 (+) 420 WP_232907480.1 pilin Machinery gene
  LVB77_RS21310 pilA2 4043235..4043651 (+) 417 WP_305068907.1 pilin Machinery gene
  LVB77_RS18220 (LVB77_18220) - 4043777..4045720 (+) 1944 WP_232907482.1 hypothetical protein -
  LVB77_RS21315 - 4045961..4046389 (+) 429 WP_305068908.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LVB77_RS18235 (LVB77_18235) - 4046579..4048540 (+) 1962 WP_232907483.1 hypothetical protein -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14440.47 Da        Isoelectric Point: 6.8958

>NTDB_id=639120 LVB77_RS21310 WP_305068907.1 4043235..4043651(+) (pilA2) [Lysobacter sp. 5GHs7-4]
MNKQQGFTLIELMIVIAILGILIAIALPAYQDYTIRTKNTECLNVAAAAKLAVSETAQDRGSLNLVTSTNTGYSFTASDY
CNRVAVGANGVIGMTTRSTGAAANAVHTLTPAMPAGSGRIEWTCTSNAPDSQIPAECR

Nucleotide


Download         Length: 417 bp        

>NTDB_id=639120 LVB77_RS21310 WP_305068907.1 4043235..4043651(+) (pilA2) [Lysobacter sp. 5GHs7-4]
ATGAACAAGCAGCAAGGCTTCACTCTGATCGAACTGATGATCGTCATCGCGATCCTGGGCATCCTGATCGCCATCGCCCT
GCCGGCGTACCAGGACTACACCATCCGCACGAAGAACACCGAGTGCCTGAACGTCGCCGCGGCCGCCAAGCTGGCCGTGT
CGGAGACCGCCCAGGACCGCGGTTCGCTGAACCTGGTCACCTCGACCAACACCGGCTACTCCTTCACCGCCAGCGACTAC
TGCAACCGCGTCGCGGTCGGCGCCAACGGCGTGATCGGCATGACCACCCGCAGCACCGGCGCCGCCGCCAACGCCGTGCA
CACCCTGACCCCGGCCATGCCGGCCGGTTCGGGCCGCATCGAGTGGACCTGCACCTCCAACGCTCCGGACTCGCAGATTC
CGGCCGAGTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

51.449

100

0.514
  pilA2 Legionella pneumophila str. Paris

51.449

100

0.514
  pilA Ralstonia pseudosolanacearum GMI1000

41.566

100

0.5
  comP Acinetobacter baylyi ADP1

42.568

100

0.457
  pilA/pilA1 Eikenella corrodens VA1

39.49

100

0.449
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.365

100

0.442
  pilE Neisseria gonorrhoeae strain FA1090

36.538

100

0.413
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.876

99.275

0.406
  pilA Haemophilus influenzae 86-028NP

37.857

100

0.384
  pilA Pseudomonas aeruginosa PAK

35.099

100

0.384
  pilE Neisseria gonorrhoeae MS11

40.945

92.029

0.377
  pilA Haemophilus influenzae Rd KW20

36.957

100

0.37
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.037

97.826

0.362