Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LVB77_RS18200 Genome accession   NZ_CP089924
Coordinates   4041761..4042180 (+) Length   139 a.a.
NCBI ID   WP_232907479.1    Uniprot ID   -
Organism   Lysobacter sp. 5GHs7-4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4036761..4047180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVB77_RS18175 (LVB77_18175) pilR 4037034..4038449 (+) 1416 WP_232907473.1 sigma-54 dependent transcriptional regulator Regulator
  LVB77_RS18180 (LVB77_18180) - 4038490..4039089 (-) 600 WP_232907474.1 class I SAM-dependent methyltransferase -
  LVB77_RS18185 (LVB77_18185) - 4039091..4040122 (-) 1032 WP_232907475.1 glycosyltransferase -
  LVB77_RS18190 (LVB77_18190) - 4040119..4040913 (-) 795 WP_232907476.1 glycosyltransferase family A protein -
  LVB77_RS18195 (LVB77_18195) - 4040925..4041287 (-) 363 WP_232907478.1 EamA family transporter -
  LVB77_RS18200 (LVB77_18200) pilA 4041761..4042180 (+) 420 WP_232907479.1 pilin Machinery gene
  LVB77_RS18205 (LVB77_18205) pilA2 4042537..4042956 (+) 420 WP_232907480.1 pilin Machinery gene
  LVB77_RS21310 pilA2 4043235..4043651 (+) 417 WP_305068907.1 pilin Machinery gene
  LVB77_RS18220 (LVB77_18220) - 4043777..4045720 (+) 1944 WP_232907482.1 hypothetical protein -
  LVB77_RS21315 - 4045961..4046389 (+) 429 WP_305068908.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14725.77 Da        Isoelectric Point: 5.0909

>NTDB_id=639114 LVB77_RS18200 WP_232907479.1 4041761..4042180(+) (pilA) [Lysobacter sp. 5GHs7-4]
MKNNQQGFTLIELMIVIAILGILIAIALPAYQDYTIRTKNTECLNVAAAAKLAVSETAQDRGSLNLVTDTNTGYSFTASD
YCREVKVNANGVIGMVTKSTGAAADAAHTLTPTMPAGSGRIEWKCESNAPDSQVPAECR

Nucleotide


Download         Length: 420 bp        

>NTDB_id=639114 LVB77_RS18200 WP_232907479.1 4041761..4042180(+) (pilA) [Lysobacter sp. 5GHs7-4]
ATGAAAAACAATCAGCAAGGCTTCACCCTGATCGAACTGATGATCGTCATCGCGATCCTGGGCATCCTGATCGCCATCGC
CCTGCCGGCCTACCAGGACTACACCATCCGCACCAAGAACACCGAGTGCCTGAACGTCGCCGCGGCCGCCAAGCTGGCCG
TGTCCGAGACCGCCCAGGATCGTGGCTCGCTGAACCTGGTCACCGACACCAACACCGGCTACTCCTTCACCGCCAGCGAC
TACTGCAGAGAGGTCAAGGTCAACGCCAACGGTGTGATCGGGATGGTAACCAAGAGCACCGGTGCCGCCGCCGACGCTGC
TCACACCCTGACCCCCACCATGCCGGCCGGCTCGGGCCGCATCGAGTGGAAGTGCGAGTCGAACGCCCCGGACTCGCAGG
TCCCGGCCGAGTGCCGCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-28)

Pilin (bacterial filament) (Pilin)

(37-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

41.212

100

0.489
  pilA2 Legionella pneumophila strain ERS1305867

48.905

98.561

0.482
  pilA2 Legionella pneumophila str. Paris

48.905

98.561

0.482
  comP Acinetobacter baylyi ADP1

43.537

100

0.46
  pilA/pilA1 Eikenella corrodens VA1

38.065

100

0.424
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.146

98.561

0.396
  pilE Neisseria gonorrhoeae MS11

40

93.525

0.374
  pilE Neisseria gonorrhoeae strain FA1090

40.31

92.806

0.374
  pilA Pseudomonas aeruginosa PAK

35.135

100

0.374