Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LRS40_RS10950 Genome accession   NZ_CP088265
Coordinates   1979246..1980223 (+) Length   325 a.a.
NCBI ID   WP_156264604.1    Uniprot ID   -
Organism   Leclercia sp. G3L     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1974246..1985223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRS40_RS10920 (LRS40_10920) - 1974642..1975586 (-) 945 WP_370649962.1 TRAP transporter substrate-binding protein -
  LRS40_RS10925 (LRS40_10925) hemW 1975920..1977056 (-) 1137 WP_142487050.1 radical SAM family heme chaperone HemW -
  LRS40_RS10930 (LRS40_10930) - 1977049..1977642 (-) 594 WP_142487049.1 XTP/dITP diphosphatase -
  LRS40_RS10935 (LRS40_10935) yggU 1977646..1977942 (-) 297 WP_103822821.1 DUF167 family protein YggU -
  LRS40_RS10940 (LRS40_10940) - 1977939..1978505 (-) 567 WP_231313692.1 YggT family protein -
  LRS40_RS10945 (LRS40_10945) - 1978527..1979228 (-) 702 WP_156264605.1 YggS family pyridoxal phosphate-dependent enzyme -
  LRS40_RS10950 (LRS40_10950) pilT 1979246..1980223 (+) 978 WP_156264604.1 type IV pilus twitching motility protein PilT Machinery gene
  LRS40_RS10955 (LRS40_10955) - 1980310..1980999 (+) 690 WP_231313693.1 IclR family transcriptional regulator -
  LRS40_RS10960 (LRS40_10960) ruvX 1981038..1981454 (-) 417 WP_142487044.1 Holliday junction resolvase RuvX -
  LRS40_RS10965 (LRS40_10965) - 1981454..1982014 (-) 561 WP_142487043.1 YqgE/AlgH family protein -
  LRS40_RS10970 (LRS40_10970) gshB 1982106..1983053 (-) 948 WP_231313694.1 glutathione synthase -
  LRS40_RS10975 (LRS40_10975) rsmE 1983073..1983804 (-) 732 WP_142490023.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LRS40_RS10980 (LRS40_10980) endA 1983916..1984623 (-) 708 WP_156264599.1 deoxyribonuclease I -
  LRS40_RS10985 (LRS40_10985) - 1984718..1985215 (-) 498 WP_156264598.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35847.05 Da        Isoelectric Point: 6.6296

>NTDB_id=633578 LRS40_RS10950 WP_156264604.1 1979246..1980223(+) (pilT) [Leclercia sp. G3L]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGEMEFAPFPGPDVVMLLKRWLSDEQQGAWCAQGQVDFAVTLEHHRLRAS
AFAHTQGCSLALRLLPEKCPQLCTLDTPRAIPELLTSDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCPSFADGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDAF
PAEEKEPVRKQLAGSLRAVLAQKLERDNQQGRVALFELLVNTPAAANLIREGKTWQLPGVIQTGLQAGMQNFEQSRAERR
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=633578 LRS40_RS10950 WP_156264604.1 1979246..1980223(+) (pilT) [Leclercia sp. G3L]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCACCACCGCG
CTGGCGGCGGCGAGGGGAAATGGAATTCGCCCCTTTTCCGGGGCCGGATGTGGTGATGCTGCTCAAACGCTGGCTCAGCG
ACGAACAGCAGGGGGCATGGTGCGCCCAGGGGCAGGTGGACTTTGCCGTTACACTGGAGCACCACCGGCTGCGGGCCAGC
GCCTTCGCCCATACGCAAGGGTGTTCGCTGGCGCTCCGGCTGTTGCCCGAAAAGTGCCCTCAGCTGTGTACCCTGGACAC
GCCGCGAGCGATCCCCGAGTTGCTCACCAGCGATAACGGGCTGATTCTGGTAACCGGGGCCACCGGCAGCGGTAAATCCA
CCACGCTTGCGGCCATGGTTGACTTTCTCAATCACCACAGCGACGGGCATATCCTGACCCTGGAAGATCCGGTGGAGTTC
ATCCATCAGAGCAAGCGTTGTCTGGTGCAGCAGCGGGAGATTGGCCTGCATTGCCCCTCTTTTGCCGATGGGCTTCGGGC
TGCGCTGCGTGAAGATCCTGACGTGATCCTGTTAGGTGAACTGCGCGACAGCGAAACGATCCGCCTTGCGCTCACCGCCG
CAGAGACCGGACATCTGGTGCTGGCGACCCTGCATACCCGGGGGGCCGCCCAGGCGATAGAGCGGCTGGTGGATGCCTTC
CCGGCAGAGGAGAAAGAGCCGGTACGTAAGCAGCTGGCTGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTCGAAAGGGA
TAACCAGCAGGGACGCGTCGCGCTGTTCGAGCTCCTGGTTAACACCCCTGCGGCGGCCAATTTGATCCGCGAAGGTAAAA
CCTGGCAACTGCCCGGCGTGATCCAGACCGGGTTGCAGGCAGGGATGCAGAACTTTGAGCAGAGCCGTGCTGAACGCAGG
GCACAGGGTCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.498
  pilT Vibrio cholerae strain A1552

49.541

100

0.498
  pilT Neisseria meningitidis 8013

47.188

98.462

0.465
  pilT Pseudomonas stutzeri DSM 10701

47.634

97.538

0.465
  pilT Legionella pneumophila strain Lp02

46.894

99.077

0.465
  pilT Legionella pneumophila strain ERS1305867

46.894

99.077

0.465
  pilT Acinetobacter baylyi ADP1

45.872

100

0.462
  pilT Acinetobacter nosocomialis M2

47.022

98.154

0.462
  pilT Acinetobacter baumannii strain A118

47.022

98.154

0.462
  pilT Acinetobacter baumannii D1279779

47.022

98.154

0.462
  pilT Neisseria gonorrhoeae MS11

46.875

98.462

0.462
  pilT Pseudomonas aeruginosa PAK

46.688

97.538

0.455
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.312

96.615

0.418
  pilU Vibrio cholerae strain A1552

40.252

97.846

0.394
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.378