Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U719_RS11590 Genome accession   NC_022794
Coordinates   2180739..2181785 (-) Length   348 a.a.
NCBI ID   WP_023468959.1    Uniprot ID   A0A0V8GJH8
Organism   Exiguobacterium sp. MH3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2175739..2186785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U719_RS11570 (U719_11825) - 2176330..2178048 (-) 1719 WP_023468955.1 fimbrial assembly protein -
  U719_RS11575 (U719_11830) - 2178067..2178822 (-) 756 WP_023468956.1 A24 family peptidase -
  U719_RS11580 (U719_11835) - 2178885..2179379 (-) 495 WP_023468957.1 type II secretion system protein -
  U719_RS11585 (U719_11840) - 2179517..2180734 (-) 1218 WP_023468958.1 type II secretion system F family protein -
  U719_RS11590 (U719_11845) pilT 2180739..2181785 (-) 1047 WP_023468959.1 type IV pilus twitching motility protein PilT Machinery gene
  U719_RS11595 (U719_11850) - 2181782..2183446 (-) 1665 WP_023468960.1 GspE/PulE family protein -
  U719_RS11600 (U719_11855) - 2183455..2183769 (-) 315 WP_023468961.1 hypothetical protein -
  U719_RS11605 (U719_11860) - 2183786..2185120 (-) 1335 WP_041255164.1 G5 domain-containing protein -
  U719_RS11610 (U719_11865) - 2185297..2185824 (+) 528 WP_023468963.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  U719_RS11615 (U719_11870) - 2185787..2186407 (+) 621 WP_023468964.1 PilW family protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38232.94 Da        Isoelectric Point: 8.0678

>NTDB_id=63500 U719_RS11590 WP_023468959.1 2180739..2181785(-) (pilT) [Exiguobacterium sp. MH3]
MNQTMERAQIEDILRSSKERGASDVHLTAGSPPVFRINGTLTPYGSEKMKPVIIDGFVRSLITEDMYQQLKQDRDIDFSF
GVTGVARYRVNCFYQRGALSMAFRTIPTEVPSLEQLGLPPVIKRFLTKPHGLILVTGPTGSGKSTTLAAMINEINQTMYK
RIITLEDPIEYLHSHRKSLIDQREIGIDAPRFSTGLRSALRQDPDVILLGEMRDLETIATAVTAAETGHLVFATVHTATA
ASTVSRIIDVFPSEQQDQIRAQLANVLAGVISQRLMPRSDGTGRVAALEIMVDTPAVSNLIRTGKEYQIQSVLQTGKQYG
MQTMQMALQDLVSRGLIPASAATPYISG

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=63500 U719_RS11590 WP_023468959.1 2180739..2181785(-) (pilT) [Exiguobacterium sp. MH3]
ATGAATCAAACGATGGAACGTGCCCAAATCGAAGATATTCTTCGTTCCTCGAAGGAACGAGGGGCATCCGATGTTCATTT
GACTGCCGGATCCCCTCCTGTGTTTCGGATCAATGGAACACTGACACCATATGGATCCGAGAAGATGAAACCAGTCATCA
TTGACGGGTTCGTTCGTTCGTTGATCACGGAAGACATGTATCAGCAACTGAAGCAGGATCGAGATATTGATTTTTCGTTT
GGTGTGACAGGTGTAGCACGATATCGGGTCAATTGTTTTTATCAGCGAGGCGCATTATCGATGGCCTTTCGAACGATCCC
GACAGAAGTGCCTTCGCTTGAACAGTTAGGACTTCCTCCAGTCATCAAGCGATTTTTGACGAAGCCACATGGTCTCATCC
TTGTCACGGGTCCAACAGGTTCCGGGAAGTCGACGACGCTTGCTGCGATGATTAACGAAATCAATCAAACAATGTATAAA
CGGATCATCACACTGGAAGACCCGATTGAATACTTACATAGTCATCGAAAGAGCTTAATTGATCAACGGGAGATTGGAAT
TGATGCGCCACGTTTTTCGACAGGATTGCGCTCAGCGCTTCGCCAAGATCCGGATGTTATTTTACTGGGAGAAATGCGCG
ACCTTGAGACGATTGCTACCGCGGTAACGGCTGCTGAAACCGGTCATCTTGTCTTTGCGACGGTGCATACGGCAACAGCA
GCCTCAACGGTCAGTCGCATCATCGATGTATTTCCGTCGGAGCAACAAGATCAGATTCGGGCACAGTTAGCGAACGTCCT
AGCGGGTGTCATCTCACAACGACTCATGCCACGATCGGATGGGACAGGACGTGTTGCGGCTTTAGAAATCATGGTTGATA
CACCAGCTGTCTCCAACTTGATTCGGACGGGTAAGGAATATCAGATTCAATCCGTTTTACAGACGGGTAAACAATATGGC
ATGCAAACGATGCAGATGGCTTTACAAGATTTGGTCAGCCGCGGTTTGATTCCGGCATCTGCTGCAACACCATACATATC
GGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0V8GJH8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.524

94.253

0.486
  pilT Neisseria meningitidis 8013

50.299

95.977

0.483
  pilT Neisseria gonorrhoeae MS11

50.299

95.977

0.483
  pilT Acinetobacter nosocomialis M2

49.552

96.264

0.477
  pilT Pseudomonas aeruginosa PAK

49.55

95.69

0.474
  pilT Acinetobacter baumannii D1279779

49.254

96.264

0.474
  pilT Acinetobacter baumannii strain A118

49.254

96.264

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.788

94.828

0.463
  pilT Vibrio cholerae strain A1552

48.788

94.828

0.463
  pilT Pseudomonas stutzeri DSM 10701

48.348

95.69

0.463
  pilT Acinetobacter baylyi ADP1

48.78

94.253

0.46
  pilT Legionella pneumophila strain Lp02

46.547

95.69

0.445
  pilT Legionella pneumophila strain ERS1305867

46.547

95.69

0.445
  pilU Acinetobacter baylyi ADP1

40.299

96.264

0.388
  pilU Pseudomonas stutzeri DSM 10701

39.514

94.54

0.374

Multiple sequence alignment