Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LQF09_RS03370 Genome accession   NZ_CP088935
Coordinates   751177..752310 (-) Length   377 a.a.
NCBI ID   WP_002930380.1    Uniprot ID   A0A351A1K6
Organism   Thauera sp. WB-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 746177..757310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQF09_RS03360 (LQF09_03360) - 748137..749789 (-) 1653 WP_270069211.1 FecR domain-containing protein -
  LQF09_RS03365 (LQF09_03365) - 749978..751144 (+) 1167 WP_270069212.1 class I SAM-dependent RNA methyltransferase -
  LQF09_RS03370 (LQF09_03370) pilU 751177..752310 (-) 1134 WP_002930380.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LQF09_RS03375 (LQF09_03375) pilT 752433..753473 (-) 1041 WP_002930383.1 type IV pilus twitching motility protein PilT Machinery gene
  LQF09_RS03380 (LQF09_03380) - 753566..754273 (+) 708 WP_270069213.1 YggS family pyridoxal phosphate-dependent enzyme -
  LQF09_RS03385 (LQF09_03385) proC 754332..755156 (+) 825 WP_270069214.1 pyrroline-5-carboxylate reductase -
  LQF09_RS03390 (LQF09_03390) - 755159..755740 (+) 582 WP_002940003.1 YggT family protein -
  LQF09_RS03395 (LQF09_03395) - 755793..757073 (-) 1281 WP_270069215.1 dihydroorotase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42437.81 Da        Isoelectric Point: 6.4201

>NTDB_id=634068 LQF09_RS03370 WP_002930380.1 751177..752310(-) (pilU) [Thauera sp. WB-2]
MERDQALRFMHDLLRLMLQKNGSDLFITTGFPPAIKIDGRIMPQSNQMLQPAHTAELARAIMNDRQAAEFESHKECNFGI
SPAGIGRFRVNAFVQQGKVGLVLRTIPQRIPKFEELGLPPVLSDIALTKRGLVIFVGGTGTGKTTSLASMVDYRNENTYG
HIITVEDPIEFVHAHKNCIVTQREVGLDTESWESALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRQQLLMDLSLNLRAMVSQRLLPLKDRKGRVPAVEVLLNSPLISDLIFKGDIPAIKEIMKRSTELG
MQTFDQSLFQLYEDELISYEDALRNADSVNDLRLQIKLNSRHGERDTSDSLKNLGML

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=634068 LQF09_RS03370 WP_002930380.1 751177..752310(-) (pilU) [Thauera sp. WB-2]
ATGGAACGCGACCAGGCACTCAGGTTCATGCACGATCTGCTGCGGCTGATGCTGCAGAAGAACGGCTCCGACCTGTTCAT
CACCACCGGCTTCCCGCCAGCGATCAAGATCGACGGCCGCATCATGCCGCAATCGAACCAGATGCTGCAGCCCGCACATA
CCGCCGAGCTTGCGCGCGCGATCATGAACGACCGCCAGGCCGCCGAGTTCGAGTCGCACAAGGAGTGCAACTTCGGCATC
TCGCCAGCCGGCATCGGGCGCTTCCGGGTCAACGCCTTCGTGCAGCAAGGCAAGGTCGGCCTGGTGCTGCGCACCATCCC
GCAGCGCATCCCCAAGTTCGAAGAGCTGGGCCTGCCCCCGGTGCTCAGCGATATCGCGCTGACCAAGCGCGGCCTGGTGA
TCTTCGTCGGTGGCACCGGCACCGGCAAGACCACCTCGCTCGCCTCGATGGTGGACTACCGCAACGAGAACACCTACGGC
CACATCATCACCGTCGAGGACCCGATCGAGTTCGTGCATGCACACAAGAACTGCATCGTCACCCAACGCGAGGTCGGCCT
CGACACCGAGTCCTGGGAGAGCGCACTCAAGAACACCCTGCGCCAGGCACCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGACTACGCCATCGCGTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCAAACAGCGCA
AACCAGGCCATCGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCCAGCAGCTGCTGATGGACCTGTCTCTGAACCT
GCGCGCAATGGTGTCGCAGCGCCTGCTGCCCCTGAAGGACCGCAAGGGCCGGGTGCCGGCGGTCGAGGTACTGCTCAACT
CGCCGCTGATCTCCGACCTCATCTTCAAGGGCGACATCCCGGCAATCAAGGAGATCATGAAGCGCTCGACCGAGCTCGGC
ATGCAGACCTTCGACCAGTCGCTGTTCCAGCTCTATGAGGACGAGCTCATCAGCTACGAGGACGCGCTGCGCAACGCCGA
CTCCGTCAATGACCTGCGCCTGCAGATCAAGCTCAACAGCCGCCACGGCGAACGCGACACCAGCGACAGCCTGAAGAACC
TAGGCATGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A351A1K6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.788

95.225

0.607
  pilU Acinetobacter baylyi ADP1

59.312

92.573

0.549
  pilU Vibrio cholerae strain A1552

56.145

94.96

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.672

88.859

0.406
  pilT Legionella pneumophila strain Lp02

43.807

87.798

0.385
  pilT Legionella pneumophila strain ERS1305867

43.807

87.798

0.385
  pilT Acinetobacter baylyi ADP1

42.433

89.39

0.379
  pilT Pseudomonas stutzeri DSM 10701

41.791

88.859

0.371
  pilT Pseudomonas aeruginosa PAK

41.791

88.859

0.371
  pilT Acinetobacter nosocomialis M2

42.296

87.798

0.371
  pilT Acinetobacter baumannii D1279779

42.296

87.798

0.371
  pilT Acinetobacter baumannii strain A118

42.296

87.798

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.812

84.881

0.363
  pilT Vibrio cholerae strain A1552

42.812

84.881

0.363